LOCUS       sequence-339913-       11134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging 
            (the recipient vector can be TetON 3G promoter system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park 
            J, Blackburn EH, Weissman JS, Qi LS, Huang B
  TITLE     Dynamic Imaging of Genomic Loci in Living Human Cells by an 
            Optimized CRISPR/Cas System.
  JOURNAL   Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
  PUBMED    24360272
REFERENCE   2  (bases 1 to 11134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.12"; journalName: "Cell"; date: "2013-12-19- 
            19"; volume: "155"; issue: "7"; pages: "1479-91"
COMMENT     Sequence Label: pSLQ1658-dCas9-EGFP
FEATURES             Location/Qualifiers
     source          1..11134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1417..1426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1432..1452
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1453..1476,1477..1479,1480..3966,3967..3969,
                     3970..5553)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5563..5583
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5593..5595,5596..6309)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5593..5595,5596..6306)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5635..5656)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5896..5915)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6243..6264
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6320..6807
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6355..6378)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6740..6759
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6828..7427
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6828..6847)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7324..7344
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7527..8041
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8203..8219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8227..8233,8234..8251,8252..8257))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8272..8293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8410..8427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8581..9169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8661..8680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9340..10131,10132..10200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9963..9982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10201..10305)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10373..10391
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10429..10451)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10551..10570
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10764..10786
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10914..10933
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(11133..11134,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctcgcca ccatggctag ccccaaaaag
     1441 aagaggaaag tggacaagaa gtattctatc ggactggcca tcgggactaa tagcgtcggg
     1501 tgggccgtga tcactgacga gtacaaggtg ccctctaaga agttcaaggt gctcgggaac
     1561 accgaccggc attccatcaa gaaaaatctg atcggagctc tcctctttga ttcaggggag
     1621 accgctgaag caacccgcct caagcggact gctagacggc ggtacaccag gaggaagaac
     1681 cggatttgtt accttcaaga gatattctcc aacgaaatgg caaaggtcga cgacagcttc
     1741 ttccataggc tggaagaatc attcctcgtg gaagaggata agaagcatga acggcatccc
     1801 atcttcggta atatcgtcga cgaggtggcc tatcacgaga aatacccaac catctaccat
     1861 cttcgcaaaa agctggtgga ctcaaccgac aaggcagacc tccggcttat ctacctggcc
     1921 ctggcccaca tgatcaagtt cagaggccac ttcctgatcg agggcgacct caatcctgac
     1981 aatagcgatg tggataaact gttcatccag ctggtgcaga cttacaacca gctctttgaa
     2041 gagaacccca tcaatgcaag cggagtcgat gccaaggcca ttctgtcagc ccggctgtca
     2101 aagagccgca gacttgagaa tcttatcgct cagctgccgg gtgaaaagaa aaatggactg
     2161 ttcgggaacc tgattgctct ttcacttggg ctgactccca atttcaagtc taatttcgac
     2221 ctggcagagg atgccaagct gcaactgtcc aaggacacct atgatgacga tctcgacaac
     2281 ctcctggccc agatcggtga ccaatacgcc gaccttttcc ttgctgctaa gaatctttct
     2341 gacgccatcc tgctgtctga cattctccgc gtgaacactg aaatcaccaa ggcccctctt
     2401 tcagcttcaa tgattaagcg gtatgatgag caccaccagg acctgaccct gcttaaggca
     2461 ctcgtccggc agcagcttcc ggagaagtac aaggaaatct tctttgacca gtcaaagaat
     2521 ggatacgccg gctacatcga cggaggtgcc tcccaagagg aattttataa gtttatcaaa
     2581 cctatccttg agaagatgga cggcaccgaa gagctcctcg tgaaactgaa tcgggaggat
     2641 ctgctgcgga agcagcgcac tttcgacaat gggagcattc cccaccagat ccatcttggg
     2701 gagcttcacg ccatccttcg gcgccaagag gacttctacc cctttcttaa ggacaacagg
     2761 gagaagattg agaaaattct cactttccgc atcccctact acgtgggacc cctcgccaga
     2821 ggaaatagcc ggtttgcttg gatgaccaga aagtcagaag aaactatcac tccctggaac
     2881 ttcgaagagg tggtggacaa gggagccagc gctcagtcat tcatcgaacg gatgactaac
     2941 ttcgataaga acctccccaa tgagaaggtc ctgccgaaac attccctgct ctacgagtac
     3001 tttaccgtgt acaacgagct gaccaaggtg aaatatgtca ccgaagggat gaggaagccc
     3061 gcattcctgt caggcgaaca aaagaaggca attgtggacc ttctgttcaa gaccaataga
     3121 aaggtgaccg tgaagcagct gaaggaggac tatttcaaga aaattgaatg cttcgactct
     3181 gtggagatta gcggggtcga agatcggttc aacgcaagcc tgggtaccta ccatgatctg
     3241 cttaagatca tcaaggacaa ggattttctg gacaatgagg agaacgagga catccttgag
     3301 gacattgtcc tgactctcac tctgttcgag gaccgggaaa tgatcgagga gaggcttaag
     3361 acctacgccc atctgttcga cgataaagtg atgaagcaac ttaaacggag aagatatacc
     3421 ggatggggac gccttagccg caaactcatc aacggaatcc gggacaaaca gagcggaaag
     3481 accattcttg atttccttaa gagcgacgga ttcgctaatc gcaacttcat gcaacttatc
     3541 catgatgatt ccctgacctt taaggaggac atccagaagg cccaagtgtc tggacaaggt
     3601 gactcactgc acgagcatat cgcaaatctg gctggttcac ccgctattaa gaagggtatt
     3661 ctccagaccg tgaaagtcgt ggacgagctg gtcaaggtga tgggtcgcca taaaccagag
     3721 aacattgtca tcgagatggc cagggaaaac cagactaccc agaagggaca gaagaacagc
     3781 agggagcgga tgaaaagaat tgaggaaggg attaaggagc tcgggtcaca gatccttaaa
     3841 gagcacccgg tggaaaacac ccagcttcag aatgagaagc tctatctgta ctaccttcaa
     3901 aatggacgcg atatgtatgt ggaccaagag cttgatatca acaggctctc agactacgac
     3961 gtggacgcca tcgtccctca gagcttcctc aaagacgact caattgacaa taaggtgctg
     4021 actcgctcag acaagaaccg gggaaagtca gataacgtgc cctcagagga agtcgtgaaa
     4081 aagatgaaga actattggcg ccagcttctg aacgcaaagc tgatcactca gcggaagttc
     4141 gacaatctca ctaaggctga gaggggcgga ctgagcgaac tggacaaagc aggattcatt
     4201 aaacggcaac ttgtggagac tcggcagatt actaaacatg tcgcccaaat ccttgactca
     4261 cgcatgaata ccaagtacga cgaaaacgac aaacttatcc gcgaggtgaa ggtgattacc
     4321 ctgaagtcca agctggtcag cgatttcaga aaggactttc aattctacaa agtgcgggag
     4381 atcaataact atcatcatgc tcatgacgca tatctgaatg ccgtggtggg aaccgccctg
     4441 atcaagaagt acccaaagct ggaaagcgag ttcgtgtacg gagactacaa ggtctacgac
     4501 gtgcgcaaga tgattgccaa atctgagcag gagatcggaa aggccaccgc aaagtacttc
     4561 ttctacagca acatcatgaa tttcttcaag accgaaatca cccttgcaaa cggtgagatc
