LOCUS       sequence-33986-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Hogg JR, Collins K
  TITLE     RNA-based affinity purification reveals 7SK RNPs with distinct 
            composition and regulation.
  JOURNAL   RNA. 2007 Jun . 13(6):868-80.
  PUBMED    17456562
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2007 
            Jun . 13(6):868-80."
COMMENT     Sequence Label: pETZZtevPP7CP(C68A,C73A)
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             75..248
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             249..422
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             480..500
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tagaataatt ttgtttaact ttaagaagga gatataccat ggaaaccgcg gctcttgcgc
       61 aacacgatga agccgtggac aacaaattca acaaagaaca acaaaacgcg ttctatgaga
      121 tcttacattt acctaactta aacgaagaac aacgaaacgc cttcatccaa agtttaaaag
      181 atgacccaag ccaaagcgct aaccttttag cagaagctaa aaagctaaat gatgctcagg
      241 cgccgaaagt agacaacaaa ttcaacaaag aacaacaaaa cgcgttctat gagatcttac
      301 atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta aaagatgacc
      361 caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatggtgct caggcgccga
      421 aagtagacgc gaattccgcg gggaagtcaa ccgattatga tattccaact actgctagcg
      481 agaatttgta ttttcagggt tcccatatgg ctagcatggc caaaaccatc gttcttgcgg
      541 tcggcgaggc tactcgcact ctgactgaga tccagtccac cgcagaccgt cagatcttcg
      601 aagagaaggt cgggcctctg gtgggtcggc tgcgcctcac ggcttcgctc cgtcaaaacg
      661 gagccaagac cgcgtatcgc gtcaacctaa aactggatca ggcggacgtc gttgatgcat
      721 ccaccagcgt cgccggcgag cttccgaaag tgcgctacac tcangtatgg tcgcacgacg
      781 tgacaatcgt tgcgaatagc accgaggcct cgcgcaaatc gttgtacgat ttgaccaagt
      841 ccct
//
