LOCUS       sequence-339972-       13752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  alphaA-crystallin promoter (cry) drives GFP expression in the lens. 
            The 600bp unc-45b muscle-specific promoter drives expression of 
            GFP-tagged zebrafish Myo18b..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13752)
  AUTHORS   Berger J, Berger S, Li M, Currie PD
  TITLE     Myo18b is essential for sarcomere assembly in fast skeletal muscle.
  JOURNAL   Hum Mol Genet. 2017 Mar 15;26(6):1146-1156. doi: 10.1093/hmg/ddx025.
  PUBMED    28104788
REFERENCE   2  (bases 1 to 13752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Hum Mol Genet"; date: "2017-03-15- 15"; volume: "26"; issue: "6"; 
            pages: "1146-1156"
COMMENT     Sequence Label: pcryBFP_Umyo18bGFP
FEATURES             Location/Qualifiers
     source          1..13752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    1057..1178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1301..2014,2015..2017,2018..2020))
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     regulatory      2017..2026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2667..2683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2703..2723
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             join(9648..9650,9651..9653,9654..10364)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQFADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(10426..10450)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    10452..10586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10649..10669)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10677..10693)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    10709..10843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(11282..11469,11470..11481)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11515..11531)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11515..11531)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11568..11588)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11568..11586)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11607..11623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11620..11642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11631..11647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11655..11661,11662..11679,11680..11685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11700..11721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11838..11855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12009..12597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12089..12108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12768..13559,13560..13628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13391..13410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13629..13733)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggcccattt acgccttaag atacattgat gagtttggac
     1081 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1141 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1201 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1261 aatgtggtat ggctgattat gatctagagt cgcggccgct ttacttgtac agctcgtcca
     1321 tgccgagagt gatcccggcg gcggtgcgga actccagcag gaccatgtga tcgcgcttct
     1381 cgttggggtc tttgctcagc acggactggg tgctcaggta gtggctgtcg ggcagcagca
     1441 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     1501 cctccacgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgacggcca
     1561 tgatatagat gttgtggctg ttgaagttgt actccagctt gtgccccagg atgttgccgt
     1621 cctccttgaa gtcgacgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     1681 tcacctcggc gcgggtcttg taggtgccgt cgtccttgaa gaagatggtg cgctcctgga
