LOCUS       sequence-339879-       15207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lenti vector encoding dCas9-hHDAC3 
            (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human 
            HDAC3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15207)
  AUTHORS   Kwon DY, Zhao YT, Lamonica JM, Zhou Z
  TITLE     Locus-specific histone deacetylation using a synthetic 
            CRISPR-Cas9-based HDAC.
  JOURNAL   Nat Commun. 2017 May 12;8:15315. doi: 10.1038/ncomms15315.
  PUBMED    28497787
REFERENCE   2  (bases 1 to 15207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-12"; volume: "8:15315. doi"; pages: " 
            10.1038/ncomms15315"
COMMENT     Sequence Label: dCas9-hHDAC3
FEATURES             Location/Qualifiers
     source          1..15207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        110..489
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        491..689
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     644..664
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             707..887
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    934..1059
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1552..1785
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1970..2014
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2312..2429
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2559..3737
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2790..3728
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3685..3705
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3753..3800
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(3801..3824,3825..3827,3828..6383,6384..6386,
                     6387..7901)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7956..7976
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9294..9347
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9348..9746
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9771..10359
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9824..9844)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10242..10253)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10362..10378)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10363..10379)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10884..11064
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11090..11107)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11096..11320
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11366..11794
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11453..11472)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11663..11684
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11803..11823)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11808..12137
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11988..12123
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12050..12069
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12185..12232
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12251..12625
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12755..12876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12792..12811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12846..12865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12925..12941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12925..12941)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12938..12960)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12949..12965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12973..12979,12980..12997,12998..13003))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13018..13039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13156..13173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13327..13915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13407..13426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14086..14877,14878..14946))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14709..14728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14947..15051)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15126..15145)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
       61 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      121 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      181 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      241 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      301 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      361 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      421 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      481 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      541 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      601 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      661 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
      721 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      781 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      841 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
      901 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
      961 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1021 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1081 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1141 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1201 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1261 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1321 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1381 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1441 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1501 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     1561 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     1621 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1681 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1741 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1801 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     1861 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     1921 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     1981 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2041 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     2101 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     2161 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     2221 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
     2281 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     2341 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2401 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     2461 tttggttaat tagctagctg caaagatgga taaagtttta aacagagagg aatctttgca
     2521 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
     2581 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2641 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2701 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2761 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2821 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2881 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2941 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     3001 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     3061 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     3121 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     3181 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     3241 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg
     3301 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     3361 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     3421 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     3481 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     3541 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3601 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3661 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3721 catttcaggt gtcgtgacgt acggccacca tgaaaaggcc ggcggccacg aaaaaggccg
     3781 gccaggcaaa aaagaaaaag gacaagaagt acagcatcgg cctggccatc ggcaccaact
     3841 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
     3901 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
     3961 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
     4021 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
     4081 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
     4141 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
     4201 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
     4261 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     4321 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     4381 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     4441 gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     4501 atggcctgtt cggcaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     4561 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     4621 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     4681 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     4741 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     4801 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     4861 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
     4921 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca
     4981 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     5041 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     5101 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     5161 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     5221 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
     5281 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     5341 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     5401 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
     5461 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
     5521 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
     5581 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
     5641 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
     5701 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
     5761 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
     5821 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
