LOCUS       sequence-340101-       14795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes OptoQ-AMP5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14795)
  AUTHORS   Lalwani MA, Zhao EM, Wegner SA, Avalos JL
  TITLE     The Neurospora crassa Inducible Q System Enables Simultaneous 
            Optogenetic Amplification and Inversion in Saccharomyces cerevisiae 
            for Bidirectional Control of Gene Expression.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):2060-2075. doi: 
            10.1021/acssynbio.1c00229. Epub 2021 Aug 4.
  PUBMED    34346207
REFERENCE   2  (bases 1 to 14795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00229"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "2060-2075"
COMMENT     Sequence Label: pMAL724
FEATURES             Location/Qualifiers
     source          1..14795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(103..134)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     terminator      complement(305..552)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     518..536
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(1441..1461)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(1491..1888)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(2551..2570)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2552..2570)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2577..2594)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2577..2593)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2586..2608)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2734..3189
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2821..2840)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3031..3052
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3545..3564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3664..3686
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3724..3742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3826..3930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3931..3999,4000..4791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4149..4168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4962..5550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5451..5470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5704..5721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5838..5859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5874..5879,5880..5897,5898..5904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5912..5928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5917..5939
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5936..5952
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5936..5952
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5973..5991
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6317..6504
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     terminator      complement(12243..12408)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     12329..12353
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(12424..13131)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(12464..12484)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     13079..13107
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctgcatgggc gttagcttcc gctgccgcgt tcagcgtctt tctcttgggt ggcatgattt
       61 tagcggccgc ggtggcttta ccaacagtac cggattgcca agctggaagt cgagcttcca
      121 ttatataccc tctagtgtct cttaagcatg gactaaaggc tacctataac gcatggacta
      181 aaggctacct ataacgcatg gactaaaggc tacctataac gcatggacta aaggctacct
      241 ataacgcatg gactaaaggc tacctactcg aagctggcga actcacgtac ccggcgataa
      301 ggccgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt ttcagtataa
      361 tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat ataaataacg
      421 ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca ggttgtctaa
      481 ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag cgtgacataa
      541 ctaattacat gatcattact cgaggtcgac ttaaatacct gcttctacgg caattctaac
      601 taaatctgca ctcgttttta agtttaattt ttccattacc aaacctctgt gcattttgac