     1741 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     1801 gggcgaagca ctgcacgccg tggctcaggg tggtcacgag ggtgggccag ggcacgggca
     1861 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gttggtggca tcgccctcgc
     1921 cctcgcccct cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     1981 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc ggtaatgtca
     2041 gacctggtaa ctccttactg taacttattg cacctgatga acaaaagatg tgaactgaac
     2101 gcaagtagga ggctaataga ggctagtaca gtacaaagat ccccaaatga tgtgtgccgg
     2161 ccttagtgga ggcagacccc tggttatata cctgtgctgc agaaagaggg gcttacaaat
     2221 ggatctctct ggaatggcat tatctcatgg tgggaacagt gttgtcagca gaatcagcgg
     2281 ggtggactct gacctgagaa tgacagtcta gcactgaatg gctcagacaa tactcttgtg
     2341 gccaactatc accggaaaat aagtgcctgc caaaacaaac cagaccagat gaagctacat
     2401 ggctgtagaa cgtcagtatt taatgtgttg tggccacttg gttcaccaga tttaagagct
     2461 aattttttta aatatcacag ataatcaatc gctttttttt tcttgactga aaagcttaga
     2521 ctggaaaatt ctttgaaaaa gtttgcacta cagaaaataa atacataaca tcctgcactg
     2581 aatgcacact attaatagaa aagagagcat tattgtagga aatgtattgt atatgtacat
     2641 tctgtgcatt tcacaatttt ctcgagcagg aaacagctat gaccatgatt acgccaagct
     2701 atcaactttg tatagaaaag ttgccattat cgtctccctc cagcaccacc cagaaagttg
     2761 ctcaatgttt tgttacagta tgtgtgtaac accttccccc ccagcccagc tctccacttt
     2821 tctcattcta gaaagtacca gtcaactctc caccagccca gctgttggca gacgcacacc
     2881 tccatccccc tgccctcaga catttgccac tgatttctca gctgtcatcc cctctccata
     2941 aatagaccct atcagagaaa gtccattgca ctaatataag gggtgaccac atttctacaa
     3001 aaccacaatt aatgtacaat cacaggaata aacaaacact gcctaaacac acaatatcaa
     3061 gaacacacaa ttacaatctc aggttgtcat tcgcaagctg cgtagttcat tcacaatgaa
     3121 caggggctta aaacaaaagt cattccaccc ctcaccatga aatggattca tccgtaatcc
     3181 tggtatgtta acgctctatg tgtaagatat atataagagt gactcttaaa gtgctgctac
     3241 tactattgaa caaaccaaac gacaagaaac aacactgaag cacagaacag attttctctt
     3301 acgacgaagc tgaattgact tcaatggctc tctcctcgcg cttaaagctc tgggagcaga
     3361 agataaagga ggagaagaaa gctccagagg ccgccgctca gcctcctcct ccactgtctg
     3421 ctgttcctgg aggattcctc aagcagctgg tccgagaaac cgagaaggag acgcgacacc
     3481 gagagcccga aatcaaggaa gacaaaccgc ccagcaaact cagcgacaac ctagtccaga
     3541 atttccttct tcccgatgaa actccgccga ttctggaggc cgagatgtcg ctgcgagcgg
     3601 agcagccggt gaacggggag cgcggacggc acatcgctat gtcagagaag aagacgtctt
     3661 cacccaggag gatcatctca cccgaaaacc ccaaaagccc cagagcagaa acacagacct
     3721 gcagtcctga ggtcagtcca gacacacaca caccgcagaa ggaggagcag gtgaagatgc
     3781 tgcaggaagc ccaacccccg aaacaggaag tcaagggcgt aagacaggag ccggagggtc
     3841 agatcactgt tactcatatg aaggaggaga gcaaggtgcc tgtgaaggac gtgtggtatg
     3901 aagctggaca ggtgtggttc actcacaaag acgggtacac actcgccact cagctgaagc
     3961 cggacgaggg cacgccggag ctccccgacg gccgcgtgag ggttcgactc tcctctgatc
     4021 agtccattca tgatgtgtcc cagtatgaga tcgaaaagct gaatccacct gagctggacc
     4081 tgtgtgagga cctgagtgac cttgttagtg tgaatgagtc gagtgcactt cacactttga
     4141 gcagtagagc taaagctcat ctaccgctga cccacgccgg acccaatcta ctggcccttt
     4201 ggcctccagt gtcttcatcc accaagagtt ttcggagccg gcgctgggag tcctgggaag
     4261 cccctggccc gctgcaggct gtggtgagga aggtgtacgt gtccatgctg ggtcagcgga
     4321 gggaccagac actggtggct ttgggtcgca gcggctcagg gaaaaccacc gcctgtcaat
     4381 cattctccct ggagctgctc aaacaagcgg gaacagcagg aggaagcctg accttagagc
     4441 gtctgcaggc agtgtttaca gtgctgcgtt cgttcggttg tgtgagctca actcacagtg
     4501 aggcctcgtc tcgattcgcc atggtgctgt ctgtggactt caaccacgct ggactcgctg
