LOCUS       sequence-340024-        2473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 3 of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 3 Spacer
FEATURES             Location/Qualifiers
     source          1..2473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(749..783)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(854..1513)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1428..1447
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1695..2283
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2184..2203
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaccgggacc ggcgtgctga ccgaaagcaa caaaaagttc ctccccttcc agcagttcgg
       61 ccgtgatatc gctgacacca cagatgccgt cagagatcca cagaccctgg aaatcctgga
      121 tattacaccc tgctccttcg gaggagtttc tgtgatcacc cccgggacca ataccagcaa
      181 ccaggtggct gtgctgtacc aaggtgttaa ctgcaccgag gttcctgtgg ccatccacgc
      241 cgatcagctg acacctactt ggagagtgta ctccactggc tccaatgtgt tccagaccag
      301 ggccggatgt ctgatcggcg ccgagcacgt gaataacagt tacgagtgcg acatccctat
      361 cggcgccggc atctgtgcca gctaccagac ccagacaaac agccctgggt ctgcttcctc
      421 tgtagctagc cagagcatca tcgcctacac catgagcctg ggcgcagaga acagcgtggc
      481 ctattccaac aactctatcg ccattcccac caactttaca attagcgtca caacagagat
      541 cctgcccgtg agcatgacca agaccagcgt ggactgtaca atgtacatct gtggcgacag
      601 cactgaatgc agcaacctgc tgctgcaata cggctccttt tgcacccaac tgaaccgggc
      661 gctgaccgga atcgccgtgg aacaggacaa aaatacccag gaggtgttcg cccaagtgaa
      721 gcagatcggt ggcaggtgga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct
      781 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
      841 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
      901 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
      961 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1021 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa
     1081 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1141 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1201 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1261 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     1321 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1381 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1441 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1501 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1561 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc
     1621 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1681 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1741 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1801 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1861 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1921 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1981 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2041 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2101 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2161 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2221 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2281 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2341 atgttctttc ctgcgttatc ccctgattct gtggataacc gtagtcggca cctgccaccg
     2401 ccaccgtctg cggtcctaag aagtccacca atctggttaa gaacaaatgc gtgaacttca
     2461 acttcaacgg cct
//