      661 agttttttct gaaagaccca gtctggcagc cacttcttta tttcttaggc ctgatgcaac
      721 cagagttgtg acttctaatt gacgtggtga aagagttttt aacatctccg cagcacgttc
      781 tctacgggcc atgcccatgt tcggttgatc gtcatccact tccacttggc taccaaggaa
      841 gtatagtaat tcatcatcgt catcatagat tggtgccacc aaaactgcat tacgaaaagg
      901 agtaccatcc tttttgtaat tcaaaatctc aaccaacacg ggtttatgct cacgaacacc
      961 ttgacggatc ttatctgtta gccagggttc agtaccagat ccctgtaaga acctacaatt
     1021 acgcccaaca cattcttcct cagaataccc agttagatcg gtaaaggcct ggttgatagc
     1081 aatcaaagga ttatcagcca atctgggatc actaacaaca cttgctatgg gtgaggcctc
     1141 gatcaaatcc aatacccatt gggctggagg ttggacttca actctagtat catctgctcc
     1201 gaattcacct ccatattcgt cgataccaag tgcatcagta aacatctgtt caaattcgaa
     1261 atctgccatg tccaacgcgc catacggagc agaatcatgt ggtgtaaatc cggggccggg
     1321 agaatcccca tcacctaaca tgtccagatc aaaatcatca agagcatcgg catgtgccat
     1381 agctacatcc tcaccgtcta aatgtaattc gtcaccaagt gaaacgtccg ttggtggggc
     1441 tacttttctc ttttttttcg ggctagccat gattttagcg gccgccaaaa cttagattag
     1501 attgctatgc tttctttcta atgagcaaga agtaaaaaaa gttgtaatag aacaagaaaa
     1561 atgaaactga aacttgagaa attgaagacc gtttattaac ttaaatatca atgggaggtc
     1621 atcgaaagag aaaaaaatca aaaaaaaaat tttcaagaaa aagaaacgtg ataaaaattt
     1681 ttattgcctt tttcgacgaa gaaaaagaaa cgaggcggtc tcttttttct tttccaaacc
     1741 tttagtacgg gtaattaacg acaccctaga ggaagaaaga ggggaaattt agtatgctgt
     1801 gcttgggtgt tttgaagtgg tacggcgatg cgcggagtcc gagaaaatct ggaagagtaa
     1861 aaaaggagta gaaacatttt gaagctatca gctgtttccc gggagctgga actcccacct
     1921 gcaacatgcg ggatcctgag taaataagcg gccgccaccg cggtggagct cagatcttga
     1981 caccgattat ttaaagctgc agcatacgat atatatacat gtgtatatat gtatacctat
     2041 gaatgtcagt aagtatgtat acgaacagta tgatactgaa gatgacaagg taatgcatca
     2101 ttctatacgt gtcattctga acgaggcgcg ctttcctttt ttctttttgc tttttctttt
     2161 tttttctctt gaactccaga aaaaaaatat aaaagagatg gaggaacggg aaaaagttag
     2221 ttgtggtgat aggtggcaag tggtattccg taagaacaac aagaaaagca tttcatatta
     2281 tggctgaact gagcgaacaa gtgcaaaatt taagcatcaa cgacaacaac gagaatggtt
     2341 atgttcctcc tcacttaaga ggaaaaccaa gaagtgccag aaataacagt agcaactaca
     2401 ataacaacaa cggcggctac aacggtggcc gtggcggtgg cagcttcttt agcaacaacc
     2461 gtcgtggtgg ttacggcaac ggtggtttct tcggtggaaa caacggtggc agcggccggc
     2521 catttaaatg tttaaacggc cacgctggcc ccctatagtg agtcgtatta caattcactg
     2581 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     2641 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2701 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
     2761 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2821 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2881 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2941 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3001 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3061 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3121 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     3181 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     3241 agggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt attaggatct
     3301 ctacccttgg cgaaaagtcc tctgccaaca atgatgatat ctgatccacc acttacaact
     3361 tcgtcgaggg ttctgtactg ctgacccaat atgcgtatat ataccaatct aagtctgtgc
     3421 tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt tcaaggaaac
     3481 cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaaggtggta tggtgcactc
     3541 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     3601 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     3661 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     3721 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     3781 cgtcacgcgc cgaggacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3841 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3901 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3961 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4021 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4081 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4141 