LOCUS       sequence-340108-       10519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes OptoQ-AMP3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10519)
  AUTHORS   Lalwani MA, Zhao EM, Wegner SA, Avalos JL
  TITLE     The Neurospora crassa Inducible Q System Enables Simultaneous 
            Optogenetic Amplification and Inversion in Saccharomyces cerevisiae 
            for Bidirectional Control of Gene Expression.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):2060-2075. doi: 
            10.1021/acssynbio.1c00229. Epub 2021 Aug 4.
  PUBMED    34346207
REFERENCE   2  (bases 1 to 10519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00229"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "2060-2075"
COMMENT     Sequence Label: pMAL722
FEATURES             Location/Qualifiers
     source          1..10519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(62..93)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     terminator      complement(264..511)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     477..495
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(1400..1420)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(1450..1847)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(2510..2529)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2511..2529)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2536..2553)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2536..2552)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2545..2567)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2693..3148
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2780..2799)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2990..3011
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3504..3523)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3623..3645
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3683..3701)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3785..3889
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3890..3958,3959..4750)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4108..4127)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4921..5509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5410..5429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5663..5680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5797..5818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5833..5838,5839..5856,5857..5863)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5871..5887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5876..5898
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5895..5911
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5895..5911
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5932..5950
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6276..6463
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     terminator      complement(7926..8091)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     8012..8036
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8107..8814)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(8147..8167)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     8762..8790
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctcttgggtg gcatgatttt agcggccgcg gtggctttac caacagtacc ggattgccaa
       61 gctggaagtc gagcttccat tatataccct ctagtgtctc ttaagcatgg actaaaggct
      121 acctataacg catggactaa aggctaccta taacgcatgg actaaaggct acctataacg
      181 catggactaa aggctaccta taacgcatgg actaaaggct acctactcga agctggcgaa
      241 ctcacgtacc cggcgataag gccgcaaatt aaagccttcg agcgtcccaa aaccttctca
      301 agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa
      361 atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc
      421 tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt
      481 gaatgtaagc gtgacataac taattacatg atcattactc gaggtcgact taaatacctg
      541 cttctacggc aattctaact aaatctgcac tcgtttttaa gtttaatttt tccattacca
      601 aacctctgtg cattttgaca gttttttctg aaagacccag tctggcagcc acttctttat
      661 ttcttaggcc tgatgcaacc agagttgtga cttctaattg acgtggtgaa agagttttta
      721 acatctccgc agcacgttct ctacgggcca tgcccatgtt cggttgatcg tcatccactt
      781 ccacttggct accaaggaag tatagtaatt catcatcgtc atcatagatt ggtgccacca
      841 aaactgcatt acgaaaagga gtaccatcct ttttgtaatt caaaatctca accaacacgg
      901 gtttatgctc acgaacacct tgacggatct tatctgttag ccagggttca gtaccagatc
      961 cctgtaagaa cctacaatta cgcccaacac attcttcctc agaataccca gttagatcgg
     1021 taaaggcctg gttgatagca atcaaaggat tatcagccaa tctgggatca ctaacaacac
     1081 ttgctatggg tgaggcctcg atcaaatcca atacccattg ggctggaggt tggacttcaa
     1141 ctctagtatc atctgctccg aattcacctc catattcgtc gataccaagt gcatcagtaa
     1201 acatctgttc aaattcgaaa tctgccatgt ccaacgcgcc atacggagca gaatcatgtg
     1261 gtgtaaatcc ggggccggga gaatccccat cacctaacat gtccagatca aaatcatcaa
     1321 gagcatcggc atgtgccata gctacatcct caccgtctaa atgtaattcg tcaccaagtg
     1381 aaacgtccgt tggtggggct acttttctct tttttttcgg gctagccatg attttagcgg
     1441 ccgccaaaac ttagattaga ttgctatgct ttctttctaa tgagcaagaa gtaaaaaaag
     1501 ttgtaataga acaagaaaaa tgaaactgaa acttgagaaa ttgaagaccg tttattaact
     1561 taaatatcaa tgggaggtca tcgaaagaga aaaaaatcaa aaaaaaaatt ttcaagaaaa
