LOCUS       sequence-339840-        5699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ExbB (235-244 Ala),ExbD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5699)
  TITLE     Postle Lab Plasmids
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKP2010
FEATURES             Location/Qualifiers
     source          1..5699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1238..1255)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1408..1494
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1586..1613
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1632..1723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1724..1792,1793..2584)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1942..1961)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2626..3081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2713..2732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2923..2944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      3192..3780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3681..3700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3934..3951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4117..4139
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4277..4296
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(4476..5354)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5381..5665
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     5588..5607
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 atgggtaata atttaatgca gacggacctt tccgtctggg gtatgtatca gcacgccgat
       61 attgtcgtta agtgcgtgat gattgggctt attttggcct ccgtagtcac ctgggcaatc
      121 ttcttcagta agagcgtaga gttcttcaat cagaagcgtc gccttaagcg cgagcagcaa
      181 ctgctggctg aagcgcgttc cttaaaccag gccaacgata tcgccgctga ttttggtagc
      241 aaaagcttaa gcctgcattt gctcaatgaa gcgcagaatg agctggaact gtcagaaggc
      301 agcgacgata acgaaggtat taaagaacgt accagcttcc gtctggagcg tcgggtcgcc
      361 gcagtgggtc gtcaaatggg ccgcggtaac ggctatctgg caaccatcgg cgcgatttct
      421 ccgttcgttg gtctgtttgg tacggtctgg ggcatcatga acagctttat tggtatcgcg
      481 caaacgcaga ccactaacct ggcagtcgtt gcgccgggta tcgcagaagc tctgttagca
      541 acggcaatcg gcctcgtggc agcgattcct gcggtcgtta tctataacgt atttgcacgc
      601 cagattggcg gctttaaagc gatgctgggt gatgttgcag cgcaggtatt gttgctgcaa
      661 agccgtgacc tggatctgga agccagcgcc gctgcgcatc cggcagctgc tgcagctgct
      721 gcagctgcag cttaatatcc gatggcaatg catcttaacg aaaacctcga cgataacggc
      781 gaaatgcatg atatcaacgt gacgccgttt atcgacgtga tgttggttct gctgattatc
      841 tttatggtgg cggcaccgtt agcgacggta gatgtgaagg tgaacttgcc tgcttctacc
      901 agcacgccgc agccgcgccc ggaaaaaccg gtttatctgt cggtgaaggc agacaactcg
      961 atgtttatcg gtaacgatcc ggtcaccgat gaaacaatga ttacggcgtt gaatgcgtta
     1021 accgaaggca agaaagacac caccatcttc ttccgagcgg acaaaaccgt cgattacgag
     1081 acgttgatga aggtaatgga tacgctgcat caggcgggtt acctgaagat aggtctggtc
     1141 ggcgaagaaa ccgccaaagc gaagtaaggg gatcctctag agtcgacctg caggcatgca
     1201 agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac
     1261 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
     1321 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
     1381 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1441 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     1501 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     1561 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
     1621 ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1681 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     1741 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1801 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1861 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1921 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     1981 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2041 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     2101 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     2161 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     2221 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     2281 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     2341 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     2401 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     2461 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     2521 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     2581 gtaactgtca gaccaagttt actcatatat actttagatt gatttacgcg ccctgtagcg
     2641 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2701 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2761 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2821 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     2881 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2941 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     3001 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3061 aaatattaac gtttacaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3121 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3301 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3541 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3661 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3721 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3781 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3841 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3901 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3961 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataggg
     4021 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4081 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4141 tttcaccgtc atcaccgaaa cgcgcgaggc agaaggagat ggcgcccaac agtcccccgg
     4201 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     4261 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     4321 cggtgatgcc ggccacgatg cgtccggcgt agaggatctg ctcatgtttg acagcttatc
     4381 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
     4441 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
     4501 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
     4561 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
     4621 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
     4681 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
     4741 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
     4801 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
     4861 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
     4921 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
     4981 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
     5041 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
     5101 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
     5161 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
     5221 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
     5281 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
     5341 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     5401 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     5461 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     5521 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     5581 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     5641 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacc
//
