LOCUS       sequence-339982-        3184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 15 kD Penn State ladder protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Santilli RT, Williamson JE 3rd, Shibata Y, Sowers RP, Fleischman AN,
            Tan S
  TITLE     The Penn State Protein Ladder system for inexpensive protein 
            molecular weight markers.
  JOURNAL   Sci Rep. 2021 Aug 18;11(1):16703. doi: 10.1038/s41598-021-96051-x.
  PUBMED    34408191
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-08-18"; volume: "11(1):16703. doi"; pages: " 
            10.1038/s41598-021-96051-x"
COMMENT     Sequence Label: pPSU15
FEATURES             Location/Qualifiers
     source          1..3184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             140..169
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(616..634)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      630..677
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        742..833
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1698..1737
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1768..2356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2257..2276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2510..2527
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2542..2681
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2607..2626)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2767..2789
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2783..2974)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggagagc tccaattgga attcaataat tttgtttaac tttaagaagg
      121 agatatacat atgggtagtc atcatcacca ccatcaccat catcatcatg gtagcgcaga
      181 taacaagttc aataaagaac agcagaacgc cttctatgaa attctgcatc tgccgaatct
      241 gaatgaagaa cagcgtaatg gttttatcca gagcctgaaa gatgatccga gccagagcgc
      301 aaatctgctg gccgaagcaa aaaaactgaa tgatgcacag gcaccgaaag gatccgctga
      361 taacaaattt aacaaagagc aacaaaacgc cttttacgag atcctgcatt taccgaacct
      421 gaccgaggaa cagcgcaacg gctttattca gtcactgaaa gacgacccga gtgtgagcaa
      481 agaaattctg gcagaggcca aaaagctgaa cgatgcccaa gctccggccg gctaatgtac
      541 aaagcttggt accacgcgtg cgcgctgatc cggctgctaa caaagcccga aaggaagctg
      601 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      661 tcttgagggg ttttttgctg aaaggaggaa ctatatcggg attagcccgc ctaatgagcg
      721 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      781 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      841 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      901 ctcacccaga aacgctcgtg aaagtaaaag acgcagagga ccaattgggg gcacgagtgg
      961 gatacataga actggacttg aatagcggta aaatccttga gagttttcgc cctgaagagc
     1021 gttttccaat gatgagcact ttcaaagttc tgctatgtgg agcagtatta tcccgtgtag
     1081 atgcggggca agagcaactc ggacgacgaa tacactattc gcagaatgac ttggttgaat
     1141 actccccagt gacagaaaag caccttacgg acggaatgac ggtaagagaa ttatgtagtg
     1201 ccgccataac gatgagtgat aacactgcgg cgaacttact tctgacaacc atcggtggac
     1261 cgaaggaatt aaccgctttt ttgcacaata tgggagacca tgtaactcgc cttgaccgtt
     1321 gggaaccaga actgaatgaa gccataccaa acgacgagcg agacaccaca atgcctgcgg
     1381 caatggcaac aacattacgc aaactattaa ctggcgaact acttactctg gcttcacggc
     1441 aacaattaat agactggctt gaagcggata aagttgcagg accactactg cgttcggcac
     1501 ttcctgctgg ctggtttatt gctgataaat ctggggcagg agagcgtggt tcacggggta
     1561 tcattgccgc acttggacca gatggtaagc cttcccgtat cgtagttatc tacacgacgg
     1621 gtagtcaggc aactatggac gaacgaaata gacagattgc tgaaataggg gcttcactga
     1681 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacgg
     1741 accggtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1801 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1861 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1921 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1981 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2041 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2101 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2161 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2221 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2281 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2341 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2401 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2461 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2521 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2581 ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2641 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     2701 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     2761 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     2821 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     2881 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     2941 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
     3001 tgttcatggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     3061 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     3121 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     3181 tgtt
//
