LOCUS       sequence-340000-        5407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 100 kD Penn State ladder protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Santilli RT, Williamson JE 3rd, Shibata Y, Sowers RP, Fleischman AN,
            Tan S
  TITLE     The Penn State Protein Ladder system for inexpensive protein 
            molecular weight markers.
  JOURNAL   Sci Rep. 2021 Aug 18;11(1):16703. doi: 10.1038/s41598-021-96051-x.
  PUBMED    34408191
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-08-18"; volume: "11(1):16703. doi"; pages: " 
            10.1038/s41598-021-96051-x"
COMMENT     Sequence Label: pPSU100
FEATURES             Location/Qualifiers
     source          1..5407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..175
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             185..214
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(2839..2857)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2853..2900
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2965..3056
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3921..3960
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      3991..4579
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4480..4499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4733..4750
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4765..4904
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4830..4849)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4990..5012
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5006..5197)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt
       61 tccctccggc tccggagagc tccaattgga attcgctagc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatggcaag ctggtcacat ccgcagtttg aaaaaggtag
      181 cagtcatcat caccaccatc accatcatca tcacagcagc ggtagcggta gcgcagataa
      241 caaatttaac aaagaacagc agaacgcctt ctacgaaatt ctgcatctgc cgaatctgaa
      301 tgaagaacag cgtaatggtt ttatccagag cctgaaagat gatccgagcc agagcgcaaa
      361 tctgctggcc gaagcaaaaa aactgaatga tgcacaggca ccgaaaggat ctgctgataa
      421 caaatttaac aaagagcaac aaaacgcctt ttacgagatc ctgcatttac cgaacctgac
      481 cgaggaacag cgcaacggct ttattcagtc actgaaagac gacccgagtg tgagcaaaga
      541 aattctggca gaggccaaaa agctgaacga tgcccaagct ccgaaaggat ccggtagcag
      601 cggtagcagt ggttcaagcg caccggttcg tagcctgaat tgtaccctgc gtgatagcca
      661 gcagaaaagc ctggttatga gtggtccgta tgaactgaaa gcactgcatc tgcaaggtca
      721 ggatatggaa cagcaggttg tttttagcat gagctttgtt cagggtgaag aaagcaatga
      781 taaaattccg gttgcactgg gtctgaaaga gaaaaatctg tatctgagct gcgtgctgaa
      841 agatgataaa ccgaccttac agctggaaag cgttgatccg aaaaactacc cgaaaaagaa
      901 aatggaaaaa cgctttgtgt tcaacaaaat cgagatcaac aacaaacttg agtttgagag
      961 cgcacagttt ccgaattggt atattagcac cagccaggcc gaaaatatgc cggttttttt
     1021 aggtggcacc aaaggtggcc aggatattac cgattttacc atgcagtttg tgtccagcgc
     1081 cggcagtgag gcagaagccc gcccgactaa ctttatccgt cagatcatcg atgaagatct
     1141 ggccagtggt aagcacacca cagtacacac ccgtttcccg ccggagccga atggctatct
     1201 gcatattggc catgcgaaat ctatctgcct gaacttcggg atcgcccagg actataaagg
     1261 ccagtgcaac ctgcgtttcg acgacactaa cccggtaaaa gaagatatcg agtatgttga
     1321 gtcgatcaaa aacgacgtag agtggttagg ttttcactgg tctggtaacg tccgttactc
     1381 ctccgattat tttgatcagc tccacgccta tgcgatcgaa ctgatcaata aaggcctggc
     1441 gtacgttgat gaactgacgc cggaacagat ccgcgaatac cgcggcaccc tgacgcaacc
     1501 gggtaaaaac agcccgtacc gcgaccgcag cgttgaagag aacctggcgc tgttcgaaaa
     1561 aatgcgtgcc ggtggttttg aagaaggtaa agcctgcctg cgtgcgaaaa tcgacatggc
     1621 ttcaccgttt atcgtgatgc gcgatccggt gctgtaccgt attaaatttg ctgaacacca
     1681 ccagactggc aacaagtggt gcatctaccc gatgtacgac ttcacccact gcatcagcga
     1741 tgccctggaa ggtattacgc actctctgtg tacgcttgag ttccaggaca accgtcgtct
     1801 gtacgactgg gtactggaca acatcacgat tcctgttcac ccgcgccagt atgagttctc
     1861 gcgcctgaat ctggaataca ccgtgatgtc caagcgtaag ttgaacctgc tggtgaccga
