LOCUS       sequence-339992-        3082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 10 kD Penn State ladder protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Santilli RT, Williamson JE 3rd, Shibata Y, Sowers RP, Fleischman AN,
            Tan S
  TITLE     The Penn State Protein Ladder system for inexpensive protein 
            molecular weight markers.
  JOURNAL   Sci Rep. 2021 Aug 18;11(1):16703. doi: 10.1038/s41598-021-96051-x.
  PUBMED    34408191
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-08-18"; volume: "11(1):16703. doi"; pages: " 
            10.1038/s41598-021-96051-x"
COMMENT     Sequence Label: pPSU10
FEATURES             Location/Qualifiers
     source          1..3082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..175
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             185..214
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(514..532)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      528..575
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        640..731
                     /gene="bla"
                     /label=AmpR promoter
     terminator      1596..1635
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1666..2254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2155..2174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2408..2425
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2440..2579
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2505..2524)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2665..2687
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2681..2872)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggagagc tccaattgga attcgctagc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatggcaag ctggtcacat ccgcagtttg aaaaaggtag
      181 cagtcatcat caccaccatc accatcatca tcacagcagc ggtagcggta gcgcagataa
      241 caaatttaac aaagaacagc agaacgcctt ctacgaaatt ctgcatctgc cgaatctgaa
      301 tgaagaacag cgtaatggtt ttatccagag cctgaaagat gatccgagcc agagcgcaaa
      361 tctgctggcc gaagcaaaaa aactgaatga tgcacaggca ccgaaaggat cctaagccgg
      421 ctgtacaaga tccagatcta agcttggtac cacgcgtgcg cgctgatccg gctgctaaca
      481 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      541 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatcgggat
      601 tagcccgcct aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac
      661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      721 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      781 tttgccttcc tgtttttgct cacccagaaa cgctcgtgaa agtaaaagac gcagaggacc
      841 aattgggggc acgagtggga tacatagaac tggacttgaa tagcggtaaa atccttgaga
      901 gttttcgccc tgaagagcgt tttccaatga tgagcacttt caaagttctg ctatgtggag
      961 cagtattatc ccgtgtagat gcggggcaag agcaactcgg acgacgaata cactattcgc
     1021 agaatgactt ggttgaatac tccccagtga cagaaaagca ccttacggac ggaatgacgg
     1081 taagagaatt atgtagtgcc gccataacga tgagtgataa cactgcggcg aacttacttc
     1141 tgacaaccat cggtggaccg aaggaattaa ccgctttttt gcacaatatg ggagaccatg
     1201 taactcgcct tgaccgttgg gaaccagaac tgaatgaagc cataccaaac gacgagcgag
     1261 acaccacaat gcctgcggca atggcaacaa cattacgcaa actattaact ggcgaactac
     1321 ttactctggc ttcacggcaa caattaatag actggcttga agcggataaa gttgcaggac
     1381 cactactgcg ttcggcactt cctgctggct ggtttattgc tgataaatct ggggcaggag
     1441 agcgtggttc acggggtatc attgccgcac ttggaccaga tggtaagcct tcccgtatcg
     1501 tagttatcta cacgacgggt agtcaggcaa ctatggacga acgaaataga cagattgctg
     1561 aaataggggc ttcactgatt aagcattggt aaaccgatac aattaaaggc tccttttgga
     1621 gccttttttt ttggacggac cggtagaaaa gatcaaagga tcttcttgag atcctttttt
     1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1801 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2341 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2401 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     2461 tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat
     2521 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     2581 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     2641 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     2701 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
     2761 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     2821 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     2881 ccgtgtaagg gggatttctg ttcatggggt aatgataccg atgaaacgag agaggatgct
     2941 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     3001 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     3061 cgttaataca gatgtaggtg tt
//
