LOCUS       sequence-340009-        8813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms 
            d/f of DAF-16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH3377
FEATURES             Location/Qualifiers
     source          1..8813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5941..5958)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5941..5957)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5950..5972)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6170..6625
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6257..6276)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6467..6488
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6642..6661)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6761..6783
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6821..6839)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6907..7011
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7012..7080,7081..7872)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7230..7249)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8043..8631
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8532..8551
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8785..8802
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgaatg
      241 gccatgggac gtcgacgcat agcccgcagt cacttgaaag cacatttaag atggaatcaa
      301 atggtaagaa agctcgtgta atcaggagat tttatccggg gtatgccgag gaatatatgg
      361 tgcactcgca ttagctggca ttttttgtaa ttatcggatt tctacctttt tcttaatcta
      421 gtttgttatc attgtccatc atttacaacc aaaaatttgc gtcatttaca accaaaaatt
      481 gatctgacaa ttatcataat tcggttttta ttttcagaag aaacgctccg atgtgatcct
      541 atagtgaata tacttttcca tcctctctga aataaacaat tatgaagaat gcatcaattt
      601 gcattcgaaa taactgccgt attttgtcta ttggtatggc tgcagtacca tttttcttcg
      661 cgtctttgcg cagccttcgg gcgctaccaa taaggctaag ccggggtaag ttatttcaga
      721 attctaatca actaatctaa gtctcataca tcattgtcaa gtgacgcatt tttttaattt
      781 ctatgttaac tcgactttaa tattcttttc tggtttcacc agaaaaatgc tggactgaaa
      841 aattgaattt cccgcgattt gggaaaaacc gcagtttaag cttttggcat gaatacagtg
      901 aacacaggaa caaaaaattg acttgttatt ttgagtttat attaaatgtt aattttgcat
      961 ctcatatttt cacaaaactt gtttgcttga aaaatgtgca aataacaagt gaacatacat
     1021 caaggtttat gcagatcagc actgatgcaa ctgatagcaa aactgataag ggtcaaaatt
     1081 tcagtggcca gcacaaacac atacacattc gtagttcgtt ttgtgtttcg tgtatttagc
     1141 atttattttt caattccggc aatttgccga tttgccggaa attttgattt ttggcaaatt
     1201 gccagtttgc cgtttgccga acatcaattt gccgaaattt tttagaggga tctttataag
     1261 acggaaacac ttaaaattgt gcttttttgg aatttttttc aatgtttcaa tagaattagc
     1321 ttacatttaa aattagatgt aggaacgttt ataggacgct tacaattttg ccgattaaaa
     1381 tcgaagttct gaacttttca aaaaatgtgc aaaatttttt ttgacaaatt tgacaaatcg
     1441 gccggtttgt cgattgtccg gaatttttca gtttcggcga tttaccggtt tgccggaaaa
     1501 catcgtttgc cacccacaca cacataaaga gacgaaacaa cacaccatct tcaaaaaatt
     1561 tgtgaatctt catttccacg tggtttgcag ggtttcccat ccaccccact ctatctctcc
     1621 aagttgaatt ttggcaaaat tgaccacaca ccgatcaaaa actattgtgt gtgtgtgtat
     1681 ttgtgtcttc aataattcct gtctgtgtct cgcactatct attgactaca agaacgtgat
     1741 cgcagctcta cgaaaccttt actttatttt agaaatattt tttcatgcta ttagcaaaaa
     1801 aaaagttttt ttttgtctaa gtacacttaa aattttccaa aatttttaag atttttcaaa
     1861 atgtgcgatc aaagatctga caaaatgcca aaattttgaa gaggaaattt agtcggcttc
     1921 caaaattata agtggcagaa attgagcaat tgtcactttt tgacagtaaa tagaaaattt
     1981 tttgaaaaat tttgaaacgc tttattttgt tacttggtca ttttggcacc ataggagtag
     2041 tttttaacat tttccccact ggcgctactt cattttaaac ttttccaaag attttaattt
     2101 ttaattttaa aaaatttcat ataattcgtc tgcgtcttac agtttcaggc ttcaaaaatc
     2161 tattacatat cttatctttg aattcaggga tccttatgca agcgtggaac tgtcgtgagg
     2221 tatggcgccg catacgcaaa caccttgcac acacatcgag aactaccgta tactcgcagt
     2281 ttaaatttca aattttcgaa atttttttcc aatcattttt ccctattttt cttcatttcg
     2341 ctgctaaaaa tagttgtttt gaacccgatt ttgtgcgaaa tcgtggaagt ttacacagct
     2401 tttacagttg aaaatcaaaa aagcgcgctt ttttcgtcta atttgcccag ttttcagctc
     2461 gattcgccgc taccatctga catcacactg cacaatctcg aaccggcaag gcctgattcc
     2521 ggaatgagtt tttccactga ttttgacggt aaattcgtga tttaaagttg taaattccaa
     2581 ttttatttgt ttttttttca gatgatttct tcaatctcga cctccatcaa caagagcgtt
     2641 cggcttcttt tggcggagta acccagtatt ctcaacaatt tcttcgcgaa aaatgctcgt
     2701 tctctccgta tttccacaca tctttagaga ctgttgacag cggaagaact agcctatacg
     2761 ggagcaatga gcaatgtgga cagctcggcg gagcatcttc aaacgggtcg acagcaatgc
     2821 ttcatactcc agatggaagc aattctcatc agacatcgtt tccttcggat ttcagaatgt
     2881 ccgaatcgcc agacgatacc gtatcgggaa aaaagacaac gaccagacgg aacgcttggg
     2941 gaaatatgtc atatgctgaa cttatcacta cagccattat ggctagtcca gagaaacggt
     3001 taactcttgc acaagtttac gaatggatgg tccagaatgt tccatacttc agggataagg
     3061 gagattcgaa cagttcagct ggatggaaga actcgatccg tcacaatctg tctcttcatt
     3121 ctcgtttcat gcgaattcag aatgaaggag ccggaaagag ctcgtggtgg gttattaatc
     3181 cagatgcaaa gccaggaagg aatccacggc gtacacgtga acgatccaat actattgaga
     3241 cgactacaaa ggctcaactc gaaaaatctc gccgcggagc caagaagagg ataaaggaga
     3301 gagcattgat gggctccctt cactcgacac ttaatggaaa ttcgattgcc ggatcgattc
     3361 aaacgatttc tcacgatttg tatgatgatg attcaatgca aggagcattt gataacgttc
     3421 catcgtcttt ccggttagta atttagtttt atttttattt tttgaatttt ccaaatttcc
     3481 aaattttttc agtccccgaa ctcaatcgaa cctctcgatt cctggatcgt cgtctcgtgt
     3541 ttctccagct attggaagtg atatctatga tgatctagaa ttcccatcat gggttggcga
     3601 atcggttcca gcaattccaa gtgatattgt tgatagaact gatcaaatgc gtatcgatgc
     3661 aactactcat attggtggag ttcagattaa gcaggagtcg aagccgatta aggtgagaaa
