LOCUS       sequence-340110-       10519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes OptoQ-AMP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10519)
  AUTHORS   Lalwani MA, Zhao EM, Wegner SA, Avalos JL
  TITLE     The Neurospora crassa Inducible Q System Enables Simultaneous 
            Optogenetic Amplification and Inversion in Saccharomyces cerevisiae 
            for Bidirectional Control of Gene Expression.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):2060-2075. doi: 
            10.1021/acssynbio.1c00229. Epub 2021 Aug 4.
  PUBMED    34346207
REFERENCE   2  (bases 1 to 10519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00229"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "2060-2075"
COMMENT     Sequence Label: pMAL358
FEATURES             Location/Qualifiers
     source          1..10519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(30..61)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     terminator      complement(232..479)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     445..463
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(1368..1388)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(1418..1815)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(2478..2497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2479..2497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2504..2521)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2504..2520)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2513..2535)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2661..3116
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2748..2767)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2958..2979
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3472..3491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3591..3613
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3651..3669)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3753..3857
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3858..3926,3927..4718)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4076..4095)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4889..5477
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5378..5397
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5631..5648
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5765..5786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5801..5806,5807..5824,5825..5831)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5839..5855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5844..5866
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5863..5879
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5863..5879
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5900..5918
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6244..6431
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     terminator      complement(7894..8059)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     7980..8004
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8075..8782)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(8115..8135)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     8730..8758
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggctttacca acagtaccgg attgccaagc tggaagtcga gcttccatta tataccctct
       61 agtgtctctt aagcatggac taaaggctac ctataacgca tggactaaag gctacctata
      121 acgcatggac taaaggctac ctataacgca tggactaaag gctacctata acgcatggac
      181 taaaggctac ctactcgaag ctggcgaact cacgtacccg gcgataaggc cgcaaattaa
      241 agccttcgag cgtcccaaaa ccttctcaag caaggttttc agtataatgt tacatgcgta
      301 cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata aataacgttc ttaatactaa
      361 cataactata aaaaaataaa tagggaccta gacttcaggt tgtctaactc cttccttttc
      421 ggttagagcg gatgtggggg gagggcgtga atgtaagcgt gacataacta attacatgat
      481 cattactcga ggtcgactta aatacctgct tctacggcaa ttctaactaa atctgcactc
      541 gtttttaagt ttaatttttc cattaccaaa cctctgtgca ttttgacagt tttttctgaa
      601 agacccagtc tggcagccac ttctttattt cttaggcctg atgcaaccag agttgtgact
      661 tctaattgac gtggtgaaag agtttttaac atctccgcag cacgttctct acgggccatg
      721 cccatgttcg gttgatcgtc atccacttcc acttggctac caaggaagta tagtaattca
      781 tcatcgtcat catagattgg tgccaccaaa actgcattac gaaaaggagt accatccttt
      841 ttgtaattca aaatctcaac caacacgggt ttatgctcac gaacaccttg acggatctta
      901 tctgttagcc agggttcagt accagatcct gccaaaaatc tacaattacg accaacacat
      961 tcttcctcag aatacccagt tagatcggta aaggcctggt tgatagcaat caaaggatta
     1021 tcagccaatc tgggatcact aacaacactt gctatgggtg aggcctcgat caaatccaat
     1081 acccattggg ctggaggttg gacttcaact ctagtatcat ctgctccgaa ttcacctcca
     1141 tattcgtcga taccaagtgc atcagtaaac atctgttcaa attcgaaatc tgccatgtcc
     1201 aacgcgccat acggagcaga atcatgtggt gtaaatccgg ggccgggaga atccccatca
     1261 cctaacatgt ccagatcaaa atcatcaaga gcatcggcat gtgccatagc tacatcctca
     1321 ccgtctaaat gtaattcgtc accaagtgaa acgtccgttg gtggggctac ttttctcttt
     1381 tttttcgggc tagccatgat tttagcggcc gccaaaactt agattagatt gctatgcttt
     1441 ctttctaatg agcaagaagt aaaaaaagtt gtaatagaac aagaaaaatg aaactgaaac
     1501 ttgagaaatt gaagaccgtt tattaactta aatatcaatg ggaggtcatc gaaagagaaa
