LOCUS       sequence-339897-       10098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 CMV-OsTIR1(F74G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano 
            R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, 
            Hayashi KI, Kanemaki MT
  TITLE     The auxin-inducible degron 2 technology provides sharp degradation 
            control in yeast, mammalian cells, and mice.
  JOURNAL   Nat Commun. 2020 Nov 11;11(1):5701. doi: 10.1038/s41467-020-19532-z.
  PUBMED    33177522
REFERENCE   2  (bases 1 to 10098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-11"; volume: "11(1):5701. doi"; pages: " 
            10.1038/s41467-020-19532-z"
COMMENT     Sequence Label: pMK381 (AAVS1 CMV-OsTIR1F74G)
FEATURES             Location/Qualifiers
     source          1..10098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    295..1098
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     enhancer        1153..1456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1457..1660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1611..1631
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1698..3425
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein, 
                     mutated for use in the AID2 system of inducible degradation
                     (Yesbolatova et al., 2020)"
                     /label=OsTIR1(F74G)
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     polyA_signal    3599..3720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3636..3655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3690..3709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3747..3780
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3841..4340
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3876..3899)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4273..4292
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4380..4979
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4380..4399)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    5536..5569
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5600..6436
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(6470..6489)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6471..6489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6496..6513)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6496..6512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6505..6527)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6650..6952
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6831..6850
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             7301..8095
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7301..8095
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7355..7374)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7965..7984
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(8345..9136,9137..9205))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8968..8987
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9329..9917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9818..9837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10071..10088
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa gctagtaatc aattacgggg tcattagttc atagcccata
     1141 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1201 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1261 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1321 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1381 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1441 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     1501 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     1561 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1621 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
     1681 ccgctagcgc tgccaccatg acatactttc ctgaagaggt cgtcgaacac atttttagct
     1741 tcctgcctgc acagagagat agaaacacag tgagcctggt ctgcaaagtg tggtacgaga
     1801 tcgaacgcct gagccggaga ggagtgttcg tcggcaactg ctatgctgtg agagcaggca
     1861 gggtcgccgc taggtttcca aatgtgcgcg cactgaccgt caaggggaaa ccccacggcg
     1921 ccgactttaa cctggtgccc cctgattggg gaggatacgc cggcccttgg atcgaggcag
     1981 ccgctcgcgg ctgtcatgga ctggaggaac tgcgcatgaa gcgaatggtg gtctctgacg
     2041 aaagtctgga gctgctggct cggagcttcc ctaggtttcg cgcactggtg ctgatttctt
     2101 gcgaaggctt cagcaccgat ggactggcag ccgtggcctc ccactgtaag ctgctgcggg
     2161 agctggacct ccaggagaat gaagtggagg atagaggccc cagatggctg tcttgcttcc
     2221 cagactcatg taccagcctg gtgtccctga actttgcctg catcaaaggc gaagtgaatg
     2281 ctgggtccct ggagcggctg gtctcaagaa gccccaacct gaggtctctg cggctgaacc
     2341 ggagcgtgag cgtggacact ctggctaaga ttctgctgag aacccctaac ctggaggatc
     2401 tgggaaccgg caatctgaca gacgatttcc agacagaatc ctactttaaa ctgacttctg
     2461 ccctggagaa gtgtaaaatg ctgaggagtc tgtcaggatt ctgggatgct tcacccgtgt
     2521 gcctgagctt tatctaccct ctgtgtgcac agctgacagg cctgaacctg agctatgcac
     2581 caaccctgga cgccagtgat ctgacaaaga tgatctcacg ctgcgtgaaa ctccagcgac
     2641 tgtgggtgct ggactgtatt tccgataagg ggctccaggt ggtcgccagc tcctgcaagg
     2701 acctccagga gctgagagtg ttcccatctg atttttacgt ggccggatat agtgctgtca
     2761 ctgaggaagg cctggtggca gtctcactgg gatgcccaaa gctgaacagc ctgctgtatt
     2821 tctgtcatca gatgactaat gctgcactgg tgaccgtcgc caagaactgc cctaatttca
     2881 cccgatttcg gctgtgtatt ctggaaccag gcaaacccga cgtggtcaca tcccagccac
     2941 tggatgaagg gtttggagct atcgtgagag agtgcaaggg actccagagg ctgagcattt
     3001 ccggcctgct gacagacaaa gtgttcatgt acatcggcaa gtatgctaag cagctggaga
     3061 tgctgagcat tgcatttgcc ggagactccg ataagggcat gatgcacgtg atgaacgggt
     3121 gtaagaatct gcgaaaactg gaaatccggg acagcccttt cggggatgcc gctctgctgg
     3181 gaaactttgc cagatacgag acaatgagga gcctgtggat gtctagttgc aatgtgactc
     3241 tgaagggctg tcaggtcctg gctagtaaaa tgcctatgct gaacgtggaa gtcattaatg
     3301 agcgggacgg gtctaacgaa atggaggaaa atcatggcga cctgccaaag gtggagaaac
     3361 tgtatgtgta tcggaccacc gcaggggcaa gagatgatgc tcccaacttt gtgaagattc
     3421 tgtgaggatc catcggatcc cgggcccgtc gacggtaccg cgggcccggg atccaccggg