     3721 tgagaatttg caattttcag gaaaatttga aaattttctt gtggttagta caagtgagct
     3781 cctttttaga caattaaaaa atttgaattt tacttaaatt caatattttt aattctgaat
     3841 ttagtttcaa ggtcccctaa aatattagta acatgcaaat cattttatat tttctcacac
     3901 cgaaaattag aattttcttt ttttttaagt gaaaaaactg agtaatatat agaattttca
     3961 gacggaacca attgctccac caccatcata ccacgagttg aacagtgtcc gtggatcgtg
     4021 tgctcagaat ccacttcttc gaaatccaat tgtgccaagc actaacttca agccgttagt
     4081 ttttttttga acagaagtta attaatctat cgtaatttca caagattttg ggtcccggcg
     4141 ctcaaaaatg ttttttttaa atttttatgt taaaaatagt tttaaatcag ttttgaaagt
     4201 gtaaattttg gcttaaaatt attttaaaca gaaaattgga aataaaacga agaaataaac
     4261 ttttttgcgc gccgagaccc atctgaataa atgcgtgcgc ctttaagaaa cctgtgaaat
     4321 tactatatat acaaaattct gagaatgcgt attacgcaac aaatttgacg cgcaaaatat
     4381 ctcgtagcga aaactacagt aatccttaaa atgagtactg tagcgcttgc gtcgatttac
     4441 gggctcgatt ttcgtgaatt aatttatttt cgaaaagtga cagcgatatt acatttaact
     4501 ttgttctttc taatcatttt ctcatttttg tgaattttta aaaataattt ttcgattaat
     4561 aaaggattgc cgtaaatcga cacatggcgc tacagtaggc atttaaagga ttactgtagt
     4621 tttcgctacg agatatttcg cgcgtcaaat atgttgcgta atacgcattc tcagaatttt
     4681 atgatcccat aatatatatt tcttctttaa ttcaatatat aataattatc caaaatttgt
     4741 tcagaatgcc actaccgggt gcctatggaa actatcaaaa tggtggaata actccaatca
     4801 attggctatc aacatccaac tcatctccac tgcctggaat tcaatcgtgt ggaattgtag
     4861 ctgcacagca tactgtcgct tcttcatcgg ctcttccaat tgatttggaa aatctgacac
     4921 ttcccgatca gccactgatg gatactatgg atgttgatgc attgatcaga catgagctga
     4981 gtcaagctgg agggcagcat attcattttg atttgtaact gcaggaattc gatatgcaga
     5041 agctgatctc agaggaggac ctgcttcaga agcttatctc agaggaggac cttcgacctc
     5101 gagggggggc ccggtaccat ggtattgata tctgagctcc gcatcggccg ctgtcatcag
     5161 atcgccatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac
     5221 atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa aacttcttct
     5281 taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc
     5341 aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa
     5401 taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttttac
     5461 ttctgataaa ttttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata
     5521 tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga
     5581 cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt
     5641 gaaagtttat gaaattaatt ttcctgcttt tgctttttgg gggtttcccc tattgtttgt
     5701 caagagtttc gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc
     5761 aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata
     5821 atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa
     5881 tataccaaac ataactgttt cctactagtg gccacgtggg ccgtgcacct taagcttggc
     5941 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     6001 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     6061 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     6121 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     6181 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6241 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6301 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6361 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     6421 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6481 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     6541 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6601 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     6661 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     6721 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     6781 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     6841 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     6901 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6961 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7021 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7081 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7141 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7201 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     7261 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7321 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7381 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7441 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7501 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     7561 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7621 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7681 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7741 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7801 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7861 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7921 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7981 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8041 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8101 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8161 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     8221 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8281 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8341 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8401 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8461 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8521 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8581 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8641 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8701 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8761 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aga
//
