LOCUS       sequence-339989-       12133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes OptoQ-AMP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12133)
  AUTHORS   Lalwani MA, Zhao EM, Wegner SA, Avalos JL
  TITLE     The Neurospora crassa Inducible Q System Enables Simultaneous 
            Optogenetic Amplification and Inversion in Saccharomyces cerevisiae 
            for Bidirectional Control of Gene Expression.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):2060-2075. doi: 
            10.1021/acssynbio.1c00229. Epub 2021 Aug 4.
  PUBMED    34346207
REFERENCE   2  (bases 1 to 12133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00229"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "2060-2075"
COMMENT     Sequence Label: pMAL669
FEATURES             Location/Qualifiers
     source          1..12133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(13..31)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(52..68)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(52..68)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(65..87)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    76..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(100..106,107..124,125..130))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    145..166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(283..300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(454..1042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(534..553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1213..2004,2005..2073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1836..1855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2074..2178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2262..2280
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2318..2340)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2440..2459
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2815..3270)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2952..2973)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3164..3183
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3396..3418
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3410..3427
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3411..3427
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3434..3453
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3434..3452
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4116..4513
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             4543..4563
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5452..5699
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5468..5486)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        5870..5901
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             7668..8375
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(7692..7720)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8315..8335
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      8391..8556
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8446..8470)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        complement(10020..10051)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     promoter        complement(11633..11820)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 cagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct
       61 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      121 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
      301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2161 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcc tcggcgcgtg
     2221 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2281 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     2341 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2401 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     2461 tgagagtgca ccataccacc ttttcaattc atcatttttt ttttattctt ttttttgatt
