LOCUS       sequence-339995-        3835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 40 kD Penn State ladder protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Santilli RT, Williamson JE 3rd, Shibata Y, Sowers RP, Fleischman AN,
            Tan S
  TITLE     The Penn State Protein Ladder system for inexpensive protein 
            molecular weight markers.
  JOURNAL   Sci Rep. 2021 Aug 18;11(1):16703. doi: 10.1038/s41598-021-96051-x.
  PUBMED    34408191
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-08-18"; volume: "11(1):16703. doi"; pages: " 
            10.1038/s41598-021-96051-x"
COMMENT     Sequence Label: pPSU40
FEATURES             Location/Qualifiers
     source          1..3835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             140..169
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             533..1186
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKF
                     ELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYG
                     VSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLY
                     MDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1141..1163
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(1267..1285)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1281..1328
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1393..1484
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2349..2388
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      2419..3007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2908..2927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3161..3178
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3193..3332
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3258..3277)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3418..3440
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3434..3625)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggagagc tccaattgga attcaataat tttgtttaac tttaagaagg
      121 agatatacat atgggtagtc atcatcacca ccatcaccat catcatcatg gtagcgcaga
      181 taacaagttc aataaagaac agcagaacgc cttctatgaa attctgcatc tgccgaatct
      241 gaatgaagaa cagcgtaatg gttttatcca gagcctgaaa gatgatccga gccagagcgc
      301 aaatctgctg gccgaagcaa aaaaactgaa tgatgcacag gcaccgaaag gatccgctga
      361 taacaaattt aacaaagagc aacaaaacgc cttttacgag atcctgcatt taccgaacct
      421 gaccgaggaa cagcgcaacg gctttattca gtcactgaaa gacgacccga gtgtgagcaa
      481 agaaattctg gcagaggcca aaaagctgaa cgatgcccaa gctccggccg gctcccctat
      541 actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct
      601 tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa
      661 aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa
      721 attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg
      781 ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata
      841 cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag
      901 caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa
      961 tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata
     1021 catggaccca atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga
     1081 agctatccca caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca
     1141 gggctggcaa gccacgtttg gtggtggcga ccatcctcca aaataatgta caaagcttgg
     1201 taccacgcgt gcgcgctgat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     1261 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     1321 gttttttgct gaaaggagga actatatcgg gattagcccg cctaatgagc gggctttttt
     1381 ttaattcccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1441 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1501 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1561 aaacgctcgt gaaagtaaaa gacgcagagg accaattggg ggcacgagtg ggatacatag
     1621 aactggactt gaatagcggt aaaatccttg agagttttcg ccctgaagag cgttttccaa
     1681 tgatgagcac tttcaaagtt ctgctatgtg gagcagtatt atcccgtgta gatgcggggc
     1741 aagagcaact cggacgacga atacactatt cgcagaatga cttggttgaa tactccccag
     1801 tgacagaaaa gcaccttacg gacggaatga cggtaagaga attatgtagt gccgccataa
     1861 cgatgagtga taacactgcg gcgaacttac ttctgacaac catcggtgga ccgaaggaat
     1921 taaccgcttt tttgcacaat atgggagacc atgtaactcg ccttgaccgt tgggaaccag
     1981 aactgaatga agccatacca aacgacgagc gagacaccac aatgcctgcg gcaatggcaa
     2041 caacattacg caaactatta actggcgaac tacttactct ggcttcacgg caacaattaa
     2101 tagactggct tgaagcggat aaagttgcag gaccactact gcgttcggca cttcctgctg
     2161 gctggtttat tgctgataaa tctggggcag gagagcgtgg ttcacggggt atcattgccg
     2221 cacttggacc agatggtaag ccttcccgta tcgtagttat ctacacgacg ggtagtcagg
     2281 caactatgga cgaacgaaat agacagattg ctgaaatagg ggcttcactg attaagcatt
     2341 ggtaaaccga tacaattaaa ggctcctttt ggagcctttt tttttggacg gaccggtaga
     2401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2461 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2521 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     2581 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2641 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2701 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2761 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2821 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2881 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2941 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3001 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3061 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3121 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3181 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3241 caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     3301 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     3361 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     3421 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     3481 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     3541 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     3601 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     3661 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
     3721 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca
     3781 gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgtt
//
