LOCUS       sequence-340093-        2562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ribonuclease A or RNase A; endoribonuclease purified from bovine 
            pancreas. Important enzyme for the removal of RNA for RNA free DNA 
            purification reactions such as plasmid DNA purification and genomic 
            DNA purification, RNA removal from recombinant protein preparations,
            ribonuclease protection assays, mapping single-base mutations in 
            DNA/RNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2562)
  TITLE     Open Enzyme Collection
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOpen-RNAse A
FEATURES             Location/Qualifiers
     source          1..2562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             107..151
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(585..603)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      599..646
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(651..667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(651..667)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(734..1322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(814..833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1513..2304,2305..2373))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2136..2155
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2374..2478)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2494..2510
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2511..2542
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aaaggggccg tcaatatcag ggtctctaat ggcactgaaa tctctcgtgc tgctctctct
       61 gctggtactg gttctgctgc tggttcgcgt gcagccgtcg ctgggcaagg aaaccgctgc
      121 ggccaaattc gaacgtcagc acatggactc gtctacctct gccgcatcct ctagcaacta
      181 ctgcaaccaa atgatgaaat ctcgcaacct gaccaaggat cgttgcaaac cggttaacac
      241 cttcgtgcat gaatccctgg cagacgtcca ggctgtttgc tcccagaaga acgtagcatg
      301 caagaacggc cagaccaact gttatcagtc ttattcgacg atgagcatca ccgattgccg
      361 tgaaactggt tcctcaaaat atcctaactg cgcgtataaa acaactcagg ccaacaaaca
      421 tattatcgtg gcttgcgaag gcaaccctta cgtgccggtg cactttgatg cttctgtttg
      481 aagctcaatc taacagtggg gcacgaggct cgccgttgct attagggata acagggtaat
      541 ggccatagag agtgaaatta acgctgaggt gtcaatcgtc ggagccgctg agcaataact
      601 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgcatg gtcatagctg
      661 tttcctgaga gcttggcagg tgatgacaca cattaacaaa tttcgtgagg agtctccaga
      721 agaatgccat taatttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1141 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1261 ctggtagcgg tggttttttt gtttgcaagc agcagattac acgcagaaaa aaaggatctc
     1321 aagaaggcct actattagca acaacgatcc tttgatcttt tctacggggt ctgacgctca
     1381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1501 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1561 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1621 accatctggc cccagtgctg caatgatacc gcgagaacca cgctcaccgg ctccagattt
     1681 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1741 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1801 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1861 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1921 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1981 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2041 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2101 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2161 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2221 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2281 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2341 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2401 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2461 acaaataggg gttccgcgca cctgcaccag tcagtaaaac gacggccagt agtcaaaagc
     2521 ctccgaccgg aggcttttga cttggttcag gtggagtggg ag
//
