LOCUS       sequence-339956-        2227 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lactate biosensor in anaerobic conditions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Zuniga A, Camacho M, Chang HJ, Fristot E, Mayonove P, Hani EH, 
            Bonnet J
  TITLE     Engineered l-Lactate Responding Promoter System Operating in 
            Glucose-Rich and Anoxic Environments.
  JOURNAL   ACS Synth Biol. 2021 Dec 1. doi: 10.1021/acssynbio.1c00456.
  PUBMED    34851606
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Dec 1. doi: 10.1021/acssynbio.1c00456."
COMMENT     Sequence Label: ALPaGA
FEATURES             Location/Qualifiers
     source          1..2227
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1198..1209
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(1216..1218,1219..1932)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2027..2098
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2114..2141
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctcggatacc cttactctgt tgaaaacgaa tagataggtt tcatgcgttt ttctccctcg
       61 aatgctcatt atgctcaccg ggcaggcggg taatccgtgc gtggcgagcc tgatcttcat
      121 cgaatcgttt catggtggtg tgaacaaaac taaggtgcgc catcattgct ttacgcgccc
      181 cgtcagcatc accggcaaaa atggcgtcaa tgacagcctg atgttgttcg gtcagttgtg
      241 aaaaaaccgg tggcaccaga tacatccgct gacggctatg cttcactgag gattgcagga
      301 catcgaagaa accgcgcatg gtttgcagca gcacgatgtt atgtgaggct tcggcaatcg
      361 ccagatgaaa acgaacgtcc gcttgtgagg cgatatccgg gtcttcactt agcgttgctt
      421 caaagcaaag ctgaatcttt tctttgtcgc caggtgtggc gcgcattgcc gcatgccatg
      481 cggtgctggc ttcaatggcg tagcgggctt ccagaatatc gaaactgtaa tccggatcat
      541 cggccatcag tgtttttagc ggctggacga tgttttgctc cgaccatgtg tcatgacgcc
      601 agcgaataaa cgtcccgccg ccgcgtcgac tgagcagcac gccttcactc accagttttg
      661 ccagcgcctc gcgcagtgaa ttacgtgata cgccgagttg catcgccagt tggcgctcag
      721 cgggcaactt catgcccgct tccaggtttt tttcatcaat cagcgcccgc acacgatcgg
      781 caacctcgtc tgacaggcgt ctgggtaaaa caatcatcta ctagtgggtc ctgtctttct
      841 ctagatccac acattatacg agccgatgat taagaggcaa agattactac tgataaccac
      901 tgttgattgg gatacccgta agagaatata aaaagccaga ttattaatcc ggctttttta
      961 ttatttcttt accagacatc tccccccaca agaattggcc ctaccaattc ttcgcttaaa
     1021 aggtcccgtc tgaacttacg tcgcttttaa ctattcttaa acttgacaat taatcatccg
     1081 gctcttagtg tttgtggagt cattatccct acacaacaca attggcagtg ccacttttac
     1141 acaacgtgtg acaaggagat gagcaacaga ctcattacac gatgtgcgtg gactccaaaa
     1201 gaggagaaat actagatgtc aaaaggagaa gaacttttta caggtgtagt acctatcttg
     1261 gttgaattgg atggtgatgt taacggtcac aaattttctg tacgtggtga aggtgaaggt
     1321 gatgcaacta acggtaaatt gacacttaaa ttcatttgta caactggaaa acttcctgtt
     1381 ccttggccta ctcttgttac aacattgaca tatggagtac aatgtttttc acgttatcct
     1441 gatcatatga aacgtcacga tttttttaaa tctgctatgc cagaaggtta tgtacaagaa
     1501 cgtacaattt catttaaaga tgacggaaca tataaaacac gtgctgaagt aaaattcgaa
     1561 ggtgacactc ttgttaatcg tatcgaattg aaaggaatcg atttcaaaga agatggtaac
     1621 attttgggac acaaacttga atacaacttc aactctcata atgtttatat cacagctgac
     1681 aaacaaaaaa acggtattaa agctaatttt aaaattcgtc acaatgttga agatggatct
     1741 gttcaattgg ctgatcatta tcaacaaaat acaccaatcg gagacggacc agtattgctt
     1801 ccagataacc actacctttc tactcaatca gttctttcaa aagatcctaa cgaaaaacgt
     1861 gaccatatgg tacttcttga atttgttaca gcagcaggta tcactcacgg tatggacgaa
     1921 ctttataaat aaactttatc tgagaatagt caatcttcgg aaatcccagg tggcatgcta
     1981 aaagtctcgt aaagcgttct atcaataacc cgttggtgcc aggcatcaaa taaaacgaaa
     2041 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2101 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttataccg tctcagaatc
     2161 ggccgtgaac aataaaatag tttcggtatt attgaccact tccgagtaga atcgtgcttc
     2221 agtaaga
//