     1621 agaaacgtga taaaaatttt tattgccttt ttcgacgaag aaaaagaaac gaggcggtct
     1681 cttttttctt ttccaaacct ttagtacggg taattaacga caccctagag gaagaaagag
     1741 gggaaattta gtatgctgtg cttgggtgtt ttgaagtggt acggcgatgc gcggagtccg
     1801 agaaaatctg gaagagtaaa aaaggagtag aaacattttg aagctatcag ctgtttcccg
     1861 ggagctggaa ctcccacctg caacatgcgg gatcctgagt aaataagcgg ccgccaccgc
     1921 ggtggagctc agatcttgac accgattatt taaagctgca gcatacgata tatatacatg
     1981 tgtatatatg tatacctatg aatgtcagta agtatgtata cgaacagtat gatactgaag
     2041 atgacaaggt aatgcatcat tctatacgtg tcattctgaa cgaggcgcgc tttccttttt
     2101 tctttttgct ttttcttttt ttttctcttg aactccagaa aaaaaatata aaagagatgg
     2161 aggaacggga aaaagttagt tgtggtgata ggtggcaagt ggtattccgt aagaacaaca
     2221 agaaaagcat ttcatattat ggctgaactg agcgaacaag tgcaaaattt aagcatcaac
     2281 gacaacaacg agaatggtta tgttcctcct cacttaagag gaaaaccaag aagtgccaga
     2341 aataacagta gcaactacaa taacaacaac ggcggctaca acggtggccg tggcggtggc
     2401 agcttcttta gcaacaaccg tcgtggtggt tacggcaacg gtggtttctt cggtggaaac
     2461 aacggtggca gcggccggcc atttaaatgt ttaaacggcc acgctggccc cctatagtga
     2521 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     2581 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     2641 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     2701 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2761 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2821 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2881 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     2941 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3001 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3061 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3121 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     3181 gcggtatttc acaccgcata gggtaataac tgatataatt aaattgaagc tctaatttgt
     3241 gagtttagta ttaggatctc tacccttggc gaaaagtcct ctgccaacaa tgatgatatc
     3301 tgatccacca cttacaactt cgtcgagggt tctgtactgc tgacccaata tgcgtatata
     3361 taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc
     3421 aaaaaaattt caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa
     3481 aaggtggtat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     3541 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3601 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3661 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3721 ataataatgg tttcttagac gtcacgcgcc gaggacgtca ggtggcactt ttcggggaaa
     3781 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3841 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3901 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3961 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4021 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4081 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4141 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4201 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4261 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4321 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4381 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4441 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4501 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4561 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4621 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4681 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4741 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4801 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4861 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4921 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4981 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5041 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5101 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5161 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5221 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5281 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5341 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5401 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5461 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5521 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5581 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5641 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     5701 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     5761 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     5821 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     5881 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg gaattaaccc
     5941 tcactaaagg gaacaaaagc tgggccggcc atttaaatgt ttaaacggcc acgctggccg
     6001 ctgcacggtc ctgttcccta gcatgtacgt gagcgtattt ccttttaaac cacgacgctt
     6061 tgtcttcatt caacgtttcc cattgttttt ttctactatt gctttgctgt gggaaaaact
     6121 tatcgaaaga tgacgacttt ttcttaattc tcgttttaag agcttggtga gcgctaggag
     6181 tcactgccag gtatcgtttg aacacggcat tagtcaggga agtcataaca cagtcctttc
     6241 ccgcaatttt ctttttctat tactcttggc ctcctctagt acactctata tttttttatg
     6301 cctcggtaat gattttcatt tttttttttc cacctagcgg atgactcttt ttttttctta
     6361 gcgattggca ttatcacata atgaattata cattatataa agtaatgtga tttcttcgaa