     4561 ctgccgcaca tctgcagacg atgctgcttg agaagtggag ggtttgccag cggccggatg
     4621 gagagagtaa cttcctggtt ttctcccaga tgctctcagg cctgagctca gatctgagga
     4681 ctgagctgca gctgcatcag ttaaaggaac acaacttttt tggaatcaca tgtccgacca
     4741 aggtggatga gaagcagaga gccgctgtgg gtttcgggaa gctgctggcc gctatgaaca
     4801 ctttgggctt ctcatcggat gagcagaagg ctatttggcg tgtgttagca ggaatatacc
     4861 atctcggcgt cgcaggaaca tgcaaagtgg gccgtaagca gtttatgagt tttgacagcg
     4921 cgcagacggc cagtgcaatg ctgggctgtg aaggagagga gcttcacacc gccgtcttca
     4981 aacaccacct gagacagtta ctacagagag ccagcggggg catcagagag cggcagactg
     5041 cagaacaaca cgacgatggt ccgaagttga ctgcagctca gtgtgtggaa ggaatggctg
     5101 cgggtctcta tgaggagctc ttcaccgctg tggtgtctct gattaaccgc acgctgtgct
     5161 ctcagcagct cactctggcc tcagtgatgg tggtggacgc gccgggcctc aggaacccca
     5221 gacacagcgc tgaggaacgg gccgccggct tctctgagct ctgccacaac tacctgcagg
     5281 agagactgct ggagcatcac tacacacaca ccttcacaca cacactggac agatacacac
     5341 aggaaggagt gcctgtggat ttctcagccc cggagtcgag tccgtctgag gtggtgtgtg
     5401 ccatcgacca accaggactg caggttcgag ggcctgaagc tgactctcgg ggtctgctgt
     5461 gggttctgga cgaagagttg tcgacagcgg gctccagtga gagtgtggtt ctagagagag
     5521 tgtgtcagta cttcagcagc acagtgcgtc agtgtgagca gccgctgcag tgtgagatcg
     5581 ctcatctgat gggttcagat gcggtgcgct atgatctgtc cggctggttc ggtctgctcc
     5641 agaataaccc gtctctgctc aacgccagct ctctgctgca caactcctca catgtgtcgg
     5701 tgaaggctct gttcgggccg cgggcgtccg ttcctccgct ctgccggggt ctgggcggtg
     5761 tggagggtgg atctcagcgc tctctggaga gaagtggagc ggtgcgcaag actctgagcg
     5821 cagggatggc ggccctcagg agacactcgc actgcatctc catcaagctg caggcggacg
     5881 ctctgctgag tgtgatccgg cgtgcgaggc ctgtgttcct gcagtgtttg agtgtgaaga
     5941 cagatggagg aggctttgat gttcctgcac tgcggataca gctgaactcc acacacctgc
     6001 tgcccacact gcagctgtac aggacaggtt acccggagca catgtccctc agtgatttcc
     6061 gctgccgttt tcaggccctc tctgctcccg taatgaagcg atacggttct gtcttcatta
     6121 cccccgacga gaggaaggct gtggaggagc tactgatcga cctagatctg gacaagaaga
     6181 gtgtggtttt aggagccagc agagtgttta tgaagcgtgg cgtcctcagg tctttagatc
     6241 agcagaggga tcagttgttg agtggttggc tggtgcagct tcaagcctcg tgtctgggtc
     6301 acctgggccg cctcaaatac cgccgcatga aggttcagca gatggcagtg aagtgtgttc
     6361 agagaaatgt gcgggtgctc agtgcggtgt cccgctggag ctggtggaag ctgctgtgca
     6421 gagttcgccc cctgctggac gtcaacatgg acgaccagag gctccgatct aaagaggatg
     6481 agatcacagc tctgcgccgt cgtctggaga agtcggagaa ggagcgaaac gagttgcgtc
     6541 agactgcaga caacctggag accaaggtaa cagctgttat atcagagtta agtgatgagc
     6601 ggttcagagg agaggccatg agtcaagctt tagacgcaga gcgcactgag agactgagac
     6661 tcgccaaaga gagcaaggag ttacagagtc gtgtggagca gagtaaagtg tctttggagg
     6721 ccctggagag gcagttagag gaggaaaagc agaaagtgaa gaataaagaa gctctctgcg
     6781 gagtggccac agagagtgaa ctgcagctgc agctggagtg tgcgcagacg gaggtggagt
     6841 ttctgcgcag gcgtttgcgg cagacggagg aaagactgga gggagagaaa gaaggccggc
     6901 agctgctgga gacgaaggtc caggatctgc agtctcagct ggatcagtcc aaacgcacag
     6961 tgacagagct gaagcgccat tgcagacggc tgacctctga cctacaggat gcccgcgtgc
     7021 tcactgacag cctgcagagc cgtgctcacg agctggacag gaagcagagg aggtttgaca
     7081 gtgaactgac ccaggctctg acacatgctg ataatgagcg tgagcagaag gagagagtga
     7141 tccatgagaa caccacgctc ggcgctgaaa tattcaccct tcgcaagaca ctcaaggaga
     7201 gtgagacgga ggtccagcac ctgcagcagc tgaaggaaga gctgtcctgt cagatccgtg
     7261 acctcagtgt cccactcaaa ctcacctccg actccgttcc cgaactgaag aagcatctgc
     7321 gagagctgga gagcagagac aaggagcgct cggaggagat cagccagatg acggccagaa
     7381 tcacacagca tgagcagatg cacctgcgct tcgagatgga gatggagagg atgaagcaga
     7441 ttcatcagaa ggagctggag gacaaagatg aggagctgga ggacgtgcag aagtcctcac