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4201 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4261 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4321 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4381 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4441 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4501 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4561 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4621 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4681 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4741 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4801 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4861 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5101 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5581 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5641 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5701 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     5761 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     5821 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     5881 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     5941 aacagctatg accatgatta cgccaagctc ggaattaacc ctcactaaag ggaacaaaag
     6001 ctgggccggc catttaaatg tttaaacggc cacgctggcc gctgcacggt cctgttccct
     6061 agcatgtacg tgagcgtatt tccttttaaa ccacgacgct ttgtcttcat tcaacgtttc
     6121 ccattgtttt tttctactat tgctttgctg tgggaaaaac ttatcgaaag atgacgactt
     6181 tttcttaatt ctcgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
     6241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
     6301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
     6361 tttttttttt ccacctagcg gatgactctt tttttttctt agcgattggc attatcacat
     6421 aatgaattat acattatata aagtaatgtg atttcttcga agaatatact aaaaaatgag
     6481 caggcaagat aaacgaaggc aaagaggctc tatatgtata taaagctgcg gatgccttct
     6541 gatttaatct agttctagtt tacttcaggt ttacttaaag ttgagataat tgaagcaaag
     6601 agaagcatcg agcaaaggat ggcgtttgtt aagagggtta cgcaggagac gaatatacag
     6661 ctggcgctgg atcttgacgg tgggtctgtt tctgtacggg agagcatact gggcaaggaa
     6721 tatgctagtg gtgatgggca gaccatccat gtgcacactg gagttgggtt tttggaccac
     6781 atgttgactg cgctggcgaa gcatggcggg tggtctctga tcctggagtg tataggggac
     6841 ttgcacatcg atgaccacca cactgttgag gactgtggga ttgcgctggg ccaagcgttc
     6901 aaggaggcgc ttggctccgt gcgtggtatc aagaggttcg ggcatgggtt tgcaccactg
     6961 gacgaggcgc tgagccgcgc tgtggttgac ttctccaata ggcctttcgc cgtggtggag
     7021 ctgggcctga agcgagaacg cataggccag ctatccacag agatgatccc gcacttcttg
     7081 gagagtttcg ccactgaggc gcgtatcacc atgcatgtgg actgtctgcg gggcaccaac
     7141 gaccaccacc gctccgaatc agctttcaag gcgctcgcca tcgccatcag agaggcaaga
     7201 acacctacgg gtcgcgatga cgttccatcc actaagggtg tcctagcata gaacacagcc
     7261 cacagctacc accatcaaca atatttatat agataacgta cacatagaaa tcacacaaac
     7321 agagtattta ttcttaacta catgaactac catcagaccg tctgggccac tatataatgt
     7381 gctattcata aacgtgatca ctctacgtag caggcgaccc caggtgaaaa tttttcagcg
     7441 agctgccaga ttgtcaggtg aaaaactgaa aaaaacttct gggcgatgag cttgtggcgg
     7501 gaaattaagt atataaagca gttagtttct tctgcttctt gtggttctgg gtattcttgt
     7561 aatatttgga ataatagaaa accaagacta ctgcctttct tttcatatta tagaggaaga
     7621 aatacgcacg aacacgatat agaggtaaat tttttgcaaa aagataccaa actataaaag
     7681 aaatagaaga ctaaactaca aaaaataaag aaagtaatac atatatccaa tatgactgag
     7741 ttagcagagc aagtttccat gcctgaaggg caacaaagac gtggtggtta cgttccacct
     7801 cacatccgta acggtggtgg cagaagaggc gattctgctc ctcgtggtgg tttcaacaac
     7861 ggtggcggcc gtggtggttt cttcagaaac aacggtgacc gtggtggatt cggctttggg
     7921 tacctccgga tatgttggcg cgccttaatt tattcgccgg cgatacatgg gtgaccaaaa
     7981 gagcgggcgg atacacgcgt caccacaagc agaataaaag gtaaacctga aattgtttta
     8041 acataaaatg aaaaatgctt gtttgcaacc ctatatagaa tcataaaaca ttcgtgacta
     8101 taaaatgaat aaactaaact attctaagaa aatgaaataa atgacaaaaa aacgtgtttt
     8161 ttggactaga aggcttaatc aaaagctctc gatcattatt attggaagta caagttttca
     8221 gaaccagcct tcttgtacaa agaagtaata gattcttgtg cacaagacat aggaagttct
