LOCUS       sequence-34001-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Walker SC, Avis JM, Conn GL
  TITLE     General plasmids for producing RNA in vitro transcripts with 
            homogeneous ends.
  JOURNAL   Nucleic Acids Res. 2003 Aug 1. 31(15):e82.
  PUBMED    12888534
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2003 Aug 1. 31(15):e82."
COMMENT     Sequence Label: p2RZ
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(137..153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(137..153)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(150..172)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    161..177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(185..191,192..209,210..215))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    230..251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(368..385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(619..638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctaatacga ctcactatag ggcccgggat ccgctagcca tggtcccagc ctcctcgctg
       61 gcggctagtg ggcaacatgc ttcggcatgg cgaatgggac tttaaactcg agggatatct
      121 agaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      241 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      721 tggcgctttc tcatagctca cgct
//