     6421 gaatatacta aaaaatgagc aggcaagata aacgaaggca aagaggctct atatgtatat
     6481 aaagctgcgg atgccttctg atttaatcta gttctagttt acttcaggtt tacttaaagt
     6541 tgagataatt gaagcaaaga gaagcatcga gcaaaggatg gcgtttgtta agagggttac
     6601 gcaggagacg aatatacagc tggcgctgga tcttgacggt gggtctgttt ctgtacggga
     6661 gagcatactg ggcaaggaat atgctagtgg tgatgggcag accatccatg tgcacactgg
     6721 agttgggttt ttggaccaca tgttgactgc gctggcgaag catggcgggt ggtctctgat
     6781 cctggagtgt ataggggact tgcacatcga tgaccaccac actgttgagg actgtgggat
     6841 tgcgctgggc caagcgttca aggaggcgct tggctccgtg cgtggtatca agaggttcgg
     6901 gcatgggttt gcaccactgg acgaggcgct gagccgcgct gtggttgact tctccaatag
     6961 gcctttcgcc gtggtggagc tgggcctgaa gcgagaacgc ataggccagc tatccacaga
     7021 gatgatcccg cacttcttgg agagtttcgc cactgaggcg cgtatcacca tgcatgtgga
     7081 ctgtctgcgg ggcaccaacg accaccaccg ctccgaatca gctttcaagg cgctcgccat
     7141 cgccatcaga gaggcaagaa cacctacggg tcgcgatgac gttccatcca ctaagggtgt
     7201 cctagcatag aacacagccc acagctacca ccatcaacaa tatttatata gataacgtac
     7261 acatagaaat cacacaaaca gagtatttat tcttaactac atgaactacc atcagaccgt
     7321 ctgggccact atataatgtg ctattcataa acgtgatcac tctacgtagc aggcgacccc
     7381 aggtgaaaat ttttcagcga gctgccagat tgtcaggtga aaaactgaaa aaaacttctg
     7441 ggcgatgagc ttgtggcggg aaattaagta tataaagcag ttagtttctt ctgcttcttg
     7501 tggttctggg tattcttgta atatttggaa taatagaaaa ccaagactac tgcctttctt
     7561 ttcatattat agaggaagaa atacgcacga acacgatata gaggtaaatt ttttgcaaaa
     7621 agataccaaa ctataaaaga aatagaagac taaactacaa aaaataaaga aagtaataca
     7681 tatatccaat atgactgagt tagcagagca agtttccatg cctgaagggc aacaaagacg
     7741 tggtggttac gttccacctc acatccgtaa cggtggtggc agaagaggcg attctgctcc
     7801 tcgtggtggt ttcaacaacg gtggcggccg tggtggtttc ttcagaaaca acggtgaccg
     7861 tggtggattc ggctttgggt acctccggat atgttggcgc gccttaattt attcgccggc
     7921 gataagagcg acctcatgct atacctgaga aagcaacctg acctacagga aagagttact
     7981 caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt atacacttat
     8041 tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg ctgatcatta
     8101 ctcgagtttg tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag
     8161 aaggaccatg tggtctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag
     8221 gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg
     8281 gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt taactttgat
     8341 tccattcttt tgtttgtctg ccatgatgta tacattgtgt gagttatagt tgtattccaa
     8401 tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact cgattctatt
     8461 aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt
     8521 gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga aaaagtcatg
     8581 ccgtttcata tgatctgggt atcttgaaaa gcattgaaca ccataagtga aagtagtgac
     8641 aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa gggtaagttt
     8701 tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc cattaacatc
     8761 accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttgtcgac
     8821 gctagccatg attttagcgg ccgctattaa tttagtgtgt gtatttgtgt ttgtgtgtct
     8881 atagaagtat agtaatttat gctgcaaagg tcctaatgta taaggaaaga atatttagag
     8941 aaaagaagaa aacaagagtt ttatatacat acagagcaca tgcatgccat atgatcatgt
     9001 gtcgtcgcac acatatatat atctagaaag gataagcgat taccctagga taagcgatta
     9061 ccctagagga taagcgatta cccttgagga taattgttta tcctaggata agcgattacc
     9121 cactagttta atttattctt aagctggccc ggcgataacc cggcgataaa tatgatacac
     9181 ggtccaatgg ataaacattt tttatcaaca ctatgatata taaatataat agtttttcgt
     9241 atatatattc ctttttttgg tcaatttttg aaattttcgt agaaaaggga gagacaaaac
     9301 acattatatc aatgaaaacg tacaaaaagt agataaagtc agtgcttaaa cacgtctttt
     9361 ccttaaaaat actttattat ttttatttta ttgagagggt ggtttaaaaa tagaaataga
     9421 gagagaggta catacataaa catacgcgca caaaagcaga gatgatcatt actcgagtca
     9481 ttattgttca tacgtgttaa tgtcactgaa attgcaccca gggacgaacc caagattcgt
     9541 cataaattgt gggtcaacat ctacagcatc agtatattca atactcgcta aatcatctac
     9601 catggcatca taatcaactg tactgtaacc aaaatttgcc atagggacca ggttgctatg
     9661 atagggaggg tgattagtcg ttgagtccac gtacggtggg ggtggacgcg gaggcgcccc
     9721 tcccggagct agagctactg acgcctcgtt aaggtgtgtt gctggagggg ggtaggtgct
     9781 caggattccg tgggtattca cggaaccgta atcgttggtt tgggttatag cgtcaagagc
     9841 agcgtcagaa ttgtaaggcg tcggatttgg aatcccatta aaacgatcat taacagggtt
     9901 tattgacgta ctcaagggtt gttccatgac gtgaggcatg attgacgcag tggcagtagc
     9961 cgtgggcggc agctctaatt gacggagaag ccttgtgata cttttattga ccctggaggt
    10021 cgcccacctt gcatgcagtc tttccgccgc cggtgaatcc ttccctacca gtaacgctga
    10081 gccttggcca gcgtccctga ctaatagatt atgtagcgcg tcttcggacc ttgctacgtt
    10141 ttcaaacatc cacgccaacg ccaattccaa ggtcctaata taaccagtct gaacgccacg
    10201 cttcttgggg gaagcctgat acgtgcaaga cctgccctga ctcacgcaag gaaaacatgc
    10261 aggctgaata ccgtcacatt tttcacgagc ggctctgcat tggtcacacg cacgactgac
    10321 cctttgtctt ttagacctgg caggctccac caataaggtg gtagtagtcg gtcccaaccc
    10381 tggcgtatcg atattcgtca agtcagcgaa acgtgcgttg ttagaggaag ccgcggtgtt
    10441 agctacatga ccgtccgcat taccgtccgc gtggccatct gcatgggcgt tagcttccgc
    10501 tgccgcgttc agcgtcttt
//
