LOCUS       sequence-340019-        2086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 1a of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 1a Spacer
FEATURES             Location/Qualifiers
     source          1..2086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(450..484)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(555..1214)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1129..1148
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1396..1984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1885..1904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggcacctgc cacctgacat gttcgtgttc ctggtgctcc tgcctctggt gagcagccag
       61 tgcgtgaacc tgaccacccg aacccagctc ccaccagcct acaccaacag ctttacacgg
      121 ggcgtgtact accctgacaa ggtgttcaga tctagcgtcc tgcacagcac tcaggacctc
      181 ttcctgccgt tcttcagcaa cgtgacatgg ttccacgcca tccacgtgag cggcacaaac
      241 ggaaccaagc ggtttgataa ccccgtcctg ccattcaatg atggagttta cttcgccagt
      301 accgagaaga gtaacatcat ccggggctgg atcttcggca ccaccctgga tagcaaaaca
      361 cagagcctcc tgatcgtgaa caatgccacg aacgtcgtga tcaaggtgtg cgagttccag
      421 ttttgcaagg tggcaggtgg accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc
      481 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
      541 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      601 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
      661 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
      721 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa
      781 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
      841 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
      901 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
      961 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     1021 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1081 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1141 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1201 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1261 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtagt
//
