LOCUS       sequence-339890-       15020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into 
            plants by agrobacterium mediated transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15020)
  AUTHORS   Xie K, Yang Y
  TITLE     RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
  JOURNAL   Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 
            Aug 17.
  PUBMED    23956122
REFERENCE   2  (bases 1 to 15020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/mp/sst119";
            journalName: "Mol Plant"; date: "2013-11"; volume: "6"; issue: "6"; 
            pages: "1975-83"
COMMENT     Sequence Label: pRGEB31
FEATURES             Location/Qualifiers
     source          1..15020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             699..1724
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1765..1939
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2017..2041
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2466..3260
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3347..3935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3836..3855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4089..4106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4121..4261
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4187..4206)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4347..4369
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4605..4799
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4865..5938)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6367..6996)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8297..8321
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8509..8531
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8523..8540
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8524..8540
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(8549..8801)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8823..8846)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    8857..8881
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(8914..8961)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(8962..13062)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(8962..13062)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(13087..13107)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(13114..13179)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(13224..13248)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(13270..13615)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_RNA        complement(14181..14256)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(14273..14653)
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     primer_bind     complement(14671..14687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14671..14687)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14684..14706)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14695..14711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14719..14725,14726..14743,14744..14749))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14764..14785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14976..15020,1..632)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
       61 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      121 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      181 gtcttcaaag caagtggatt gatgtgaaca tggtggagca cgacactctc gtctactcca
      241 agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
      301 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga
      361 cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg
      421 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
      481 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
      541 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
      601 gaagttcatt tcatttggag aggacacgct gaaatcacca gtctctctct acaaatctat
      661 ctctctcgag ctttcgcaga tccggggggc aatgagatat gaaaaagcct gaactcaccg
      721 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
      781 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
      841 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
      901 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggagtttagc gagagcctga
      961 cctattgcat ctcccgccgt tcacagggtg tcacgttgca agacctgcct gaaaccgaac
     1021 tgcccgctgt tctacaaccg gtcgcggagg ctatggatgc gatcgctgcg gccgatctta
     1081 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     1141 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     1201 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     1261 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     1321 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     1381 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     1441 acttcgagcg gaggcatccg gagcttgcag gatcgccacg actccgggcg tatatgctcc
     1501 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     1561 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     1621 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     1681 gtggaaaccg acgccccagc actcgtccga gggcaaagaa atagagtaga tgccgaccgg
