LOCUS       sequence-340013-        9394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian acoustic reporter gene 1 cassette 3 on PB-UBC plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Howells AR, Welch PJ, Kim J, Forest CR, Shi C, Lian XL
  TITLE     A drug-selectable acoustic reporter gene system for human cell 
            ultrasound imaging.
  JOURNAL   Bioeng Transl Med. 2023 Aug 2;9(2):e10584. doi: 10.1002/btm2.10584. 
            eCollection 2024 Mar.
  PUBMED    38435822
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bioeng 
            Transl Med."; date: "2023-08-2"; volume: "9(2):e10584. doi"; pages: 
            " 10.1002/btm2.10584. eCollection 2024 Mar"
COMMENT     Sequence Label: PB-UBC-mARG3
FEATURES             Location/Qualifiers
     source          1..9394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        255..1466
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1303..1322
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1481..1490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1805..1861
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      1988..1997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1992..2001
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2645..2701
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2984..3040
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3866..3922
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    4223..4271
                     /note="synthetic polyadenylation signal"
     protein_bind    4317..4350
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4415..4914
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4450..4473)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4847..4866
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        4926..4973
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4992..5795
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5055..5074)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5665..5684
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5827..5844)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5833..6057
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6114..6147
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   6344..6406
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6457..6477)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6457..6475)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6496..6512)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6496..6512)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6509..6531)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6520..6536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6544..6550,6551..6568,6569..6574))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6589..6610
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6727..6744)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6898..7486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6978..6997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7657..8448,8449..8517))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8280..8299
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8518..8622)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8648..9103)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8785..8806)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8997..9016
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9230..9252
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9244..9261
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9245..9261
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9271..9290
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9271..9289
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   9319..9353
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ccgtcgctgt gcatttagga catctcagtc gccgcttgga gctcccgtga ggcgtgcttg
       61 tcaatgcggt aagtgtcact gattttgaac tataacgacc gcgtgagtca aaatgacgca
      121 tgattatctt ttacgtgact tttaagattt aactcatacg ataattatat tgttatttca
      181 tgttctactt acgtgataac ttattatata tatattttct tgttatagat atcaactaga
      241 atgctagcct cgagggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
      301 cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg
      361 gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca
      421 gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag
      481 cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg
      541 cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca
      601 gccgggattt gggtcgcagt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag
      661 cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaggcg
      721 tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg
      781 ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt
      841 gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa
      901 ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac
      961 catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt
     1021 gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc
     1081 gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg tggggccacc
     1141 tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg ggcctagggt
     1201 aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa gtgaggcgtc
     1261 agtttctctg gtcggtttta tgtacctatc ttcttaagta gctgaagctc cggttttgaa
     1321 ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt
     1381 aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct
     1441 ggccgttttt ggcttttttg ttagacggat ccatgaattc gccaccatgg ccgtggaaca
     1501 caacatgcag agcagcacca tcgtggacgt gctggaaaag atcctggaca agggcgtcgt
     1561 gatcgccggg gacatcacag tgggaatcgc cgacgtggaa ctgctgacca tcaagatccg
     1621 gctgatcgtg gccagcgtgg acaaggccaa agaaatcggc atggattggt gggagaacga
     1681 cccctacctg agcagcaagg gcgccaacaa caaggccctg gaagaggaaa acaagatgct
     1741 gcacgagcgg ctgaaaacac tggaagagaa gatcgagaca aagcgcggtg ccccgggatc
     1801 tggcgcaaca aattttagtc ttttaaagca ggcaggagac gtcgaggaaa accctggacc
     1861 cgtgagcgag acaaacgaga caggcatcta catcttcagc gccatccaga cagacaagga
     1921 tgaggaattc ggcgccgtgg aagtggaagg gaccaaggct gagacattcc tgatccggta
     1981 taaggacgcc gccatggtgg ccgccgaagt gcccatgaag atctaccacc ccaaccggca
     2041 gaacctgctg atgcaccaga atgccgtggc cgccatcatg gacaagaacg acaccgtgat
     2101 ccccatcagc ttcggcaacg tgttcaagag caaagaggac gtgaaggtgc tcctggaaaa
     2161 cctgtacccc cagttcgaga agctgttccc cgccatcaag ggaaagatcg aagtgggcct
     2221 gaaggtgatc ggcaagaaag agtggctcga aaagaaagtg aacgagaacc ccgagctgga
     2281 aaaagtgtcc gccagcgtga agggcaagag cgaggccgct ggctactacg agagaatcca
     2341 gctgggcggc atggcccaga agatgttcac aagcctgcag aaagaagtga aaaccgacgt
     2401 gttcagcccc ctggaagaag ccgccgaggc cgccaaagcc aatgagccta caggcgaaac
     2461 aatgctgctg aacgccagct tcctgatcaa cagagaggat gaggccaagt tcgacgagaa
     2521 agtcaatgag gcccacgaga actggaagga taaggccgac ttccactaca gcggcccctg
     2581 gcccgcctac aacttcgtga acatccggct gaaggtggaa gagaaggggg cacctggctc
     2641 gggagcgacc aacttctcat tactcaaaca agccggagac gttgaggaga atccaggccc
     2701 tgtgctgcac aagctcgtga ccgcccccat caacctggtc gtgaagatcg gcgagaaggt
