LOCUS       sequence-340094-        4628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA polymerase that efficiently reverse-transcribes RNA in the 
            presence of MnCl2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4628)
  TITLE     Open Enzyme Collection
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOpen-TthRT
FEATURES             Location/Qualifiers
     source          1..4628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2651..2669)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2665..2712
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2717..2733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2717..2733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(2800..3388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2880..2899)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3579..4370,4371..4439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4202..4221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4440..4544)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4560..4576
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4577..4608
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aaaggggccg tcaatatcag ggtctctaat ggaagctatg ctgccgctgt tcgaaccgaa
       61 aggccgtgta cttctggttg acggccatca tctggcatat cgtaccttct ttgcgctgaa
      121 aggcctgacg actagccgcg gtgaaccagt tcaggctgta tacggtttcg caaaatctct
      181 gctgaaggca ctgaaagagg acggctataa agctgtgttc gtggttttcg acgcaaaagc
      241 gccgtctttc cgtcacgaag cgtacgaggc ttataaagcg ggccgtgctc ctaccccgga
      301 agatttcccg cgtcagctgg cgctcattaa agaactggtt gacctcctgg gattcactcg
      361 cctggaagtt ccgggttacg aggctgatga tgtcctggcg accctggcta aaaaagccga
      421 aaaagaagga tacgaagttc gtattctgac cgctgaccgt gatctgtacc agctggtaag
      481 cgatcgtgtt gcggtgctgc accctgaagg tcatctgatc actccagaat ggctgtggga
      541 aaaatacggt ctgcgcccgg agcagtgggt agacttccgc gctctggtcg gggacccgtc
      601 tgacaacctg ccgggcgtga aaggtatcgg ggaaaagacc gcactgaaac tgctgaaaga
      661 atggggcagc ctggaaaacc tgctgaaaaa cctggaccgt gtaaaacctg aaaacgttcg
      721 tgagaaaatc aaagctcatc tggaggatct gagacttagc ctggaactgt cccgtgttcg
      781 tacggatctg ccactggaag ttgatctggc ccagggtcgt gaaccggatc gtgaaggttt
      841 acgcgctttt ctggagcgtc tggaatttgg tagcttgctg catgaatttg gcctgctcga
      901 agcgccagcg ccgctggaag aagcaccgtg gccgccgcct gaaggtgctt ttgttggttt
      961 cgttctgtcc cgcccggaac cgatgtgggc ggaactgaaa gccctggcgg cgtgccgtga
     1021 cggccgcgtc caccgtgcag cagacccgct cgcgggcctg aaagacttga aggaagtccg
     1081 cggtttgctg gccaaagacc tcgccgttct ggccagccgt gaaggcctgg atctggttcc
     1141 gggtgatgac ccgatgcttc tggcctacct gcttgacccg tccaatacca ccccggaagg
     1201 cgtggctcgc cgttacggtg gcgaatggac ggaggacgcc gcgcaccgtg cgctgctgtc
     1261 tgagcgtttg caccgtaacc tcctgaaacg tctggaaggt gaagaaaaac tgttgtggct
     1321 gtatcacgaa gttgaaaagc cgctgtcccg cgtgctggcg cacatggaag ctactggtgt
     1381 acgtcgtgac gtagcctatc tgcaagccct gtctctggaa ctggcagaag aaattcgtcg
     1441 cctggaagaa gaagtttttc gcctggctgg tcacccgttt aacttgaact ctcgtgatca
     1501 gctggaacgc gttttgtttg atgaactgcg cttgccggcc ctgggcaaaa cccagaaaac
     1561 cggtaaacgt tctacgtctg ccgctgtgtt ggaagcgctg cgtgaagcgc atccgattgt
     1621 agaaaaaatc ctgcaacacc gcgaactgac caagctgaag aacacgtacg tcgacccgct
     1681 gccgagcctg gttcatccgc gtaccggtcg tctgcacact cgcttcaacc agaccgcgac
     1741 cgctaccggt cgcctgagct cgtccgatcc gaacctgcaa aatattccgg tgcgtacccc
     1801 gctgggtcag cgcatccgtc gtgcgttcgt cgccgaggct ggttgggctc tggtagcact
     1861 ggactatagt cagatcgagc tgcgtgttct ggctcacctc agcggcgacg aaaacctgat
     1921 ccgtgtgttc caggaaggca aggatatcca cactcagacc gcgagctgga tgttcggtgt
     1981 tccaccggaa gccgttgacc cgctgatgcg tcgtgcagct aaaactgtta actttggcgt
     2041 actgtacggt atgtccgcgc accgtctgag ccaggaactg gcaatcccgt atgaagaagc
     2101 tgtggcgttc attgaacgct atttccagag ctttccgaaa gttcgtgcct ggattgaaaa
     2161 aacgctggag gaaggacgta agcgtggtta tgttgaaaca ctgttcggtc gccgtcgtta
     2221 cgtaccggat ctgaacgcac gcgtcaaatc cgtgcgtgaa gcagcggaac gtatggcgtt
     2281 caacatgccg gtgcagggta ccgcggccga tctgatgaaa ctggcaatgg ttaaactgtt
     2341 cccgcgtctg cgtgagatgg gtgctcgtat gctgttgcag gttcacgacg aactgctgct
     2401 ggaggcgcca caggcacgcg cagaagaagt tgcagcgctg gcgaaagaag ctatggaaaa
     2461 agcatacccg ctggcggttc cgctggaggt ggaagtggga atgggtgaag attggttgtc
     2521 tgcgaaaggc tgaagagctt tgagaccagt attcttacag ccagccgcgt cgacggctag
     2581 tcgagggtta gggataacag ggtaatgatg tgggtgagtc gaccttgcgc tgaggtgtca
     2641 atcgtcggag ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     2701 aggggttttt tgcatggtca tagctgtttc ctgagagctt ggcaggtgat gacacacatt
     2761 aacaaatttc gtgaggagtc tccagaagaa tgccattaat ttccataggc tccgcccccc
     2821 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2881 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2941 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3001 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3061 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3241 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3361 gattacacgc agaaaaaaag gatctcaaga aggcctacta ttagcaacaa cgatcctttg
     3421 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3481 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3661 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3721 gaaccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3781 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3841 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3901 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3961 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4021 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4081 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4141 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4201 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4261 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4321 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4381 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4441 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4501 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacctg caccagtcag
     4561 taaaacgacg gccagtagtc aaaagcctcc gaccggaggc ttttgacttg gttcaggtgg
     4621 agtgggag
//
