LOCUS       sequence-339964-        6290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CCR6 receiver construct in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Cho KF, Gillespie SM, Kalogriopoulos NA, Quezada MA, Jacko M, Monje 
            M, Ting AY
  TITLE     A light-gated transcriptional recorder for detecting cell-cell 
            contacts.
  JOURNAL   Elife. 2022 Mar 21;11:e70881. doi: 10.7554/eLife.70881.
  PUBMED    35311648
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-21"; volume: "11:e70881. doi"; pages: " 
            10.7554/eLife.70881"
COMMENT     Sequence Label: pAAV CMV CCR6-eLOV-TEVcs-Gal4
FEATURES             Location/Qualifiers
     source          1..6290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        201..504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        505..708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     659..679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     753..772
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        753..771
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2396..2416
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             2429..2452
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2453..2890
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2891..3232
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             3233..3274
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3311..3328)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3317..3524
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3553..3693
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3553..3682
                     /label=AAV2 ITR
     rep_origin      3768..4223
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3855..3874)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4065..4086
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4240..4259)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4359..4381
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4419..4437)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4505..4609
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4610..4678,4679..5470)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4828..4847)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5641..6229
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6130..6149
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca tagtaatcaa ttacggggtc attagttcat
      181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      301 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      721 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      781 gcttgccacc atgagcggag agagcatgaa cttcagtgat gtgttcgatt ccagtgagga
      841 ctacttcgtg tccgtcaata catcctacta cagcgtagat tctgagatgc tgctttgctc
      901 cctgcaggag gtgcgccagt tctcccgact cttcgttccc atcgcgtatt cactcatctg
      961 cgtctttggc ctcctgggga atatcttggt agtcatcaca tttgcttttt ataaaaaggc
     1021 cagatccatg acagacgtct acctgcttaa tatggccatt gcagacatcc tgttcgtcct
     1081 gacgctccca ttctgggctg tgagtcacgc taccggggcc tgggtgttca gtaatgcaac
     1141 ctgcaagctg ttgaagggaa tttatgccat caactttaat tgcggtatgc tgctgctgac
     1201 ttgcataagt atggacagat acatcgccat agtacaggca acaaaatcct tcagactgag
     1261 gtctcgaacg ctccctaggt ctaaaatcat ttgtcttgtt gtatggggct tgagcgtgat
     1321 tatttccagc agtactttcg tgtttaacca gaagtacaac acgcagggca gcgatgtgtg
     1381 tgagcccaag taccagacgg tatctgagcc tatcaggtgg aagctgttga tgctgggttt
     1441 ggaattgctg ttcggctttt ttatcccact catgtttatg attttttgtt ataccttcat
     1501 cgtgaagacc ctggtccagg cacaaaactc taagcgacac aaggccatcc gggtaatcat
     1561 cgctgttgtg ctggtatttc tggcttgcca gatccctcat aatatggtcc ttctggtaac
     1621 ggcagccaac ctgggcaaaa tgaatcgctc atgtcagtca gaaaagctta tcgggtacac
     1681 caaaacggtt acggaggttc tggcgttcct gcattgttgc cttaaccctg tgctgtacgc
     1741 atttataggt cagaagtttc gaaactactt cctgaagatt ctcaaagacc tctggtgtgt
     1801 aagaagaaag tataaaagta gtggattttc atgcgcagga cgctactccg aaaacatttc
     1861 cagacagacc agtgagacgg ccgataacga taacgcctcc tcctttacca tgactagtga
     1921 gctggccgag aagctggccg gcctggacat caacggtgga gctagcggca gccgggcgac
     1981 caccctggaa cgtattgaaa agagctttgt gattaccgat ccgcgtctgc cggataaccc
     2041 gattattttt gtgagcgata gctttctgca gctgaccgaa tatagccgtg aagaaattct
     2101 gggccgtaac tgccgttttc tgcagggccc ggaaaccgat cgtgcgaccg tgcgtaaaat
     2161 tcgtgatgcg attgataacc agaccgaagt gaccgtgcag ctgattaact ataccaaaag
     2221 cggcaaaaaa ttttggaacc tgtttcatct gcagccgatg cgtgatcaga aaggcgatgt
     2281 gcagtatttt attggcgtgc agctggatgg taccgaacgt gtgcgtgatg cggcggaacg
     2341 tgaagctgtg atgctggtca agaaaaccgc ggaggaaatc gacgaagccg ctaaggaaaa
     2401 tctctacttc cagatgggcg gaggatccga ctacaaggat gacgacgata agaagctact
     2461 gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga
     2521 aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac
     2581 caaaaggtct ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact
     2641 ggaacagcta tttctactga tttttcctcg agaagacctt gacatgattt tgaaaatgga
     2701 ttctttacag gatataaaag cattgttaac aggattattt gtacaagata atgtgaataa
     2761 agatgccgtc acagatagat tggcttcagt ggagactgat atgcctctaa cattgagaca
     2821 gcatagaata agtgcgacat catcatcgga agagagtagt aacaaaggtc aaagacagtt
     2881 gactgtatcg gccaatttta atcaaagtgg gaatattgct gatagctcat tgtccttcac
     2941 tttcactaac agtagcaacg gtccgaacct cataacaact caaacaaatt ctcaagcgct
     3001 ttcacaacca attgcctcct ctaacgttca tgataacttc atgaataatg aaatcacggc
     3061 tagtaaaatt gatgatggta ataattcaaa accactgtca cctggttgga cggaccaaac
     3121 tgcgtataac gcgtttggaa tcactacagg gatgtttaat accactacaa tggatgatgt
     3181 atataactat ctattcgatg atgaagatac cccaccaaac ccaaaaaaag agggcaagcc
     3241 gatccctaac cctctgctgg gcctggacag cacttagtaa gaattcctcg agctagagct
     3301 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3361 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3421 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3481 agcaaggggg aggattggga agacaatagc aggcatgctg gggaggtaac cacgtgcgga
     3541 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     3601 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     3661 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     3721 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     3781 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3841 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3901 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3961 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     4021 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4081 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     4141 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4201 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     4261 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4321 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4381 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     4441 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4501 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4561 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4621 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4681 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4741 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4801 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4861 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4921 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4981 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5041 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5101 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5161 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5221 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5281 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat
     5341 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5401 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5461 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5521 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5581 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5641 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5701 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5761 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5821 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5881 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5941 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6001 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6061 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6121 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6181 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6241 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