     4621 cggaagaggc cgctcatcga gactaatggg gagactggcg aaatcgtgtg ggacaagggc
     4681 agagatttcg ctaccgtgcg caaagtgctt tctatgcctc aagtgaacat cgtgaagaaa
     4741 accgaggtgc aaaccggagg cttttctaag gaatcaatcc tccccaagcg caactccgac
     4801 aagctcattg caaggaagaa ggattgggac cctaagaagt acggcggatt cgattcacca
     4861 actgtggctt attctgtcct ggtcgtggct aaggtggaaa aaggaaagtc taagaagctc
     4921 aagagcgtga aggaactgct gggtatcacc attatggagc gcagctcctt cgagaagaac
     4981 ccaattgact ttctcgaagc caaaggttac aaggaagtca agaaggacct tatcatcaag
     5041 ctcccaaagt atagcctgtt cgaactggag aatgggcgga agcggatgct cgcctccgct
     5101 ggcgaacttc agaagggtaa tgagctggct ctcccctcca agtacgtgaa tttcctctac
     5161 cttgcaagcc attacgagaa gctgaagggg agccccgagg acaacgagca aaagcaactg
     5221 tttgtggagc agcataagca ttatctggac gagatcattg agcagatttc cgagttttct
     5281 aaacgcgtca ttctcgctga tgccaacctc gataaagtcc ttagcgcata caataagcac
     5341 agagacaaac caattcggga gcaggctgag aatatcatcc acctgttcac cctcaccaat
     5401 cttggtgccc ctgccgcatt caagtacttc gacaccacca tcgaccggaa acgctatacc
     5461 tccaccaaag aagtgctgga cgccaccctc atccaccaga gcatcaccgg actttacgaa
     5521 actcggattg acctctcaca gctcggaggg gatgagggag ctcccaagaa aaagcgcaag
     5581 gtaggtagtt ccgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     5641 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     5701 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     5761 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     5821 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     5881 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     5941 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     6001 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     6061 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     6121 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     6181 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     6241 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     6301 tacaagtaag aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     6361 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     6421 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     6481 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     6541 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     6601 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     6661 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcggggc gggcgcccga
     6721 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     6781 tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca
     6841 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     6901 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6961 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     7021 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     7081 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     7141 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     7201 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     7261 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     7321 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     7381 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     7441 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgataaaa taaaagattt
     7501 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     7561 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     7621 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     7681 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     7741 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     7801 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     7861 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
     7921 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     7981 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     8041 tgggtaacag tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag
     8101 taatcctgac tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt
     8161 tcattatgga cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat
     8221 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8281 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8341 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8401 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8461 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8521 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8581 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8641 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8701 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8761 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8821 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8881 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8941 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9001 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9061 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9121 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9181 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9241 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9301 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9361 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9421 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9481 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9541 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9601 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9661 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9721 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9781 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9841 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9901 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9961 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10021 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10081 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10141 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10201 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10261 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10321 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    10381 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    10441 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10501 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    10561 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    10621 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    10681 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    10741 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat
    10801 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
    10861 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
    10921 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
    10981 ccggcgtaga ggcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta
    11041 gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa
    11101 aagattttat ttagtctcca gaaaaagggg ggaa
//
