LOCUS       sequence-339970-        9070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of codon-optimized His-SUMO-LbuCas13a..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Liu TY, Knott GJ, Smock DCJ, Desmarais JJ, Son S, Bhuiya A, 
            Jakhanwal S, Prywes N, Agrawal S, Diaz de Leon Derby M, Switz NA, 
            Armstrong M, Harris AR, Charles EJ, Thornton BW, Fozouni P, Shu J, 
            Stephens SI, Kumar GR, Zhao C, Mok A, Iavarone AT, Escajeda AM, 
            McIntosh R, Kim S, Dugan EJ, Pollard KS, Tan MX, Ott M, Fletcher DA,
            Lareau LF, Hsu PD, Savage DF, Doudna JA
  TITLE     Accelerated RNA detection using tandem CRISPR nucleases.
  JOURNAL   Nat Chem Biol. 2021 Sep;17(9):982-988. doi: 
            10.1038/s41589-021-00842-2. Epub 2021 Aug 5.
  PUBMED    34354262
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00842-2"; journalName: "Nat Chem Biol"; date: 
            "2021-09"; volume: "17"; issue: "9"; pages: "982-988"
COMMENT     Sequence Label: pGJK_His-SUMO-LbuCas13a
FEATURES             Location/Qualifiers
     source          1..9070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     975..994
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1014..1091)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1297..1316
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1400..1419
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1400..1418
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1419..1443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1458..1480
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1499..1516
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1526..1543
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5330..5347
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5400..5418)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5414..5461
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5498..5953
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5585..5604)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5795..5816
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6046..6861)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6046..6861)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6769..6788
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6853..6872
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6983..7571
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7472..7491
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7725..7742
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7757..7899
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7825..7844)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7985..8007
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8001..8192)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9001..9070,1..1013))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
       61 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
      121 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
      181 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
      241 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
      301 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
      361 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
      421 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
      481 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
      541 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
      601 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
      661 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
      721 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
      781 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
      841 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
      901 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
      961 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     1021 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
     1081 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     1141 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     1201 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     1261 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     1321 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
     1381 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
     1441 tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca
     1501 tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgt caggtagtgc
     1561 agctggggga gaagaggaca aaaaaccggc aggtggcgag ggtggcggtg cacatattaa
     1621 tttaaaggtg aaaggtcagg atggtaatga agtcttcttc cgaatcaaac gcagcaccca
     1681 gctgaaaaaa ctgatgaatg cgtactgcga tcgtcagtcc gttgatatga ccgcaattgc
     1741 gttcttgttc gacggccgcc gtctgcgtgc tgaacagact ccggatgaat tggagatgga
     1801 agacggcgat gagatcgacg caatgctgca ccagaccggt ggcatgaaag tcaccaaagt
     1861 gggtggtatc tcccataaaa aatacacctc tgagggtcgt ctggttaaga gtgagtccga
     1921 ggaaaaccgt acggatgagc gcctgtccgc tctgttaaat atgcgtctgg atatgtatat
     1981 caagaacccg agctccaccg aaacgaaaga aaatcagaaa cgtattggca agctgaaaaa
     2041 attcttctct aacaagatgg tatatttgaa ggataatacc ctgagcctga agaatggtaa
     2101 gaaggagaac atagaccgcg aatactcaga gacagatatc ctggaatcag acgtgcgtga
     2161 taagaagaac ttcgccgttc tgaagaaaat ctatctaaac gaaaacgtta attccgaaga
     2221 attggaggtc tttcgtaacg acatcaaaaa gaagctcaac aaaatcaact cgctgaagta
     2281 cagctttgaa aagaataagg cgaattatca aaaaatcaac gaaaacaaca tcgaaaaggt
     2341 ggagggcaaa tcgaaacgca acatcattta cgactattat cgtgaaagcg cgaaacgtga
     2401 tgcgtacgtt agcaatgtga aagaagcctt cgacaaatta tataaagagg aggatatcgc
     2461 taaattagtt ctggagatcg aaaacctgac caaattggag aaatacaaga ttcgtgaatt
     2521 ttaccatgaa attatcggcc gtaagaacga taaggagaat tttgcgaaaa tcatctatga
     2581 ggagattcag aatgttaata acatgaaaga actcatcgaa aaggttccgg atatgtctga
     2641 gctgaagaaa tctcaggttt tttacaagta ctacctggac aaagaagagc ttaatgacaa
     2701 gaacatcaaa tatgcattct gccattttgt tgaaatcgaa atgagccagc tgctgaagaa
     2761 ttacgtgtat aagagactct ctaacattag caacgacaaa atcaagcgta ttttcgagta
     2821 tcaaaacttg aagaagctga tagaaaacaa gctgctcaac aaactggaca cctatgtgcg
     2881 taattgtggt aaatacaatt actatctgca agacggtgag attgcgacca gcgacttcat
     2941 cgcgcgtaac cgccaaaacg aggcgtttct gcgtaatatc atcggagtta gcagcgttgc
     3001 atatttcagc ctacgcaaca tcctggaaac cgaaaacgag aatgatatca ccggtcgcat
     3061 gcgtggtaag accgttaaaa ataacaaggg cgaggaaaag tacgtgagcg gtgaagtgga
     3121 taaaatttat aacgagaata aaaagaatga ggtgaaagaa aacctgaaga tgttttacag
