LOCUS       sequence-340030-        2137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Part 5 of HexaPro-D614G spike to be used in the Spike 
            Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 5 Spacer
FEATURES             Location/Qualifiers
     source          1..2137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(530..564)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(635..1294)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1209..1228
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1476..2064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1965..1984
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcccctga ttctgtggat aaccgtagtc ggcacctgcc acccagagcg cgccgcacgg
       61 cgtggtgttc ctgcatgtga catacgtgcc tgcccaagag aagaatttca caaccgcccc
      121 tgccatctgc cacgacggca aggcccactt cccaagagag ggcgttttcg tttccaatgg
      181 cacacactgg ttcgtgacac aaagaaactt ctacgaaccc cagattatca ccaccgacaa
      241 caccttcgtg agtggcaatt gtgacgtggt catcggaatc gtgaacaaca cagtgtacga
      301 ccctctgcaa cctgagctgg actcttttaa ggaagagctg gacaagtact ttaaaaacca
      361 caccagcccc gatgtggacc tgggcgacat cagtggcatt aacgccagcg tggtgaacat
      421 ccaaaaggaa atcgacagac tgaacgaggt ggccaagaac ctgaacgagt ccctgatcga
      481 cctgcaggag ctcggcaaat acgagcaggg tggcaggtgg accagaccaa taaaaaacgc
      541 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
      601 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
      661 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
      721 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
      781 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
      841 tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg
      901 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
      961 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     1021 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     1081 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     1141 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     1201 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     1261 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     1321 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     1381 ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc gttccactga
     1441 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1501 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1561 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1621 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1681 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1741 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1801 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1861 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1921 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1981 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2041 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2101 ttttgctggc cttttgctca catgttcttt cctgcgt
//
