LOCUS       sequence-339872-        7683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces T7 RNA Polymerase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Schmidt CM, Shis DL, Nguyen-Huu TD, Bennett MR
  TITLE     Stable maintenance of multiple plasmids in E. coli using a single 
            selective marker.
  JOURNAL   ACS Synth Biol. 2012 Oct 19;1(10):445-50. doi: 10.1021/sb3000589. 
            Epub 2012 Sep 4.
  PUBMED    23656183
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb3000589";
            journalName: "ACS Synth Biol"; date: "2012-10-19- 19"; volume: "1"; 
            issue: "10"; pages: "445-50"
COMMENT     Sequence Label: pCS6
FEATURES             Location/Qualifiers
     source          1..7683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(191..1069)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1096..1380
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1303..1322
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(4140..4157)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(4903..5853)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5901..6123
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(6615..6709)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6753..7544)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
       61 gaccagttgc gtgagcgcat acgctacttg cattacagct tacgaaatgt gcctgtcaaa
      121 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
      181 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
      241 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
      301 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
      361 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
      421 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
      481 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
      541 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
      601 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
      661 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
      721 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
      781 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
      841 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
      901 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
      961 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
     1021 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
     1081 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
     1141 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     1201 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     1261 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     1321 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     1381 acccgttttt ttgggctagc gaattcgagc tcaagaggat accatatgaa cacgattaac
     1441 atcgctaaga acgacttctc tgacatcgaa ctggctgcta tcccgttcaa cactctggct
     1501 gaccattacg gtgagcgttt agctcgcgaa cagttggccc ttgagcatga gtcttacgag
     1561 atgggtgaag cacgcttccg caagatgttt gagcgtcaac ttaaagctgg tgaggttgcg
     1621 gataacgctg ccgccaagcc tctcatcact accctactcc ctaagatgat tgcacgcatc
     1681 aacgactggt ttgaggaagt gaaagctaag cgcggcaagc gcccgacagc cttccagttc
     1741 ctgcaagaaa tcaagccgga agccgtagcg tacatcacca ttaagaccac tctggcttgc
     1801 ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa gcgcaatcgg tcgggccatt
     1861 gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag ctaagcactt caagaaaaac
     1921 gttgaggaac aactcaacaa gcgcgtaggg cacgtctaca agaaagcatt tatgcaagtt
     1981 gtcgaggctg acatgctctc taagggtcta ctcggtggcg aggcgtggtc ttcgtggcat
     2041 aaggaagact ctattcatgt aggagtacgc tgcatcgaga tgctcattga gtcaaccgga
     2101 atggttagct tacaccgcca aaatgctggc gtagtaggtc aagactctga gactatcgaa
     2161 ctcgcacctg aatacgctga ggctatcgca acccgtgcag gtgcgctggc tggcatctct
     2221 ccgatgttcc aaccttgcgt agttcctcct aagccgtgga ctggcattac tggtggtggc
     2281 tattgggcta acggtcgtcg tcctctggcg ctggtgcgta ctcacagtaa gaaagcactg
     2341 atgcgctacg aagacgttta catgcctgag gtgtacaaag cgattaacat tgcgcaaaac
     2401 accgcatgga aaatcaacaa gaaagtccta gcggtcgcca acgtaatcac caagtggaag
     2461 cattgtccgg tcgaggacat ccctgcgatt gagcgtgaag aactcccgat gaaaccggaa
     2521 gacatcgaca tgaatcctga ggctctcacc gcgtggaaac gtgctgccgc tgctgtgtac
     2581 cgcaaggaca aggctcgcaa gtctcgccgt atcagccttg agttcatgct tgagcaagcc
     2641 aataagtttg ctaaccataa ggccatctgg ttcccttaca acatggactg gcgcggtcgt
     2701 gtttacgctg tgtcaatgtt caacccgcaa ggtaacgata tgaccaaagg actgcttacg
     2761 ctggcgaaag gtaaaccaat cggtaaggaa ggttactact ggctgaaaat ccacggtgca
     2821 aactgtgcgg gtgtcgataa ggttccgttc cctgagcgca tcaagttcat tgaggaaaac
     2881 cacgagaaca tcatggcttg cgctaagtct ccactggaga acacttggtg ggctgagcaa
     2941 gattctccgt tctgcttcct tgcgttctgc tttgagtacg ctggggtaca gcaccacggc
     3001 ctgagctata actgctccct tccgctggcg tttgacgggt cttgctctgg catccagcac
     3061 ttctccgcga tgctccgaga tgaggtaggt ggtcgcgcgg ttaacttgct tcctagtgaa
     3121 accgttcagg acatctacgg gattgttgct aagaaagtca acgagattct acaagcagac
     3181 gcaatcaatg ggaccgataa cgaagtagtt accgtgaccg atgagaacac tggtgaaatc
     3241 tctgagaaag tcaagctggg cactaaggca ctggctggtc aatggctggc ttacggtgtt