     1741 gatctgtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa
     1801 gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta
     1861 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
     1921 cagtactaaa atccagatcc cccgaattaa ttcggcgtta attcagtaca ttaaaaacgt
     1981 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     2041 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     2101 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     2161 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     2221 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac
     2281 cgcggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt
     2341 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     2401 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat
     2461 aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct
     2521 gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg
     2581 aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac
     2641 gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact
     2701 ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct
     2761 cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca
     2821 tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc
     2881 gcttagccga attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact
     2941 gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa
     3001 agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga
     3061 aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt
     3121 atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg
     3181 agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt
     3241 tactggatga attgttttag tacctagaat gcatgaccaa aatcccttaa cgtgagtttt
     3301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3481 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3961 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4021 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4081 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     4141 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     4201 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     4261 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     4321 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     4381 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     4441 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     4501 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     4561 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     4621 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     4681 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     4741 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctattcacag
     4801 gaaagagtgc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     4861 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     4921 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     4981 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     5041 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     5101 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     5161 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     5221 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     5281 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     5341 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     5401 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     5461 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
     5521 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
     5581 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
     5641 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
     5701 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
     5761 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
     5821 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
     5881 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc
     5941 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcaggcaa cccagccgct
     6001 tacgcctggc caaccgcccg ttcctcaaca catggggcat tccacggcgt cggtgcctgg
     6061 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
     6121 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
     6181 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
     6241 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
     6301 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
     6361 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
     6421 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
     6481 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
     6541 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
     6601 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
     6661 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
     6721 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