     5881 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
     5941 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
     6001 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
     6061 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
     6121 agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
     6181 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
     6241 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
     6301 actacgatgt ggaccacatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca
     6361 aggtgctgac cagaagcgac aaggcccggg gcaagagcga caacgtgccc tccgaagagg
     6421 tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga
     6481 gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
     6541 gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
     6601 tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag
     6661 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
     6721 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
     6781 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
     6841 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
     6901 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     6961 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
     7021 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
     7081 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     7141 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
     7201 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     7261 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     7321 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     7381 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     7441 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     7501 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
     7561 aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg
     7621 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca
     7681 acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc
     7741 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
     7801 ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
     7861 tgtacgagac acggatcgac ctgtctcagc tgggaggcga cagcgctgga ggaggtggaa
     7921 gcggaggagg aggaagcgga ggaggaggta gcggacctaa gaaaaagagg aaggtggcgg
     7981 ccgctggatc catggccaag accgtggcct atttctacga ccccgacgtg ggcaacttcc
     8041 actacggagc tggacaccct atgaagcctc atcgcctggc attgacccat agcctggtcc
     8101 tgcattacgg tctctataag aagatgatcg tcttcaagcc ataccaggcc tcccagcatg
     8161 acatgtgccg cttccactcc gaggactaca ttgacttcct gcagagagtc agccccacca
     8221 atatgcaagg cttcaccaag agtcttaatg ccttcaacgt aggcgatgac tgcccagtgt
     8281 ttcccgggct ctttgagttc tgctcgcgtt acacaggcgc atctctgcaa ggagcaaccc
     8341 agctgaacaa caagatctgt gatattgcca ttaactgggc tggtggtctg caccatgcca
     8401 agaagtttga ggcctctggc ttctgctatg tcaacgacat tgtgattggc atcctggagc
     8461 tgctcaagta ccaccctcgg gtgctctaca ttgacattga catccaccat ggtgacgggg
     8521 ttcaagaagc tttctacctc actgaccggg tcatgacggt gtccttccac aaatacggaa
     8581 attacttctt ccctggcaca ggtgacatgt atgaagtcgg ggcagagagt ggccgctact
     8641 actgtctgaa cgtgcccctg cgggatggca ttgatgacca gagttacaag caccttttcc
     8701 agccggttat caaccaggta gtggacttct accaacccac gtgcattgtg ctccagtgtg
     8761 gagctgactc tctgggctgt gatcgattgg gctgctttaa cctcagcatc cgagggcatg
     8821 gggaatgcgt tgaatatgtc aagagcttca atatccctct actcgtgctg ggtggtggtg
     8881 gttatactgt ccgaaatgtt gcccgctgct ggacatatga gacatcgctg ctggtagaag
     8941 aggccattag tgaggagctt ccctatagtg aatacttcga gtactttgcc ccagacttca
     9001 cacttcatcc agatgtcagc acccgcatcg agaatcagaa ctcacgccag tatctggacc
     9061 agatccgcca gacaatcttt gaaaacctga agatgctgaa ccatgcacct agtgtccaga
     9121 ttcatgacgt gcctgcagac ctcctgacct atgacaggac tgatgaggct gatgcagagg
     9181 agaggggtcc tgaggagaac tatagcaggc cagaggcacc caatgagttc tatgatggag
     9241 accatgacaa tgacaaggaa agcgatgtgg agatttaatg tacaggcagt ggagagggca
     9301 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg gccaagcctt
     9361 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
     9421 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
     9481 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
     9541 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
     9601 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
     9661 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
     9721 cctctggtta tgtgtgggag ggctaagaat tcgatatcaa gcttatcggt aatcaacctc
     9781 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     9841 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     9901 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     9961 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
    10021 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
    10081 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
    10141 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
    10201 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
    10261 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
    10321 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
    10381 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
    10441 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
    10501 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
    10561 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
    10621 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
    10681 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
    10741 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
    10801 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
    10861 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10921 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10981 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    11041 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
    11101 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
    11161 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
    11221 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
    11281 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
    11341 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
    11401 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    11461 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    11521 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    11581 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    11641 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    11701 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    11761 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
    11821 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    11881 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    11941 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    12001 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    12061 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    12121 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
    12181 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
    12241 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
    12301 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
    12361 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
    12421 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
    12481 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
    12541 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
    12601 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
    12661 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    12721 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    12781 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    12841 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    12901 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    12961 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    13021 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    13081 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    13141 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    13201 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    13261 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    13321 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    13381 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    13441 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    13501 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    13561 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    13621 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    13681 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    13741 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    13801 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    13861 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    13921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    13981 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    14041 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    14101 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    14161 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    14221 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    14281 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    14341 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    14401 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    14461 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    14521 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    14581 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    14641 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    14701 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    14761 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    14821 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    14881 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    14941 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    15001 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    15061 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
    15121 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
    15181 ttggaggtcg ctgagtagtg cgcgagc
//
