LOCUS       sequence-340020-        2067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 1b of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 1b Spacer
FEATURES             Location/Qualifiers
     source          1..2067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(479..513)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(584..1243)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1158..1177
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1425..2013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1914..1933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtagtcg gcacctgcca
       61 ccgcaatgat cctttcctgg gtgtgtacta ccacaagaac aacaagagct ggatggaaag
      121 cgagttcaga gtctacagca gcgccaacaa ctgcacattc gagtacgtct ctcagccttt
      181 tctgatggac cttgagggga aacaaggcaa cttcaagaac ctgagagaat tcgtgttcaa
      241 gaacatcgac ggctacttca aaatctactc caagcacaca cccatcaacc tggtccggga
      301 cctccctcag ggcttcagcg ccctggaacc cctggtcgac ctgcccatag gcatcaacat
      361 aacgcggttc caaaccctgc tggccctgca tagatcctac ctgactcctg gcgacagcag
      421 cagcggatgg accgccggag ctgcagccta ctatgtgggt ggcaggtgga ccagaccaat
      481 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
      541 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
      601 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
      661 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
      721 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
      781 tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag
      841 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
      901 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
      961 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     1021 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     1081 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     1141 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     1201 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     1261 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     1321 agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg
     1381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1561 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2041 ttcctggcct tttgctggcc ttttgct
//