     7501 agagacggct ccgacagctg gagatgcaac tggagcagga atatgaggag aaacagatgg
     7561 tggttcatga gaagcatgac ttagaggggc tgattgccac actgtgtgaa caggtgggcc
     7621 accgggactt tgacgtggag aagcgtctga ggcgagacct gaagcgaacc catgccctgc
     7681 tcagtgacgc tcagctgctc ctgtccactg ttgaacagcc tggtcagggt catgaccccg
     7741 gagcccagga gcagctgcag agactgcgct gccagctgga ggacagtgaa gcgcggcgtc
     7801 tggaggctga aggtgttcag aaaactctgg ctgcggagct ggaaaacgct cagatcgagc
     7861 tggagaacat ctgcaagcag aagagcctgg cggatgaaga tctgtctcag ctgcgctacg
     7921 agcggacaga cctgatgaag agactggagg aggaccagga ggacctgaat gagctcatga
     7981 agaagcacaa agccctcatc gctcagtctt ccagtgacat ctctcagatc cgagagcttc
     8041 aggctgagat ggaggagctg aagaagcaga gacagactct acagaaggag ctgcagacga
     8101 gtgtgtcgcg tgttcagttt ctggagtcgt ccaccgtgtc ccgcagcatc gtgagcaagc
     8161 aggaggcccg tatctgtgat ctggagaaca agctggagtt ccagagagga caggtcaaga
     8221 gatttgaggt gctggttctg cgtctgcggg acagtgtggt tcgtctgggt gaggagctgg
     8281 agcaggcagc acaggctgag gccagagaga gggagaactg cagatattac cagcagaggc
     8341 tggaggacat gaggatggag atggaggatc tgactggcag agagcaggac agcagcagga
     8401 gacgcatgga gctggagatg caggtggagg agctcagcgc cgtcagacag actctacagg
     8461 ccgatctgga gacgtccatc aggcggattg tggatctgca ggcggcgctg gaggaggttg
     8521 agtccagtga tgatagcgat acagaaagtg tgcagacggc ggtggagtcg ttctccagaa
     8581 agagagatct cgactccctg tccagcgtgg gctccagtgt gggcactgaa gacgttggtg
     8641 aagggatccg tcattgggcc ggtgtgccac gaggacctgg gagcagctcc ggacggcagt
     8701 ctgtcaccga cactatgagc acctacagtt tcagatcttg cactcaggac ctggatgaag
     8761 atgtgaaaga aagttctgga ggtctcggga gagcgtcttc atccacggct ctctccgagc
     8821 tcctggaggg tctgaggaag aagcgcgtcg gctgggaaca aggctcagat gtgggtgaag
     8881 gcagcaccgt ttctctgccc atctaccaga ccacaggagc ttcagctcta cgccgcagag
     8941 cctccgcact ctctctagaa gctgaagaag gtttagaaga gctgcctcga cctggaatac
     9001 tcaaacctcc cagtccacat ctcccccgcg cctccagcct ccgctcgctt ccagaagaaa
     9061 aaccagcagt gagtccagcc actgctactg tgatggggaa gatgaaccga ttcagctcct
     9121 gcgactctct gacgtccaac acaagctccc gacggcagat ctccaccctc gctatcccag
     9181 aagaagagga ggagagtaac acaaaaccct cgcctactat tgggtatcag cctgtgcgcc
     9241 ggcgtctgct aggcggattg gttgccgaat ccggagagtc acatttagga gcagaaccgc
     9301 tagttttcca gaaccgcaga ctcctggcca gcactcagga gagagcagac gtaggagact
     9361 ccagctcaga tattctgccg gccatcagac gagctcaatc caccagcagt ctggcgtcca
     9421 gcagcagccg ttcgggacag agacgagccc tcagcgtgca cttcggggag ctgccggtgt
     9481 ctagagcgtc ccgcagagag tccgactctg actcctccag ctcggggggg tctcagaagc
     9541 gccgcgggcc gcagggggag agactggagg ctgagggcag cgaggacgtc aactcggtca
     9601 tgaagaaata tcttaaaaaa gcagaagtgg acggatcagg ttcgggaatg gtgagcaagg
     9661 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     9721 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     9781 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     9841 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     9901 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     9961 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
    10021 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
    10081 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
    10141 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gttcgccgac cactaccagc
    10201 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
    10261 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
    10321 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt ggaagatctg
    10381 ggaattcaag gcctctcgag cctctagatt ctgcagccct atagcaccca gctttcttgt