     1921 caagcacgtt gaaggctggg atgacccgcg tatgccgacc atttccggtc tgcgtcgtcg
     1981 tggttacact gcggcttcta ttcgtgagtt ctgcaaacgc atcggcgtga ccaagcagga
     2041 caacaccatt gagatggcgt cgctggaatc ctgcatccgt gaagatctca acgaaaatgc
     2101 gccgcgcgca atggcggtta tcgatccggt gaaactggtt atcgaaaact atcagggcga
     2161 aggcgaaatg gttaccatgc cgaaccatcc gaacaaaccg gaaatgggca gccgtcaggt
     2221 gccgtttagc ggtgagattt ggattgatcg cgccgatttc cgcgaagaag ctaacaagca
     2281 gtacaaacgt ctggtgctgg gtaaagaagt gcgtctgcgt aatgcttatg tgattaaggc
     2341 agaacgtgtc gagaaagatg ccgaaggtaa tatcaccacc atcttctgta cttatgacgc
     2401 cgatacctta agcaaagatc cggcagatgg tcgtaaagtc aaaggtgtta ttcactgggt
     2461 gagcgcggca catgcgctgc cggttgaaat ccgtttgtat gaccgtctgt tcagcgtgcc
     2521 taacccaggt gctgcggatg atttcctgtc ggtgattaac ccggaatcgc tggtgatcaa
     2581 acagggcttt gctgaaccgt cgctgaaaga tgcggttgcg ggtaaagcat tccagtttga
     2641 gcgtgaaggt tacttctgcc tcgatagccg ccattctacg gcggaaaaac cggtatttaa
     2701 ccgcaccgtt gggctgcgtg atacctgggc gaaagtaggc gagtaatgta caagatccag
     2761 atctaagctt ggtaccacgc gtgcgcgctg atccggctgc taacaaagcc cgaaaggaag
     2821 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     2881 gggtcttgag gggttttttg ctgaaaggag gaactatatc gggattagcc cgcctaatga
     2941 gcgggctttt ttttaattcc cctatttgtt tatttttcta aatacattca aatatgtatc
     3001 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3061 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3121 ttgctcaccc agaaacgctc gtgaaagtaa aagacgcaga ggaccaattg ggggcacgag
     3181 tgggatacat agaactggac ttgaatagcg gtaaaatcct tgagagtttt cgccctgaag
     3241 agcgttttcc aatgatgagc actttcaaag ttctgctatg tggagcagta ttatcccgtg
     3301 tagatgcggg gcaagagcaa ctcggacgac gaatacacta ttcgcagaat gacttggttg
     3361 aatactcccc agtgacagaa aagcacctta cggacggaat gacggtaaga gaattatgta
     3421 gtgccgccat aacgatgagt gataacactg cggcgaactt acttctgaca accatcggtg
     3481 gaccgaagga attaaccgct tttttgcaca atatgggaga ccatgtaact cgccttgacc
     3541 gttgggaacc agaactgaat gaagccatac caaacgacga gcgagacacc acaatgcctg
     3601 cggcaatggc aacaacatta cgcaaactat taactggcga actacttact ctggcttcac
     3661 ggcaacaatt aatagactgg cttgaagcgg ataaagttgc aggaccacta ctgcgttcgg
     3721 cacttcctgc tggctggttt attgctgata aatctggggc aggagagcgt ggttcacggg
     3781 gtatcattgc cgcacttgga ccagatggta agccttcccg tatcgtagtt atctacacga
     3841 cgggtagtca ggcaactatg gacgaacgaa atagacagat tgctgaaata ggggcttcac
     3901 tgattaagca ttggtaaacc gatacaatta aaggctcctt ttggagcctt tttttttgga
     3961 cggaccggta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4021 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4081 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     4141 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4201 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4261 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4321 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4381 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4441 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4501 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4561 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4621 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4681 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4741 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     4801 tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4861 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     4921 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4981 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5041 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt
     5101 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
     5161 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
     5221 ttctgttcat ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
     5281 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
     5341 gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt
     5401 aggtgtt
//