     1561 aaaatcaaaa aaaaaatttt caagaaaaag aaacgtgata aaaattttta ttgccttttt
     1621 cgacgaagaa aaagaaacga ggcggtctct tttttctttt ccaaaccttt agtacgggta
     1681 attaacgaca ccctagagga agaaagaggg gaaatttagt atgctgtgct tgggtgtttt
     1741 gaagtggtac ggcgatgcgc ggagtccgag aaaatctgga agagtaaaaa aggagtagaa
     1801 acattttgaa gctatcagct gtttcccggg agctggaact cccacctgca acatgcggga
     1861 tcctgagtaa ataagcggcc gccaccgcgg tggagctcag atcttgacac cgattattta
     1921 aagctgcagc atacgatata tatacatgtg tatatatgta tacctatgaa tgtcagtaag
     1981 tatgtatacg aacagtatga tactgaagat gacaaggtaa tgcatcattc tatacgtgtc
     2041 attctgaacg aggcgcgctt tccttttttc tttttgcttt ttcttttttt ttctcttgaa
     2101 ctccagaaaa aaaatataaa agagatggag gaacgggaaa aagttagttg tggtgatagg
     2161 tggcaagtgg tattccgtaa gaacaacaag aaaagcattt catattatgg ctgaactgag
     2221 cgaacaagtg caaaatttaa gcatcaacga caacaacgag aatggttatg ttcctcctca
     2281 cttaagagga aaaccaagaa gtgccagaaa taacagtagc aactacaata acaacaacgg
     2341 cggctacaac ggtggccgtg gcggtggcag cttctttagc aacaaccgtc gtggtggtta
     2401 cggcaacggt ggtttcttcg gtggaaacaa cggtggcagc ggccggccat ttaaatgttt
     2461 aaacggccac gctggccccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     2521 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2581 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2641 gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2701 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2761 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2821 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     2881 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2941 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3001 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3061 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg
     3121 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg gtaataactg
     3181 atataattaa attgaagctc taatttgtga gtttagtatt aggatctcta cccttggcga
     3241 aaagtcctct gccaacaatg atgatatctg atccaccact tacaacttcg tcgagggttc
     3301 tgtactgctg acccaatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt
     3361 cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa
     3421 aagaataaaa aaaaaatgat gaattgaaaa ggtggtatgg tgcactctca gtacaatctg
     3481 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     3541 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     3601 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     3661 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt cacgcgccga
     3721 ggacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3781 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3841 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3901 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3961 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4021 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4081 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4141 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4201 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4261 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4321 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4381 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4441 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4501 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4561 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4621 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4681 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4741 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4801 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4861 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4921 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4981 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5041 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5101 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5161 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5221 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5281 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5341 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5401 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5521 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5581 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5641 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5701 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     5761 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     5821 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     5881 atgattacgc caagctcgga attaaccctc actaaaggga acaaaagctg ggccggccat
     5941 ttaaatgttt aaacggccac gctggccgct gcacggtcct gttccctagc atgtacgtga
     6001 gcgtatttcc ttttaaacca cgacgctttg tcttcattca acgtttccca ttgttttttt
     6061 ctactattgc tttgctgtgg gaaaaactta tcgaaagatg acgacttttt cttaattctc
     6121 gttttaagag cttggtgagc gctaggagtc actgccaggt atcgtttgaa cacggcatta
     6181 gtcagggaag tcataacaca gtcctttccc gcaattttct ttttctatta ctcttggcct
     6241 cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt tttttttcca
     6301 cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat gaattataca
     6361 ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag gcaagataaa
