LOCUS       sequence-339838-        5669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ExbB delta(235-244),ExbD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5669)
  TITLE     Postle Lab Plasmids
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKP1912
FEATURES             Location/Qualifiers
     source          1..5669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1208..1225)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1378..1464
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1556..1583
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1602..1693
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1694..1762,1763..2554)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1912..1931)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2596..3051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2683..2702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2893..2914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      3162..3750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3651..3670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3904..3921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4087..4109
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4247..4266
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(4446..5324)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5351..5635
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     5558..5577
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 atgggtaata atttaatgca gacggacctt tccgtctggg gtatgtatca gcacgccgat
       61 attgtcgtta agtgcgtgat gattgggctt attttggcct ccgtagtcac ctgggcaatc
      121 ttcttcagta agagcgtaga gttcttcaat cagaagcgtc gccttaagcg cgagcagcaa
      181 ctgctggctg aagcgcgttc cttaaaccag gccaacgata tcgccgctga ttttggtagc
      241 aaaagcttaa gcctgcattt gctcaatgaa gcgcagaatg agctggaact gtcagaaggc
      301 agcgacgata acgaaggtat taaagaacgt accagcttcc gtctggagcg tcgggtcgcc
      361 gcagtgggtc gtcaaatggg ccgcggtaac ggctatctgg caaccatcgg cgcgatttct
      421 ccgttcgttg gtctgtttgg tacggtctgg ggcatcatga acagctttat tggtatcgcg
      481 caaacgcaga ccactaacct ggcagtcgtt gcgccgggta tcgcagaagc tctgttagca
      541 acggcaatcg gcctcgtggc agcgattcct gcggtcgtta tctataacgt atttgcacgc
      601 cagattggcg gctttaaagc gatgctgggt gatgttgcag cgcaggtatt gttgctgcaa
      661 agccgtgacc tggatctgga agccagcgcc gctgcgcatc cgtaatatcc gatggcaatg
      721 catcttaacg aaaacctcga cgataacggc gaaatgcatg atatcaacgt gacgccgttt
      781 atcgacgtga tgttggttct gctgattatc tttatggtgg cggcaccgtt agcgacggta
      841 gatgtgaagg tgaacttgcc tgcttctacc agcacgccgc agccgcgccc ggaaaaaccg
      901 gtttatctgt cggtgaaggc agacaactcg atgtttatcg gtaacgatcc ggtcaccgat
      961 gaaacaatga ttacggcgtt gaatgcgtta accgaaggca agaaagacac caccatcttc
     1021 ttccgagcgg acaaaaccgt cgattacgag acgttgatga aggtaatgga tacgctgcat
     1081 caggcgggtt acctgaagat aggtctggtc ggcgaagaaa ccgccaaagc gaagtaaggg
     1141 gatcctctag agtcgacctg caggcatgca agcttggctg ttttggcgga tgagagaaga
     1201 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
     1261 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
     1321 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
     1381 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1441 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
     1501 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
     1561 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat
     1621 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1681 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1741 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1801 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1861 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1921 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     1981 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     2041 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     2101 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     2161 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     2221 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     2281 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2341 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2401 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2461 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2521 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2581 actttagatt gatttacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2641 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2701 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2761 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     2821 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     2881 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     2941 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3001 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt taaaaggatc
     3061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3301 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3781 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3841 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3901 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     3961 catctgtgcg gtatttcaca ccgcataggg tcatggctgc gccccgacac ccgccaacac
     4021 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     4081 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     4141 agaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     4201 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     4261 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     4321 agaggatctg ctcatgtttg acagcttatc atcgatgcat aatgtgcctg tcaaatggac
     4381 gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt
     4441 accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
     4501 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
     4561 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
     4621 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
     4681 aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
     4741 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
     4801 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
     4861 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
     4921 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
     4981 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
     5041 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
     5101 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
     5161 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     5221 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
     5281 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
     5341 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
     5401 tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa
     5461 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
     5521 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
     5581 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg
     5641 tttttttggg ctagcaggag gaattcacc
//
