LOCUS       sequence-339853-        1788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for glutamate, third generation, variant 857 in
            GPI backbone. iGluSnFR3.v857.GPI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Aggarwal A, Liu R, Chen Y, Ralowicz AJ, Bergerson SJ, Tomaska F, 
            Mohar B, Hanson TL, Hasseman JP, Reep D, Tsegaye G, Yao P, Ji X, 
            Kloos M, Walpita D, Patel R, Mohr MA, Tillberg PW, Looger LL, Marvin
            JS, Hoppa MB, Konnerth A, Kleinfeld D, Schreiter ER, Podgorski K
  TITLE     Glutamate indicators with improved activation kinetics and 
            localization for imaging synaptic transmission.
  JOURNAL   Nat Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6.
  PUBMED    37142767
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 May 4. doi: 10.1038/s41592-023-01863-6."
COMMENT     Sequence Label: pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt
FEATURES             Location/Qualifiers
     source          1..1788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1639..1668
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacggaagcg ctgcaggatc aacactggac aaaattgcta aaaatggtgt catagtagtt
      121 ggacacagag actcttccgt accattttct tattacgata gtcagcataa ggtggtcgga
      181 tattcacaag aatacagcaa tgccatcgtg gaagctgtga agaagaaact gaacaaacca
      241 gatcttcaag ttaagttgat tccaattact tcccagaatc ggatacccct cctgcaaaac
      301 gggacttatg atttcgagtg cggtagtact acaaacaacg tcgaacgaca aaagcaagca
      361 gctttttctg acactatctt tgttgtggga accagactgc tcaccaagaa gggaggggat
      421 attaaagact ttgccaatct gaaggataag gcagtggtgg tgactagtgg aacaacttca
      481 gaggttttgc tgaacaaact caatgaggaa caaaaaatga acatgcgcat tattagcgca
      541 aaagatcatg gcgattcctt cagaacattg gaaagtgggc gcgctgttgc cttcatgatg
      601 gatgacagcc tgctcgccgg agaacgcgcc aaggcaaaga aaccagatga ttgggagatc
      661 gtcggcaagc cacaaagcca ggaggcttat gggtgcatgc tcaggaaaga tgacccccaa
      721 tttaagaagc tgatggatga cactatagca caagtccaaa cctccggaga agctgagaag
      781 tggttcgaca aatggtttaa aaaccccatc ctggtcaacc ataccgtcta tatcacagcc
      841 gataagcaga aaaatggaat taaagccaat tttaagatcc accataatgt agaagatggg
      901 agcgtgcagt tggcagacca ctaccagcaa aatacaccta tcggggatgg tccagtgctg
      961 ctgcctgaca accactatct ctcatatcaa tctgtgttgt caaaagatcc aaatgaaaaa
     1021 agggaccata tggtgctgct cgaatttgta acagccacag ggaactcact gggcatggac
     1081 gagctctaca agggagggac tggcggctca atgtccaaag gggaagaatt gtttaccgga
     1141 gtcgtgccta tccttgtcga actggatgga gatgttaatg ggcacaaatt ctccgtacgc
     1201 ggcgagggcg aaggggacgc cactaatggg aagctgactc ttaaatttat ctgcacaact
     1261 ggtaaattgc cagtgccctg gcctaccctt gtcaccacct tgagctacgg cgtccagtgt
     1321 ttcgcaaggt atccagacca tatgaagcaa catgactttt ttaaatccgc tatgcccgag
     1381 ggttacgtac aagagagaac catcagtttc aaagatgatg gtacttacaa aactcgcgca
     1441 gaagtcaagc tcgaaggaga cacccttgta aatcgaatag agttgaaggg cattgatttt
     1501 aaggaagacg gtaatatcct cggccacaaa ctcgagtata attttaacaa ccccttgaat
     1561 atgaactttg aactctcaga tgaaatgaaa gccctgttta aagagccaaa cgacaaagca
     1621 cttaaactgc aggtcgacga acaaaaactc atctcagaag aggatctgtc agataacgac
     1681 tacggtaaag atgggttggc tggggcttca ggaggcagac gaaatggcgc tataactgtg
     1741 ctcagtttca taacgttcta cgtggcagca tttatggtat tgttgtag
//