     3481 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     3541 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3601 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3661 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3721 agcgatcata ttcaataacc cttaatataa cttcgtataa tgtatgctat acgaagttat
     3781 taggtctgaa gaggagttta cgtccagcca agcttaggat ctcgacctcg aaattctacc
     3841 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     3901 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     3961 gccaaccgac tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     4021 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     4081 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     4141 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     4201 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     4261 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     4321 cctcatctcc gggcctttcg acctgcatcc atctagatct cgagcagctg aagcttacca
     4381 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     4441 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     4501 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     4561 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     4621 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     4681 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     4741 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     4801 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     4861 agacctccgc gccccacaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     4921 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     4981 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgatga
     5041 tatcagatcc ccgggatgca gaaattgatg atctattaaa caataaagat gtccactaaa
     5101 atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac ctacattttg
     5161 aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa tgcctgctct
     5221 ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat atcataattt
     5281 aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc tatagatcta
     5341 tagatctctc gtgggatcat tgtttttctc ttgattccca ctttgtggtt ctaagtactg
     5401 tggtttccaa atgtgtcagt ttcatagcct gaagaacgag atcagcagcc tctgttccac
     5461 atacacttca ttctcagtat tgttttgcca agttctaatt ccatcagaag ctggtcgaga
     5521 tccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtccctcg
     5581 aagaggttca ctaagcttta ctagggacag gattggtgac agaaaagccc catccttagg
     5641 cctcctcctt cctagtctcc tgatattggg tctaaccccc acctcctgtt aggcagattc
     5701 cttatctggt gacacacccc catttcctgg agccatctct ctccttgcca gaacctctaa
     5761 ggtttgctta cgatggagcc agagaggatc ctgggaggga gagcttggca gggggtggga
     5821 gggaaggggg ggatgcgtga cctgcccggt tctcagtggc caccctgcgc taccctctcc
     5881 cagaacctga gctgctctga cgcggctgtc tggtgcgttt cactgatcct ggtgctgcag
     5941 cttccttaca cttcccaaga ggagaagcag tttggaaaaa caaaatcaga ataagttggt
     6001 cctgagttct aactttggct cttcaccttt ctagtcccca atttatattg ttcctccgtg
     6061 cgtcagtttt acctgtgaga taaggccagt agccagcccc gtcctggcag ggctgtggtg
     6121 aggagggggg tgtccgtgtg gaaaactccc tttgtgagaa tggtgcgtcc taggtgttca
     6181 ccaggtcgtg gccgcctcta ctccctttct ctttctccat ccttctttcc ttaaagagtc
     6241 cccagtgcta tctgggacat attcctccgc ccagagcagg gtcccgcttc cctaaggccc
     6301 tgctctgggc ttctgggttt gagtccttgg caagcccagg agaggcgctc aggcttccct
     6361 gtcccccttc ctcgtccacc atctcatgcc cctggctctc ctgccccttc cctacagggg
     6421 ttcctggctc tgctctaagg gcgaattcgc ggccgctaaa ttcaattcgc cctatagtga
     6481 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6541 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6601 ggcccgcacc gatcgccctt cccaacagtt gcgcagccta tacgtacggc agtttaaggt
     6661 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     6721 tgacacgccg gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     6781 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     6841 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     6901 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     6961 catgagatta tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca
     7021 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc
     7081 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
     7141 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg
     7201 gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg
     7261 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     7321 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     7381 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     7441 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     7501 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     7561 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     7621 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     7681 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     7741 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     7801 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     7861 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     7921 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     7981 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     8041 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat
     8101 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     8161 ccgcatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8221 aaatacattc aaatatgtat ccgctcatga gattatcaaa aaggatcttc acctagatcc
     8281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8641 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8941 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9241 agggttattg tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9361 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9421 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9481 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9541 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9901 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9961 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10021 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10081 tgagcgagga agcggaag
//
