LOCUS       sequence-33985-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Hogg JR, Collins K
  TITLE     RNA-based affinity purification reveals 7SK RNPs with distinct 
            composition and regulation.
  JOURNAL   RNA. 2007 Jun . 13(6):868-80.
  PUBMED    17456562
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2007 
            Jun . 13(6):868-80."
COMMENT     Sequence Label: pETZZtevPP7CP(C68A,C73A)
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..27)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(436..456)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(514..687)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
ORIGIN
        1 cggatctcag tggtggtggt ggtggtgctc gagggaacgg cccagcggca caaggttgac
       61 gacaagatct tcgacctgcg aggtcgcgac gagggacttg gtcaaatcgt acaacgattt
      121 gcgcgaggcc tcggtgctat tcgcaacgat tgtcacgtcg tgcgaccata cctgagtgta
      181 gcgcactttc ggaagctcgc cggcgacgct ggtggatgca tcaacgacgt ccgcctgatc
      241 cagttttagg ttgacgcgat acgcggtctt ggctccgttt tgacggagcg aagccgtgag
      301 gcgcagccga cccaccagag gcccgacctt ctcttcgaag atctgacggt ctgcggtgga
      361 ctggatctca gtcagagtgc gagtagcctc gccgaccgca agaacgatgg ttttggccat
      421 gctagccata tgggaaccct gaaaatacaa attctcgcta gcagtagttg gaatatcata
      481 atcggttgac ttccccgcgg aattcgcgtc tactttcggc gcctgagcac catttagctt
      541 tttagcttct gctaaaaggt tagcgctttg gcttgggtca tcttttaaac tttggatgaa
      601 ggcgtttcgt tgttcttcgt ttaagttagg taaatgtaag atctcataga acgcgttttg
      661 ttgttctttg ttgaatttgt tgtctacttt cggcgcctga gcatcattta gctttttagc
      721 ttctgctaaa aggttagcgc tttggcttgg gtcatctttt aaactttgga tgaaggcgtt
      781 tcgttgttct tcgtttaagt taggtaaatg taagatctca tagaacgcgt tttgttgttc
      841 tttgtgatt
//