     8281 cgaaccgctt catcgatatt cacagctgtt tttttcacca ggtcttctcc ctctttcact
     8341 gcggtttctt caatgacatt gcgaactggt tctacgtgct tgctcccgtc tagttgaact
     8401 ccaataaagt attgtacttc tcccttctga tcacgcatag gttgcaagtg gaaaatgttc
     8461 cagaacttct ttccgctctt ggtgtagttg atgagctgaa ctgtcacttc ggtttggtta
     8521 tcaatagcat ttcgaatctt cttcactgtg gttagatcag tctctggacc ttgtagaaac
     8581 ctgcaatttc ttccaagaat ttcttcacgg ctatattccg tgagctccaa gaaactatca
     8641 gatgcaaaaa taatgggatt atcgggaagc ctaggatcag tgataataaa attcttctcg
     8701 atacgttcga gtgtagtagc tagatcaata ccctttctca tctgtcctcc agcactctcg
     8761 agagagattt gcgtggctat cccgtttaga cggggctgta gttgtgccgt gtgactttta
     8821 gcctggtcgt ctggatacgc acgtagcact tcacgcctca tcaacctacg cggcgcacgt
     8881 ctacctgtaa acagttcgaa ctgggcaaag ccttgttcag gcagtaagtc aagtccgtcc
     8941 atggccaccc agccacggtg agcttcacga cgtgtctgct ctagcagtgg ggaggttaag
     9001 cacttataat ctaactctag aactataccg ccggtaggat tgtcaagcca gtgtaggggg
     9061 actgtaaatt ccgggtccgg tgtgttatcg actgtatggg ttggaataca gctcacgatc
     9121 atagtaggat ttttgaaatt ctctggcaat gggtcatctc tagaacggat gatatgaaag
     9181 cacgtattat catgactcct gggccccgtg cttaacaatg gaagtgcatc acgaactaac
     9241 aggttttcga aatgaagaac cagtttttca gcattagcga aagtacggtt gaaaattaaa
     9301 atttttttga ccccaagttg cagcattgca tacactgcgg ctctcgccat ccccccggca
     9361 cctatcacta aaccggtgct tgtagacctc actgcgtttg ctgggctaag cccccttctt
     9421 aaacatgctc ttattccaat ccaatcagta ttttcaccat aaagagctct cactgctccc
     9481 gcctgagaaa tgttattaaa catactgact tcatctggaa tgcccccgtc cgcagtaaga
     9541 tgacgcactg ggattaacgt attcacggct ccgatagcct ttgcgtgtct agacagacta
     9601 tgtgtaagtg aaataatctc aactttgaac ggtaacccta ctgaagctcc agcaaattgt
     9661 ggatcactaa ttacttccct caaagtccct atattagcag ttgatagcgg tttatagtgg
     9721 tgtggtattc cgcaagcttt tagcgctgcg ttgtgcatag ccggtgaaag ggagtagcca
     9781 acgttagccc cgaacacata taatttcata ggatcgtgca caaacgcgga ataaagggct
     9841 tgtgtggctt ccagtgctgt tagaggaggc tgtaagtatc tgtgagccgg attacgaagg
     9901 cctatcgcat cctccttcgt cggtactggg gtgacggggg tcaggatctc attaaagcac
     9961 atagacgttc ttcccaatct cccggtgtta taagcgataa atggcagcct aggtccccca
    10021 accgcctcaa ctgcaacatg gaactgtcta atatctgtgt tatcacgggg attgctagcg
    10081 ggtctggtca acctcactaa atcacaccct gtgttttgtg ctttctcgta tgcttgtaac
    10141 caactcggat caccccatct cggggagttt acctctgcaa gttgtttatt tcctatcact
    10201 ttcgtggtcc cttggacggt tgttagctgt cctaacaaac cgctgtcaag cccaaggtct
    10261 acggtcaggt agtcgggtgc aagcctaagt gcatggttaa gataagtcat gtacaacgct
    10321 agaagggctt cctcatatag ggccctctca gggaacacaa catgaaggat aataggtata
    10381 acagtatctc ttctaatctc gcccacgaca cggcttattt ctgaagccct gtgcggagct
    10441 aagcccaggg ggcttgtagg tccagattcg gaagttgcaa gatcgtccac gataatctct
    10501 atggcatcga ctccaacatc caactcctga atatcaaccc ctttgtcaag cagagctgat
    10561 acaggtaaag ctagagcata ggtgaaacga cgctgttcaa ctggtacaga ggctagcggg
    10621 aacgcacttt ctacgaaagg tattgtcccc ttgggtagta taagacttaa aaacttcaga
    10681 aagtgtcttt ccgcacgttt tagggtaaga tatggggcgg tgtgcctctg atcggggaca
    10741 gctggaggac tagtacttgc ggagtgggta tctaggtttt cctctgaaac gttgaaaaat
    10801 tcaaaacggg tgcacctcct aagaactgtt gaggtagcgt ccaaaagcgt gcccacctta
    10861 gacttatcat atcccttcag gtgtgcctcg atagccttcg catctctcag aacatagatg
    10921 acaggattac tcacactaaa gtcttgcagc attgcctgta catctctctc aagccaacta
    10981 cataccaaaa tagctctagt cctatgcaaa ttcaggatgt tttgtaagat ggcgatttgc
    11041 cttctgccat aatcaacagg accatgcgtt tttttatagc ttgaactact caatcccgtc
    11101 aaatggtgga attcagattc taagtctaca atttttcttt tcatcgctgt gcttgccata
    11161 attgcaagcg tgctcttccc agcccccctg atccccgcaa tgacaatgct agcatcaggg
    11221 tcgaaggacg cggtaattgg atgatgtggc aaggcacgcc tcggaggtga gttcggtgcg
    11281 gaccagcttc ctagcgggac tctatgcgcg ctggttgacc tgctattact cagggtcctt
    11341 ggaggtcttc tcgcatgggc tccaacgtca ttgctgttgc cggtgtcgtt atacagcatc
    11401 gccattgttg cgaagctacg cttcatacca gatgctacgg agctagagat gtggggtagt
    11461 ggtaaaggat ctctggcggc aacgtcgccg acgtgtctag ctggaatggt gttcatgatt