     2521 tcggtttcct tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa
     2581 ggagcacaga cttagattgg tatatatacg catattgggt cagcagtaca gaaccctcga
     2641 cgaagttgta agtggtggat cagatatcat cattgttggc agaggacttt tcgccaaggg
     2701 tagagatcct aatactaaac tcacaaatta gagcttcaat ttaattatat cagttattac
     2761 cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg
     2821 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     2881 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2941 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     3001 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     3061 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     3121 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     3181 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     3241 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg
     3301 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     3361 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     3421 ggccagtgaa ttgtaatacg actcactata gggggccagc gtggccgttt aaacatttaa
     3481 atggccggcc gctgccaccg ttgtttccac cgaagaaacc accgttgccg taaccaccac
     3541 gacggttgtt gctaaagaag ctgccaccgc cacggccacc gttgtagccg ccgttgttgt
     3601 tattgtagtt gctactgtta tttctggcac ttcttggttt tcctcttaag tgaggaggaa
     3661 cataaccatt ctcgttgttg tcgttgatgc ttaaattttg cacttgttcg ctcagttcag
     3721 ccataatatg aaatgctttt cttgttgttc ttacggaata ccacttgcca cctatcacca
     3781 caactaactt tttcccgttc ctccatctct tttatatttt ttttctggag ttcaagagaa
     3841 aaaaaaagaa aaagcaaaaa gaaaaaagga aagcgcgcct cgttcagaat gacacgtata
     3901 gaatgatgca ttaccttgtc atcttcagta tcatactgtt cgtatacata cttactgaca
     3961 ttcataggta tacatatata cacatgtata tatatcgtat gctgcagctt taaataatcg
     4021 gtgtcaagat ctgagctcca ccgcggtggc ggccgcttat ttactcagga tcccgcatgt
     4081 tgcaggtggg agttccagct cccgggaaac agctgatagc ttcaaaatgt ttctactcct
     4141 tttttactct tccagatttt ctcggactcc gcgcatcgcc gtaccacttc aaaacaccca
     4201 agcacagcat actaaatttc ccctctttct tcctctaggg tgtcgttaat tacccgtact
     4261 aaaggtttgg aaaagaaaaa agagaccgcc tcgtttcttt ttcttcgtcg aaaaaggcaa
     4321 taaaaatttt tatcacgttt ctttttcttg aaaatttttt ttttgatttt tttctctttc
     4381 gatgacctcc cattgatatt taagttaata aacggtcttc aatttctcaa gtttcagttt
     4441 catttttctt gttctattac aacttttttt acttcttgct cattagaaag aaagcatagc
     4501 aatctaatct aagttttggc ggccgctaaa atcatggcta gcccgaaaaa aaagagaaaa
     4561 gtagccccac caacggacgt ttcacttggt gacgaattac atttagacgg tgaggatgta
     4621 gctatggcac atgccgatgc tcttgatgat tttgatctgg acatgttagg tgatggggat
     4681 tctcccggcc ccggatttac accacatgat tctgctccgt atggcgcgtt ggacatggca
     4741 gatttcgaat ttgaacagat gtttactgat gcacttggta tcgacgaata tggaggtgaa
     4801 ttcggagcag atgatactag agttgaagtc caacctccag cccaatgggt attggatttg
     4861 atcgaggcct cacccatagc aagtgttgtt agtgatccca gattggctga taatcctttg
     4921 attgctatca accaggcctt taccgatcta actgggtatt ctgaggaaga atgtgttggt
     4981 cgtaattgta gatttttggc aggatctggt actgaaccct ggctaacaga taagatccgt
     5041 caaggtgttc gtgagcataa acccgtgttg gttgagattt tgaattacaa aaaggatggt
     5101 actccttttc gtaatgcagt tttggtggca ccaatctatg atgacgatga tgaattacta
     5161 tacttccttg gtagccaagt ggaagtggat gacgatcaac cgaacatggg catggcccgt
     5221 agagaacgtg ctgcggagat gttaaaaact ctttcaccac gtcaattaga agtcacaact
     5281 ctggttgcat caggcctaag aaataaagaa gtggctgcca gactgggtct ttcagaaaaa
     5341 actgtcaaaa tgcacagagg tttggtaatg gaaaaattaa acttaaaaac gagtgcagat
     5401 ttagttagaa ttgccgtaga agcaggtatt taagtcgacc tcgagtaatg atcatgtaat
     5461 tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag
     5521 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa
     5581 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta
     5641 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg
     5701 gccttatcgc cgggtacgtg agttcgccag cttcgagtag gtagccttta gtccatgcgt