     6781 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
     6841 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
     6901 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
     6961 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
     7021 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
     7081 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
     7141 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
     7201 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
     7261 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
     7321 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
     7381 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
     7441 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
     7501 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
     7561 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
     7621 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
     7681 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
     7741 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
     7801 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
     7861 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
     7921 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
     7981 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
     8041 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
     8101 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
     8161 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
     8221 caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa
     8281 cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact
     8341 gaaggcggga aacgacaatc tgatccaagc tcaagctgct ctagcattcg ccattcaggc
     8401 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     8461 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     8521 gttgtaaaac gacggccagt gaattcccga tctagtaaca tagatgacac cgcgcgcgat
     8581 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     8641 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     8701 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     8761 ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggaaatt cgagctctaa
     8821 gccttgtcat cgtcatcctt gtagtcgctg ttatcaacca ctttgtacaa gaaagctggg
     8881 tctagatatc tcgagtgcgg ccgcgaattc ttactttttc ttttttgcct ggccggcctt
     8941 tttcgtggcc gccggccttt tgtcgcctcc cagctgagac aggtcgatcc gtgtctcgta
     9001 caggccggtg atgctctggt ggatcagggt ggcgtccagc acctctttgg tgctggtgta
     9061 cctcttccgg tcgatggtgg tgtcaaagta cttgaaggcg gcaggggctc ccagattggt
     9121 cagggtaaac aggtggatga tattctcggc ctgctctctg atgggcttat cccggtgctt
     9181 gttgtaggcg gacagcactt tgtccagatt agcgtcggcc aggatcactc tcttggagaa
     9241 ctcgctgatc tgctcgatga tctcgtccag gtagtgcttg tgctgttcca caaacagctg
     9301 tttctgctca ttatcctcgg gggagccctt cagcttctca tagtggctgg ccaggtacag
     9361 gaagttcaca tatttggagg gcagggccag ttcgtttccc ttctgcagtt cgccggcaga
     9421 ggccagcatt ctcttccggc cgttttccag ctcgaacagg gagtacttag gcagcttgat
     9481 gatcaggtcc tttttcactt ctttgtagcc cttggcttcc agaaagtcga tgggattctt
     9541 ctcgaagctg cttctttcca tgatggtgat ccccagcagc tctttcacac tcttcagttt
     9601 cttggacttg cccttttcca ctttggccac caccagcaca gaataggcca cggtggggct
     9661 gtcgaagccg ccgtacttct tagggtccca gtccttcttt ctggcgatca gcttatcgct
     9721 gttcctcttg ggcaggatag actctttgct gaagccgcct gtctgcacct cggtcttttt
     9781 cacgatattc acttggggca tgctcagcac tttccgcacg gtggcaaaat cccggccctt
     9841 atcccacacg atctccccgg tttcgccgtt tgtctcgatc agaggccgct tccggatctc
     9901 gccgttggcc agggtaatct cggtcttgaa aaagttcatg atgttgctgt agaagaagta
     9961 cttggcggta gccttgccga tttcctgctc gctcttggcg atcatcttcc gcacgtcgta
    10021 caccttgtag tcgccgtaca cgaactcgct ttccagctta gggtactttt tgatcagggc
    10081 ggttcccacg acggcgttca ggtaggcgtc gtgggcgtgg tggtagttgt tgatctcgcg
    10141 cactttgtaa aactggaaat ccttccggaa atcggacacc agcttggact tcagggtgat
    10201 cactttcact tcccggatca gcttgtcatt ctcgtcgtac ttagtgttca tccgggagtc
    10261 caggatctgt gccacgtgct ttgtgatctg ccgggtttcc accagctgtc tcttgatgaa
    10321 gccggcctta tccagttcgc tcaggccgcc tctctcggcc ttggtcagat tgtcgaactt
    10381 tctctgggta atcagcttgg cgttcagcag ctgccgccag tagttcttca tcttcttcac
    10441 gacctcttcg gagggcacgt tgtcgctctt gccccggttc ttgtcgcttc tggtcagcac
    10501 cttgttgtcg atggagtcgt ccttcagaaa gctctgaggc acgatatggt ccacatcgta
    10561 gtcggacagc cggttgatgt ccagttcctg gtccacgtac atatcccgcc cattctgcag
    10621 gtagtacagg tacagcttct cgttctgcag ctgggtgttt tccacggggt gttctttcag
    10681 gatctggctg cccagctctt tgatgccctc ttcgatccgc ttcattctct cgcggctgtt
    10741 cttctgtccc ttctgggtgg tctggttctc tctggccatt tcgatcacga tgttctcggg
    10801 cttgtgccgg cccatcactt tcacgagctc gtccaccacc ttcactgtct gcaggatgcc
    10861 cttcttaatg gcggggctgc cggccagatt ggcaatgtgc tcgtgcaggc tatcgccctg
    10921 gccggacacc tgggctttct ggatgtcctc tttaaaggtc aggctgtcgt cgtggatcag
    10981 ctgcatgaag tttctgttgg cgaagccgtc ggacttcagg aaatccagga ttgtcttgcc