    11521 ttagcggccg cttattgata tagtgtttaa gcgaatgaca gaagattaat ttcttggtat
    11581 gttaggaaag aataaaggag aataagaata attagaacaa tgtaggatgg aaagaaagat
    11641 tatcaagcat gccgacttta tatacttgaa cggaggcaaa ggatgcaaaa ttttctcaca
    11701 tttctttctg ccgttatgtt ggaagtaaga ctcccattat cgcaatactg caacacgaat
    11761 atgcaaaatt tgctgagtta tcgcagatag ttgttgcaaa gatagcggcg taggtggccg
    11821 cgaaatggga aattccaaaa caaacggttt ttttactcct gagaaatact tgtacgggat
    11881 aatccagggc ctaccaccca cgcttcgagg attggctttt attttttttt ttttggtggc
    11941 gttttatttc tttcccgctt tctgggactt gtgcggagtt ttgagagggg cgcgcggcaa
    12001 aggattccca aaacggaaat cagacgccaa tagccagcac tcaaagcagt tctggaccca
    12061 ttccgatttt cccatttggt tcttgcgcgt gctgattccg acacgcgcgt ctataaatag
    12121 catgaagtat ccgcacaccg cagcgttagt gaggtgaggg tggcagcaag ctaattcccg
    12181 catctggaat ctgaactgcc ccttttggac taaccgtgtg gttcatgggt gcccggcgat
    12241 aagagcgacc tcatgctata cctgagaaag caacctgacc tacaggaaag agttactcaa
    12301 gaataagaat tttcgtttta aaacctaaga gtcactttaa aatttgtata cacttatttt
    12361 ttttataact tatttaataa taaaaatcat aaatcataag aaattcgctg atcattactc
    12421 gagtttgtat agttcatcca tgccatgtgt aatcccagca gctgttacaa actcaagaag
    12481 gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg tggacaggta
    12541 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
    12601 tgctagttga acgcttccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc
    12661 attcttttgt ttgtctgcca tgatgtatac attgtgtgag ttatagttgt attccaattt
    12721 gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac
    12781 aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgaa
    12841 aaatatagtt ctttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg
    12901 tttcatatga tctgggtatc ttgaaaagca ttgaacacca taagtgaaag tagtgacaag
    12961 tgttggccat ggaacaggta gttttccagt agtgcaaata aatttaaggg taagttttcc
    13021 gtatgttgca tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc
    13081 atctaattca acaagaattg ggacaactcc agtgaaaagt tcttctcctt tgtcgacgct
    13141 agccatgatt ttagcggccg ctattaattt agtgtgtgta tttgtgtttg tgtgtctata
    13201 gaagtatagt aatttatgct gcaaaggtcc taatgtataa ggaaagaata tttagagaaa
    13261 agaagaaaac aagagtttta tatacataca gagcacatgc atgccatatg atcatgtgtc
    13321 gtcgcacaca tatatatatc tagaaaggat aagcgattac cctaggataa gcgattaccc
    13381 tagaggataa gcgattaccc ttgaggataa ttgtttatcc taggataagc gattacccac
    13441 tagtttaatt tattcttaag ctggcccggc gataacccgg cgataaatat gatacacggt
    13501 ccaatggata aacatttttt atcaacacta tgatatataa atataatagt ttttcgtata
    13561 tatattcctt tttttggtca atttttgaaa ttttcgtaga aaagggagag acaaaacaca
    13621 ttatatcaat gaaaacgtac aaaaagtaga taaagtcagt gcttaaacac gtcttttcct
    13681 taaaaatact ttattatttt tattttattg agagggtggt ttaaaaatag aaatagagag
    13741 agaggtacat acataaacat acgcgcacaa aagcagagat gatcattact cgagtcatta
    13801 ttgttcatac gtgttaatgt cactgaaatt gcacccaggg acgaacccaa gattcgtcat
    13861 aaattgtggg tcaacatcta cagcatcagt atattcaata ctcgctaaat catctaccat
    13921 ggcatcataa tcaactgtac tgtaaccaaa atttgccata gggaccaggt tgctatgata
    13981 gggagggtga ttagtcgttg agtccacgta cggtgggggt ggacgcggag gcgcccctcc
    14041 cggagctaga gctactgacg cctcgttaag gtgtgttgct ggaggggggt aggtgctcag
    14101 gattccgtgg gtattcacgg aaccgtaatc gttggtttgg gttatagcgt caagagcagc
    14161 gtcagaattg taaggcgtcg gatttggaat cccattaaaa cgatcattaa cagggtttat
    14221 tgacgtactc aagggttgtt ccatgacgtg aggcatgatt gacgcagtgg cagtagccgt
    14281 gggcggcagc tctaattgac ggagaagcct tgtgatactt ttattgaccc tggaggtcgc
    14341 ccaccttgca tgcagtcttt ccgccgccgg tgaatccttc cctaccagta acgctgagcc
    14401 ttggccagcg tccctgacta atagattatg tagcgcgtct tcggaccttg ctacgttttc
    14461 aaacatccac gccaacgcca attccaaggt cctaatataa ccagtctgaa cgccacgctt
    14521 cttgggggaa gcctgatacg tgcaagacct gccctgactc acgcaaggaa aacatgcagg
    14581 ctgaataccg tcacattttt cacgagcggc tctgcattgg tcacacgcac gactgaccct
    14641 ttgtctttta gacctggcag gctccaccaa taaggtggta gtagtcggtc ccaaccctgg
    14701 cgtatcgata ttcgtcaagt cagcgaaacg tgcgttgtta gaggaagccg cggtgttagc
    14761 tacatgaccg tccgcattac cgtccgcgtg gccat
//