     5761 tataggtagc ctttagtcca tgcgttatag gtagccttta gtccatgcgt tataggtagc
     5821 ctttagtcca tgcgttatag gtagccttta gtccatgctt aagagacact agagggtata
     5881 taatggaagc tcgacttcca gcttggcaat ccggtactgt tggtaaagcc accgcggccg
     5941 ctaaaatcat gccacccaag agaaagacgc tgaacgcggc agcggaagct aacgcccatg
     6001 cagatggcca cgcggacggt aatgcggacg gtcatgtagc taacaccgcg gcttcctcta
     6061 acaacgcacg tttcgctgac ttgacgaata tcgatacgcc agggttggga ccgactacta
     6121 ccaccttatt ggtggagcct gccaggtcta aaagacaaag ggtcagtcgt gcgtgtgacc
     6181 aatgcagagc cgctcgtgaa aaatgtgacg gtattcagcc tgcatgtttt ccttgcgtga
     6241 gtcagggcag gtcttgcacg tatcaggctt cccccaagaa gcgtggcgtt cagactggtt
     6301 atattaggac cttggaattg gcgttggcgt ggatgtttga aaacgtagca aggtccgaag
     6361 acgcgctaca taatctatta gtcagggacg ctggccaagg ctcagcgtta ctggtaggga
     6421 aggattcacc ggcggcggaa agactgcatg caaggtgggc gacctccagg gtcaataaaa
     6481 gtatcacaag gcttctccgt caattagagc tgccgcccac ggctactgcc actgcgtcaa
     6541 tcatgcctca cgtcatggaa caacccttga gtacgtcaat aaaccctgtt aatgatcgtt
     6601 ttaatgggat tccaaatccg acgccttaca attctgacgc tgctcttgac gctataaccc
     6661 aaaccaacga ttacggttcc gtgaataccc acggaatcct gagcacctac ccccctccag
     6721 caacacacct taacgaggcg tcagtagctc tagctccggg aggggcgcct ccgcgtccac
     6781 ccccaccgta cgtggactca acgactaatc accctcccta tcatagcaac ctggtcccta
     6841 tggcaaattt tggttacagt acagttgatt atgatgccat ggtagatgat ttagcgagta
     6901 ttgaatatac tgatgctgta gatgttgacc cacaatttat gacgaatctt gggttcgtcc
     6961 ctgggtgcaa tttcagtgac attaacacgt atgaacaata atgactcgag taatgatcat
     7021 ctctgctttt gtgcgcgtat gtttatgtat gtacctctct ctctatttct atttttaaac
     7081 caccctctca ataaaataaa aataataaag tatttttaag gaaaagacgt gtttaagcac
     7141 tgactttatc tactttttgt acgttttcat tgatataatg tgttttgtct ctcccttttc
     7201 tacgaaaatt tcaaaaattg accaaaaaaa ggaatatata tacgaaaaac tattatattt
     7261 atatatcata gtgttgataa aaaatgttta tccattggac cgtgtatcat atttatcgcc
     7321 gggttatcgc cgggccagct taagaataaa ttaaactagt gggtaatcgc ttatcctagg
     7381 ataaacaatt atcctcaagg gtaatcgctt atcctctagg gtaatcgctt atcctagggt
     7441 aatcgcttat cctttctaga tatatatatg tgtgcgacga cacatgatca tatggcatgc
     7501 atgtgctctg tatgtatata aaactcttgt tttcttcttt tctctaaata ttctttcctt
     7561 atacattagg acctttgcag cataaattac tatacttcta tagacacaca aacacaaata
     7621 cacacactaa attaatagcg gccgctaaaa tcatggctag cgtcgacaaa ggagaagaac
     7681 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     7741 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     7801 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcacttatg
     7861 gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg
     7921 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     7981 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     8041 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     8101 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     8161 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     8221 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     8281 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     8341 ctgggattac acatggcatg gatgaactat acaaactcga gtaatgatca gcgaatttct
     8401 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     8461 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     8521 tcaggttgct ttctcaggta tagcatgagg tcgctcttat cgccggcgaa taaattaagg
     8581 cgcgccttaa tttattcgcc ggcgataaat atgatacacg gtccaatgga taaacatttt
     8641 ttatcaacac tatgatatat aaatataata gtttttcgta tatatattcc tttttttggt
     8701 caatttttga aattttcgta gaaaagggag agacaaaaca cattatatca atgaaaacgt
     8761 acaaaaagta gataaagtca gtgcttaaac acgtcttttc cttaaaaata ctttattatt
     8821 tttattttat tgagagggtg gtttaaaaat agaaatagag agagaggtac atacataaac
     8881 atacgcgcac aaaagcagag atgatcatta ctcgagtcat tattgttcat acgtgttaat
     8941 gtcactgaaa ttgcacccag ggacgaaccc aagattcgtc ataaattgtg ggtcaacatc