    11041 ggactgcttg tcccggatgc cgttgatcag cttccggctc agcctgcccc agccggtgta
    11101 tctccgccgc ttcagctgct tcatcacttt gtcgtcgaac aggtgggcat aggttttcag
    11161 ccgttcctcg atcatctctc tgtcctcaaa cagtgtcagg gtcagcacga tatcttccag
    11221 aatgtcctcg ttttcctcat tgtccaggaa gtccttgtcc ttgataattt tcagcagatc
    11281 gtggtatgtg cccagggagg cgttgaaccg atcttccacg ccggagattt ccacggagtc
    11341 gaagcactcg attttcttga agtagtcctc tttcagctgc ttcacggtca ctttccggtt
    11401 ggtcttgaac agcaggtcca cgatggcctt tttctgctcg ccgctcagga aggcgggctt
    11461 tctcattccc tcggtcacgt atttcacttt ggtcagctcg ttatacacgg tgaagtactc
    11521 gtacagcagg ctgtgcttgg gcagcacctt ctcgttgggc aggttcttat cgaagttggt
    11581 catccgctcg atgaagctct gggcggaagc gcccttgtcc accacttcct cgaagttcca
    11641 gggggtgatg gtttcctcgc tctttctggt catccaggcg aatctgctgt ttcccctggc
    11701 cagagggccc acgtagtagg ggatgcggaa ggtcaggatc ttctcgatct tttcccggtt
    11761 gtccttcagg aatgggtaaa aatcttcctg ccgccgcaga atggcgtgca gctctcccag
    11821 gtggatctgg tgggggatgc tgccgttgtc gaaggtccgc tgcttccgca gcaggtcctc
    11881 tctgttcagc ttcacgagca gttcctcggt gccgtccatc ttttccagga tgggcttgat
    11941 gaacttgtag aactcttcct ggctggctcc gccgtcaatg tagccggcgt agccgttctt
    12001 gctctggtcg aagaaaatct ctttgtactt ctcaggcagc tgctgccgca cgagagcttt
    12061 cagcagggtc aggtcctggt ggtgctcgtc gtatctcttg atcatagagg cgctcagggg
    12121 ggccttggtg atctcggtgt tcactctcag gatgtcgctc agcaggatgg cgtcggacag
    12181 gttcttggcg gccagaaaca ggtcggcgta ctggtcgccg atctgggcca gcaggttgtc
    12241 caggtcgtcg tcgtaggtgt ccttgctcag ctgcagtttg gcatcctcgg ccaggtcgaa
    12301 gttgctcttg aagttggggg tcaggcccag gctcagggca atcaggtttc cgaacaggcc
    12361 attcttcttc tcgccgggca gctgggcgat cagattttcc agccgtctgc tcttgctcag
    12421 tctggcagac aggatggcct tggcgtccac gccgctggcg ttgatggggt tttcctcgaa
    12481 cagctggttg taggtctgca ccagctggat gaacagcttg tccacgtcgc tgttgtcggg
    12541 gttcaggtcg ccctcgatca ggaagtggcc ccggaacttg atcatgtggg ccagggccag
    12601 atagatcagc cgcaggtcgg ccttgtcggt gctgtccacc agtttctttc tcaggtggta
    12661 gatggtgggg tacttctcgt ggtaggccac ctcgtccacg atgttgccga agatggggtg
    12721 ccgctcgtgc ttcttatcct cttccaccag gaaggactct tccagtctgt ggaagaagct
    12781 gtcgtccacc ttggccatct cgttgctgaa gatctcttgc agatagcaga tccggttctt
    12841 ccgtctggtg tatcttcttc tggcggttct cttcagccgg gtggcctcgg ctgtttcgcc
    12901 gctgtcgaac agcagggctc cgatcaggtt cttcttgatg ctgtgccggt cggtgttgcc
    12961 cagcaccttg aatttcttgc tgggcacctt gtactcgtcg gtgatcacgg cccagcccac
    13021 agagttggtg ccgatgtcca ggccgatgct gtacttcttg tcggctgctg ggactccgtg
    13081 gataccgacc ttccgcttct tctttggggc catcttatcg tcatcgtctt tgtaatcaat
    13141 atcatgatcc ttgtagtctc cgtcgtggtc cttatagtcc atggttaagt atttccttag
    13201 agaattggtt ctatctcctt cgaagcctgc ttttttgtac aaacttgttg ataactctag
    13261 agtcccccgt gttctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga
    13321 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
    13381 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
    13441 atctttggga ccactgtcgg cagaggcatc ttcaacgatg gcctttcctt tatcgcaatg
    13501 atggcatttg taggagccac cttccttttc cactatcttc acaataaagt gacagatagc
    13561 tgggcaatgg aatccgagga ggtttccgga tattaccctt tgttgaaaag tctcaattgc
    13621 cctttggtct tctgagactg tatctttgat atttttggag tagacaagtg tgtcgtgctc
    13681 caccatgttg acgaagattt tcttcttgtc attgagtcgt aagagactct gtatgaactg
    13741 ttcgccagtc tttacggcga gttctgttag gtcctctatt tgaatctttg actccatggc
    13801 ctttgattca gtgggaacta cctttttaga gactccaatc tctattactt gccttggttt
    13861 gtgaagcaag ccttgaatcg tccatactgg aatagtactt ctgatcttga gaaatatatc
    13921 tttctctgtg ttcttgatgc agttagtcct gaatcttttg actgcatctt taaccttctt
    13981 gggaaggtat ttgatttcct ggagattatt gctcgggtag atcgtcttga tgagtgctgc
    14041 tgcgtaagcc tctctaacca tctgtgggtt agcattcttt ctgaaattga aaaggctaat
    14101 ctggggacct gcaggcatgc acgcgctaaa aacggactag ccttatttta acttgctatt
    14161 tctagctcta aaacaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact
    14221 agccttattt taacttgcta tttctagctc taaaaccgag acctcggtct cctgccacgg
    14281 atcatctgca caactctttt aaatcagctt tgatctatgt ggatagccga ggtggtacta
    14341 atactagtct ttgttgtcgt ccaattgcgt aatgggccgg cccatactgc aatacatgtc
    14401 ctgaaaggct tcatggccca ctacgaaatg cttttctcct acagtttatc ttacttcttc
    14461 acatcacgtg gtttccaacg tacccagtgt tcccggcttc cagcatttgc tggtagcacc
    14521 agtagaagac gcctgtcttg tgctatggtc cctgactgca catctgattc ctccaagatc
    14581 catgcatgcc tgataacttt aagttgcttc agaagaactt taagtgatct gttcgtatgt
    14641 ttaaagattc cttaagcttg gcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
    14701 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    14761 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    14821 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    14881 tattggctag agcagcttgc caacatggtg gagcacgaca ctctcgtcta ctccaagaat
    14941 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
    15001 tcgggaaacc tcctcggatt
//