     2761 gcaggaagag gccgacaagc agctgtacga cctgcccacc atccagcaga agctgatcca
     2821 gctgcagatg atgttcgagc tgggcgagat ccccgaggaa gccttccagg aaaaagagga
     2881 cgaactgctg atgagatacg agatcgccaa gcggcgcgag attgagcagt gggaagaact
     2941 gacccagaag cggaatgagg aaagcggtgc cccgggatct ggcgcaacaa attttagtct
     3001 tttaaagcag gcaggagacg tcgaggaaaa ccctggaccc gtgggcgagc tgctgtacct
     3061 ctacggcctg atccccacca aagaggccgc tgctatcgag cccttcccat tctacaaggg
     3121 cttcgacggc gagcacagcc tgtaccctat cgccttcgac caagtgaccg ccgtggtgtt
     3181 caagctggac gccgacacct acagcgagaa agtgatccag gaaaagatgg aacaggacat
     3241 gagctggctg caggaaaagg ccttccacca ccacgagaca gtggccgccc tgtatgagga
     3301 attcaccatc atccccctga agttctgcac catctataag ggagaggaat ccctgcaggc
     3361 cgccatcgag atcaacaaag agaagatcga aaactccctg accctgctgc agggcaacga
     3421 ggaatggaac gtgaagatct actgcgacga caccgagctg aagaagggca tcagcgagac
     3481 aaacgagagc gtgaaggcca agaagcagga aatcagccac ctgagccccg gcagacagtt
     3541 cttcgagaag aagaagattg accagctcat cgagaaagag ctggaactgc acaagaacaa
     3601 agtgtgcgag gaaatccacg acaagctgat tgagctgagc ctctacgact ccgtgaagaa
     3661 gaactggtcc aaggacgtga caggcgctgc cgaacagatg gcctggaaca gcgtgttcct
     3721 gctgcccagc ctgcagatca ccaagttcgt gaacgagatc gaggaactcc agcagcggct
     3781 ggagaacaag ggatggaagt tcgaagtgac cggcccctgg cctccctacc acttcagcag
     3841 ctttgccggg gcacctggct cgggagcgac caacttctca ttactcaaac aagccggaga
     3901 cgttgaggag aatccaggcc ctgtgcagcc cgtgtcccag gccaacggca gaatccacct
     3961 ggatcccgat caggccgaac agggactggc ccagctcgtg atgaccgtga tcgagctgct
     4021 gcggcagatc gtggaacggc acgccatgag aagagtggaa ggcggcaccc tgaccgacga
     4081 gcagatcgag aatctgggaa tcgccctgat gaacctggaa gagaagatgg acgagctgaa
     4141 agaggtgttc ggactggacg ccgaggacct gaacatcgac ctgggccctc tgggcagcct
     4201 gctgtgaaag cttcatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
     4261 ttttttgtgt gtcaattcct gcagcccaat tccgatcata ttcaataacc cttaatataa
     4321 cttcgtataa tgtatgctat acgaagttat taggtctgaa gaggagttta cgtccagcca
     4381 agctagcttg gctgcaggtc gtcgaaattc taccgggtag gggaggcgct tttcccaagg
     4441 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     4501 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     4561 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     4621 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     4681 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     4741 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     4801 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     4861 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     4921 agcctgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     4981 ggaactaaac catgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg
     5041 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     5101 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     5161 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     5221 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     5281 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     5341 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     5401 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     5461 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     5521 tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc
     5581 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     5641 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     5701 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5761 tcgccttcta tcgccttctt gacgagttct tctgagggga tcaattctct agagctcgct
     5821 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     5881 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     5941 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     6001 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     6061 ctgaggcgga aagaaccagc tggggctcga ctagagcttg cggaaccctt aatataactt
     6121 cgtataatgt atgctatacg aagttattag gtccctcgac atactagtta aaagttttgt
     6181 tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata aacataaata
     6241 aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta
     6301 ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt caattttacg
     6361 catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa tctagtatac
     6421 gcgtatgcgg ccgcttaatt aatccagctt ttgttccctt tagtgagggt taattgcgcg
     6481 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6541 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6601 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6661 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6721 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6781 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6841 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6901 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6961 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7021 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7081 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7141 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7201 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7261 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7321 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7381 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7441 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7501 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7561 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7621 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7681 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7741 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7801 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7861 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7921 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7981 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8041 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8101 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8161 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8221 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8281 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8341 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8401 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8461 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8521 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8641 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     8701 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     8761 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8821 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8881 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8941 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     9001 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     9061 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     9121 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     9181 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     9241 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggg
     9301 cgcgccattc tagattaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt
     9361 gaaatattgc tctctctttc taaatagcgc gaat
//