    10441 acaaagtggg ggatccagac atgataagat acattgatga gtttggacaa accacaacta
    10501 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
    10561 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
    10621 ttcaggggga ggtgtgggag gttttttcca actttattat acatagttga taattcactg
    10681 gccgtcgttt tacggtacca tcgatgatga tccagacatg ataagataca ttgatgagtt
    10741 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
    10801 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
    10861 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
    10921 ctacaaatgt ggtatggctg attatgatcc tctagatcag atctgcgaag atacggccac
    10981 gggtgctctt gatcctgtgg ctgattttgg actgtgctgc tcgcagctgc tgatgaatca
    11041 catacttcct ccattttctt ccactgattg actgttataa tttccctaat ttccaggtca
    11101 aggtgctgtg cattgtggta atagatgtga catgacgtca cttccaaagg accaatgaac
    11161 atgtctgacc aatttcatat aatgtgaaaa cgattttcat aggcagaata aataacattt
    11221 aaattaaact gggcatcagc gcaattcaat tggtttggta atagcaaggg aaaatagaat
    11281 gaagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa
    11341 gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat ttttaattgt
    11401 actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca agtaaaatta
    11461 ctcaagtact ttacacctct ggttcttgac cccctacctt cagcaagccc agcagatcca
    11521 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
    11581 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    11641 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    11701 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    11761 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    11821 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    11881 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    11941 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    12001 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    12061 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    12121 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    12181 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    12241 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    12301 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    12361 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    12421 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    12481 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    12541 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    12601 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggc
    12661 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    12721 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    12781 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    12841 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    12901 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    12961 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    13021 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    13081 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    13141 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    13201 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    13261 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    13321 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    13381 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    13441 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    13501 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    13561 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    13621 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    13681 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    13741 ccccgaaaag tg
//