     6421 cgaaggcaaa gaggctctat atgtatataa agctgcggat gccttctgat ttaatctagt
     6481 tctagtttac ttcaggttta cttaaagttg agataattga agcaaagaga agcatcgagc
     6541 aaaggatggc gtttgttaag agggttacgc aggagacgaa tatacagctg gcgctggatc
     6601 ttgacggtgg gtctgtttct gtacgggaga gcatactggg caaggaatat gctagtggtg
     6661 atgggcagac catccatgtg cacactggag ttgggttttt ggaccacatg ttgactgcgc
     6721 tggcgaagca tggcgggtgg tctctgatcc tggagtgtat aggggacttg cacatcgatg
     6781 accaccacac tgttgaggac tgtgggattg cgctgggcca agcgttcaag gaggcgcttg
     6841 gctccgtgcg tggtatcaag aggttcgggc atgggtttgc accactggac gaggcgctga
     6901 gccgcgctgt ggttgacttc tccaataggc ctttcgccgt ggtggagctg ggcctgaagc
     6961 gagaacgcat aggccagcta tccacagaga tgatcccgca cttcttggag agtttcgcca
     7021 ctgaggcgcg tatcaccatg catgtggact gtctgcgggg caccaacgac caccaccgct
     7081 ccgaatcagc tttcaaggcg ctcgccatcg ccatcagaga ggcaagaaca cctacgggtc
     7141 gcgatgacgt tccatccact aagggtgtcc tagcatagaa cacagcccac agctaccacc
     7201 atcaacaata tttatataga taacgtacac atagaaatca cacaaacaga gtatttattc
     7261 ttaactacat gaactaccat cagaccgtct gggccactat ataatgtgct attcataaac
     7321 gtgatcactc tacgtagcag gcgaccccag gtgaaaattt ttcagcgagc tgccagattg
     7381 tcaggtgaaa aactgaaaaa aacttctggg cgatgagctt gtggcgggaa attaagtata
     7441 taaagcagtt agtttcttct gcttcttgtg gttctgggta ttcttgtaat atttggaata
     7501 atagaaaacc aagactactg cctttctttt catattatag aggaagaaat acgcacgaac
     7561 acgatataga ggtaaatttt ttgcaaaaag ataccaaact ataaaagaaa tagaagacta
     7621 aactacaaaa aataaagaaa gtaatacata tatccaatat gactgagtta gcagagcaag
     7681 tttccatgcc tgaagggcaa caaagacgtg gtggttacgt tccacctcac atccgtaacg
     7741 gtggtggcag aagaggcgat tctgctcctc gtggtggttt caacaacggt ggcggccgtg
     7801 gtggtttctt cagaaacaac ggtgaccgtg gtggattcgg ctttgggtac ctccggatat
     7861 gttggcgcgc cttaatttat tcgccggcga taagagcgac ctcatgctat acctgagaaa
     7921 gcaacctgac ctacaggaaa gagttactca agaataagaa ttttcgtttt aaaacctaag
     7981 agtcacttta aaatttgtat acacttattt tttttataac ttatttaata ataaaaatca
     8041 taaatcataa gaaattcgct gatcattact cgagtttgta tagttcatcc atgccatgtg
     8101 taatcccagc agctgttaca aactcaagaa ggaccatgtg gtctctcttt tcgttgggat
     8161 ctttcgaaag ggcagattgt gtggacaggt aatggttgtc tggtaaaagg acagggccat
     8221 cgccaattgg agtattttgt tgataatggt ctgctagttg aacgcttcca tcttcaatgt
     8281 tgtgtctaat tttgaagtta actttgattc cattcttttg tttgtctgcc atgatgtata
     8341 cattgtgtga gttatagttg tattccaatt tgtgtccaag aatgtttcca tcttctttaa
     8401 aatcaatacc ttttaactcg attctattaa caagggtatc accttcaaac ttgacttcag
     8461 cacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt acataacctt
     8521 cgggcatggc actcttgaaa aagtcatgcc gtttcatatg atctgggtat cttgaaaagc
     8581 attgaacacc ataagtgaaa gtagtgacaa gtgttggcca tggaacaggt agttttccag
     8641 tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca ccctctccac
     8701 tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt gggacaactc
     8761 cagtgaaaag ttcttctcct ttgtcgacgc tagccatgat tttagcggcc gctattaatt
     8821 tagtgtgtgt atttgtgttt gtgtgtctat agaagtatag taatttatgc tgcaaaggtc
     8881 ctaatgtata aggaaagaat atttagagaa aagaagaaaa caagagtttt atatacatac
     8941 agagcacatg catgccatat gatcatgtgt cgtcgcacac atatatatat ctagaaagga
     9001 taagcgatta ccctaggata agcgattacc ctagaggata agcgattacc cttgaggata
     9061 attgtttatc ctaggataag cgattaccca ctagtttaat ttattcttaa gctggcccgg
     9121 cgataacccg gcgataaata tgatacacgg tccaatggat aaacattttt tatcaacact
     9181 atgatatata aatataatag tttttcgtat atatattcct ttttttggtc aatttttgaa
     9241 attttcgtag aaaagggaga gacaaaacac attatatcaa tgaaaacgta caaaaagtag
     9301 ataaagtcag tgcttaaaca cgtcttttcc ttaaaaatac tttattattt ttattttatt
     9361 gagagggtgg tttaaaaata gaaatagaga gagaggtaca tacataaaca tacgcgcaca
     9421 aaagcagaga tgatcattac tcgagtcatt attgttcata cgtgttaatg tcactgaaat
     9481 tgcacccagg gacgaaccca agattcgtca taaattgtgg gtcaacatct acagcatcag
     9541 tatattcaat actcgctaaa tcatctacca tggcatcata atcaactgta ctgtaaccaa
     9601 aatttgccat agggaccagg ttgctatgat agggagggtg attagtcgtt gagtccacgt
     9661 acggtggggg tggacgcgga ggcgcccctc ccggagctag agctactgac gcctcgttaa
     9721 ggtgtgttgc tggagggggg taggtgctca ggattccgtg ggtattcacg gaaccgtaat
     9781 cgttggtttg ggttatagcg tcaagagcag cgtcagaatt gtaaggcgtc ggatttggaa
     9841 tcccattaaa acgatcatta acagggttta ttgacgtact caagggttgt tccatgacgt
     9901 gaggcatgat tgacgcagtg gcagtagccg tgggcggcag ctctaattga cggagaagcc
     9961 ttgtgatact tttattgacc ctggaggtcg cccaccttgc atgcagtctt tccgccgccg
    10021 gtgaatcctt ccctaccagt aacgctgagc cttggccagc gtccctgact aatagattat
    10081 gtagcgcgtc ttcggacctt gctacgtttt caaacatcca cgccaacgcc aattccaagg
    10141 tcctaatata accagtctga acgccacgct tcttggggga agcctgatac gtgcaagacc
    10201 tgccctgact cacgcaagga aaacatgcag gctgaatacc gtcacatttt tcacgagcgg
    10261 ctctgcattg gtcacacgca cgactgaccc tttgtctttt agacctggca ggctccacca
    10321 ataaggtggt agtagtcggt cccaaccctg gcgtatcgat attcgtcaag tcagcgaaac
    10381 gtgcgttgtt agaggaagcc gcggtgttag ctacatgacc gtccgcatta ccgtccgcgt
    10441 ggccatctgc atgggcgtta gcttccgctg ccgcgttcag cgtctttctc ttgggtggca
    10501 tgattttagc ggccgcggt
//