     9001 tacagcatca gtatattcaa tactcgctaa atcatctacc atggcatcat aatcaactgt
     9061 actgtaacca aaatttgcca tagggaccag gttgctatga tagggagggt gattagtcgt
     9121 tgagtccacg tacggtgggg gtggacgcgg aggcgcccct cccggagcta gagctactga
     9181 cgcctcgtta aggtgtgttg ctggaggggg gtaggtgctc aggattccgt gggtattcac
     9241 ggaaccgtaa tcgttggttt gggttatagc gtcaagagca gcgtcagaat tgtaaggcgt
     9301 cggatttgga atcccattaa aacgatcatt aacagggttt attgacgtac tcaagggttg
     9361 ttccatgacg tgaggcatga ttgacgcagt ggcagtagcc gtgggcggca gctctaattg
     9421 acggagaagc cttgtgatac ttttattgac cctggaggtc gcccaccttg catgcagtct
     9481 ttccgccgcc ggtgaatcct tccctaccag taacgctgag ccttggccag cgtccctgac
     9541 taatagatta tgtagcgcgt cttcggacct tgctacgttt tcaaacatcc acgccaacgc
     9601 caattccaag gtcctaatat aaccagtctg aacgccacgc ttcttggggg aagcctgata
     9661 cgtgcaagac ctgccctgac tcacgcaagg aaaacatgca ggctgaatac cgtcacattt
     9721 ttcacgagcg gctctgcatt ggtcacacgc acgactgacc ctttgtcttt tagacctggc
     9781 aggctccacc aataaggtgg tagtagtcgg tcccaaccct ggcgtatcga tattcgtcaa
     9841 gtcagcgaaa cgtgcgttgt tagaggaagc cgcggtgtta gctacatgac cgtccgcatt
     9901 accgtccgcg tggccatctg catgggcgtt agcttccgct gccgcgttca gcgtctttct
     9961 cttgggtggc atgattttag cggccgcggt ggctttacca acagtaccgg attgccaagc
    10021 tggaagtcga gcttccatta tataccctct agtgtctctt aagcatggac taaaggctac
    10081 ctataacgca tggactaaag gctacctata acgcatggac taaaggctac ctataacgca
    10141 tggactaaag gctacctata acgcatggac taaaggctac ctactcgaag ctggcgaact
    10201 cacgtacccg gaggtaccca aagccgaatc caccacggtc accgttgttt ctgaagaaac
    10261 caccacggcc gccaccgttg ttgaaaccac cacgaggagc agaatcgcct cttctgccac
    10321 caccgttacg gatgtgaggt ggaacgtaac caccacgtct ttgttgccct tcaggcatgg
    10381 aaacttgctc tgctaactca gtcatattgg atatatgtat tactttcttt attttttgta
    10441 gtttagtctt ctatttcttt tatagtttgg tatctttttg caaaaaattt acctctatat
    10501 cgtgttcgtg cgtatttctt cctctataat atgaaaagaa aggcagtagt cttggttttc
    10561 tattattcca aatattacaa gaatacccag aaccacaaga agcagaagaa actaactgct
    10621 ttatatactt aatttcccgc cacaagctca tcgcccagaa gtttttttca gtttttcacc
    10681 tgacaatctg gcagctcgct gaaaaatttt cacctggggt cgcctgctac gtagagtgat
    10741 cacgtttatg aatagcacat tatatagtgg cccagacggt ctgatggtag ttcatgtagt
    10801 taagaataaa tactctgttt gtgtgatttc tatgtgtacg ttatctatat aaatattgtt
    10861 gatggtggta gctgtgggct gtgttctatg ctaggacacc cttagtggat ggaacgtcat
    10921 cgcgacccgt aggtgttctt gcctctctga tggcgatggc gagcgccttg aaagctgatt
    10981 cggagcggtg gtggtcgttg gtgccccgca gacagtccac atgcatggtg atacgcgcct
    11041 cagtggcgaa actctccaag aagtgcggga tcatctctgt ggatagctgg cctatgcgtt
    11101 ctcgcttcag gcccagctcc accacggcga aaggcctatt ggagaagtca accacagcgc
    11161 ggctcagcgc ctcgtccagt ggtgcaaacc catgcccgaa cctcttgata ccacgcacgg
    11221 agccaagcgc ctccttgaac gcttggccca gcgcaatccc acagtcctca acagtgtggt
    11281 ggtcatcgat gtgcaagtcc cctatacact ccaggatcag agaccacccg ccatgcttcg
    11341 ccagcgcagt caacatgtgg tccaaaaacc caactccagt gtgcacatgg atggtctgcc
    11401 catcaccact agcatattcc ttgcccagta tgctctcccg tacagaaaca gacccaccgt
    11461 caagatccag cgccagctgt atattcgtct cctgcgtaac cctcttaaca aacgccatcc
    11521 tttgctcgat gcttctcttt gcttcaatta tctcaacttt aagtaaacct gaagtaaact
    11581 agaactagat taaatcagaa ggcatccgca gctttatata catatagagc ctctttgcct
    11641 tcgtttatct tgcctgctca ttttttagta tattcttcga agaaatcaca ttactttata
    11701 taatgtataa ttcattatgt gataatgcca atcgctaaga aaaaaaaaga gtcatccgct
    11761 aggtggaaaa aaaaaaatga aaatcattac cgaggcataa aaaaatatag agtgtactag
    11821 aggaggccaa gagtaataga aaaagaaaat tgcgggaaag gactgtgtta tgacttccct
    11881 gactaatgcc gtgttcaaac gatacctggc agtgactcct agcgctcacc aagctcttaa
    11941 aacgagaatt aagaaaaagt cgtcatcttt cgataagttt ttcccacagc aaagcaatag
    12001 tagaaaaaaa caatgggaaa cgttgaatga agacaaagcg tcgtggttta aaaggaaata
    12061 cgctcacgta catgctaggg aacaggaccg tgcagcggcc agcgtggccg tttaaacatt
    12121 taaatggccg gcc
//