     3181 ctatgacttc aacatggata ataaaaacga aattgaagat ttcttcgcca acatcgatga
     3241 ggccatttcc tctattcgtc atggtatagt acacttcaac ctggagctgg aaggcaagga
     3301 catctttgcc ttcaaaaaca tcgctccgag cgaaatctcc aaaaaaatgt ttcagaacga
     3361 gatcaatgag aaaaagttga agctgaaaat ctttcgccaa ctgaattctg cgaatgtctt
     3421 ccgttatctg gagaagtaca agattctgaa ttacttgaaa cgcacccgtt ttgagttcgt
     3481 caacaagaac atcccatttg tgccgtcgtt caccaaattg tacagccgta ttgacgacct
     3541 gaaaaactcc ctgggcatct actggaaaac cccgaaaacc aatgacgata acaagactaa
     3601 agagatcatc gatgctcaga tttacctgct gaagaacata tattatggcg agttccttaa
     3661 ctatttcatg agcaataacg gcaatttttt cgagatttct aaagagatta ttgaactgaa
     3721 caagaatgat aagcgcaact tgaagaccgg cttttataag ctacaaaaat ttgaggacat
     3781 tcaagaaaaa attccgaaag aatacttggc gaacatccag agtctgtaca tgattaatgc
     3841 aggcaatcag gatgaggagg aaaaagacac ctatatcgac tttattcaaa aaattttcct
     3901 gaaaggcttc atgacgtact tagcgaataa tggtcgcctt agcctgattt acatcggctc
     3961 cgatgaggaa acgaatacca gtctggctga aaagaagcag gagttcgata aatttttgaa
     4021 gaaatatgaa caaaacaaca acattaagat tccgtatgaa atcaacgagt ttctgaggga
     4081 aatcaaactt ggtaacattt tgaagtacac tgagcgcctg aacatgtttt acctgattct
     4141 gaagttgctt aaccacaaag agttgaccaa tctgaaaggc agcctggaga agtaccaatc
     4201 ggcgaacaag gaagaggcgt tcagcgacca gctggagttg atcaacctgc tgaacctgga
     4261 caacaaccgt gttaccgaag actttgagct cgaagcggat gaaattggta aattcctgga
     4321 ctttaatggt aacaaggtga aggacaacaa agagctgaag aagtttgata ccaacaaaat
     4381 ctacttcgac ggcgaaaaca tcatcaagca ccgtgccttc tacaacatta aaaagtacgg
     4441 tatgctgaat ctgctggaaa aaatcgccga taaggcgggt tataaaatct cgatcgaaga
     4501 gttgaaaaaa tatagcaaca agaagaatga gattgagaaa aaccacaaaa tgcaggagaa
     4561 cttgcaccgc aagtacgctc gccctcgtaa ggacgaaaaa tttaccgacg aggactacga
     4621 gagctacaag caggcaattg agaacattga ggagtatacg catctcaaaa acaaggttga
     4681 attcaacgag ttaaatctgc tgcaaggtct gttgctgcgc attcttcacc gtttagttgg
     4741 ttatacgagc atttgggaaa gagacctgcg ttttcgtctg aagggtgagt tcccggaaaa
     4801 ccagtatatt gaagaaattt ttaatttcga gaacaagaaa aacgtaaaat acaagggtgg
     4861 ccaaattgtt gagaagtaca tcaagttcta taaggagctt caccaaaacg acgaggtgaa
     4921 aataaacaaa tactcctctg cgaatattaa agtgctgaag caagaaaaga aagatctgta
     4981 cattcgcaac tacattgccc actttaacta tatcccgcat gcggaaattt ccctgttgga
     5041 agtgctggaa aacctgcgta agctgctgag ctacgatcgt aaactcaaga atgcggttat
     5101 gaagtccgtg gtggacattc tgaaggagta tggttttgta gcgaccttta aaatcggcgc
     5161 ggataagaaa atcggcattc aaaccctgga atccgaaaaa attgtccacc tgaagaacct
     5221 gaagaagaag aaactgatga cggatcgtaa tagcgaggag ctttgcaaac tggtgaaaat
     5281 catgttcgag tacaaaatgg aagagaagaa aagcgagaat taactcgagc accaccacca
     5341 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     5401 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     5461 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
     5521 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5581 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5641 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5701 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5761 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5821 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5881 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5941 acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6001 tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga aaaactcatc
     6061 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     6121 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     6181 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     6241 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     6301 tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     6361 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     6421 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     6481 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     6541 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     6601 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     6661 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     6721 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     6781 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt
     6841 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     6901 tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6961 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7021 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7081 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7141 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7201 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7261 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7321 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7381 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7441 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7501 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7561 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7621 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7681 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7741 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     7801 accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     7861 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     7921 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7981 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     8041 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     8101 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     8161 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     8221 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     8281 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     8341 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     8401 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     8461 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     8521 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     8581 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     8641 acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
     8701 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
     8761 gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
     8821 cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
     8881 gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
     8941 ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
     9001 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9061 cgcgcgggga
//