     3301 actcgcagtg tgactaagcg ttcagtcatg acgctggctt acgggtccaa agagttcggc
     3361 ttccgtcaac aagtgctgga agataccatt cagccagcta ttgattccgg caagggtctg
     3421 atgttcactc agccgaatca ggctgctgga tacatggcta agctgatttg ggaatctgtg
     3481 agcgtgacgg tggtagctgc ggttgaagca atgaactggc ttaagtctgc tgctaagctg
     3541 ctggctgctg aggtcaaaga taagaagact ggagagattc ttcgcaagcg ttgcgctgtg
     3601 cattgggtaa ctcctgatgg tttccctgtg tggcaggaat acaagaagcc tattcagacg
     3661 cgcttgaacc tgatgttcct cggtcagttc cgcttacagc ctaccattaa caccaacaaa
     3721 gatagcgaga ttgatgcaca caaacaggag tctggtatcg ctcctaactt tgtacacagc
     3781 caagacggta gccaccttcg taagactgta gtgtgggcac acgagaagta cggaatcgaa
     3841 tcttttgcac tgattcacga ctccttcggt accattccgg ctgacgctgc gaacctgttc
     3901 aaagcagtgc gcgaaactat ggttgacaca tatgagtctt gtgatgtact ggctgatttc
     3961 tacgaccagt tcgctgacca gttgcacgag tctcaattgg acaaaatgcc agcacttccg
     4021 gctaaaggta acttgaacct ccgtgacatc ttagagtcgg acttcgcgtt cgcgtaatct
     4081 agagtcgacc tgcaggcatg caagcttggc tgttttggcg gatgagagaa gattttcagc
     4141 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc
     4201 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
     4261 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
     4321 aaaggctcag tcgaaagact gaacgctctc ctgagtagga caaatccgcc gccctagacc
     4381 tagggtacgg gttttgctgc ccgcaaacgg gctgttctgg tgttgctagt ttgttatcag
     4441 aatcgcagat ccggcttcag ccggtttgcc ggctgaaagc gctatttctt ccagaattgc
     4501 catgattttt tccccacggg aggcgtcact ggctcccgtg ttgtcggcag ctttgattcg
     4561 ataagcagca tcgcctgttt caggctgtct atgtgtgact gttgagctgt aacaagttgt
     4621 ctcaggtgtt caatttcatg ttctagttgc tttgttttac tggtttcacc tgttctatta
     4681 ggtgttacat gctgttcatc tgttacattg tcgatctgtt catggtgaac agctttgaat
     4741 gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt ttcatctgtg
     4801 catatggaca gttttccctt tgatatgtaa cggtgaacag ttgttctact tttgtttgtt
     4861 agtcttgatg cttcactgat agatacaaga gccataagaa cctcagatcc ttccgtattt
     4921 agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt
     4981 gagatcatac ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat
     5041 ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttatgtaggt aggaatctga
     5101 tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt
     5161 tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc
     5221 tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt
     5281 caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa
     5341 cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc
     5401 ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc
     5461 cagattatat tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa
     5521 aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa
     5581 gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt
     5641 agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt attggttata
     5701 agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc
     5761 cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag
     5821 ttcatttgct ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta
     5881 atcactatac caattgagat gggctagtca atgataatta ctagtccttt tcctttgagt
     5941 tgtgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc
     6001 ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat
     6061 ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa
     6121 ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc
     6181 tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa
     6241 atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg
     6301 acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc
     6361 gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct
     6421 tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc
     6481 agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc
     6541 agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg
     6601 tccctagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     6661 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     6721 gatatccgtc ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg
     6781 cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
     6841 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg
     6901 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
     6961 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
     7021 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
     7081 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
     7141 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
     7201 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
     7261 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
     7321 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
     7381 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
     7441 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
     7501 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt taactttgtt
     7561 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     7621 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag
     7681 tca
//
