LOCUS       sequence-340145-        4589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pQE30 encoding Wzz mutant  i279.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Papadopoulos M, Morona R
  TITLE     Mutagenesis and chemical cross-linking suggest that Wzz dimer 
            stability and oligomerization affect lipopolysaccharide O-antigen 
            modal chain length control.
  JOURNAL   J Bacteriol. 2010 Jul;192(13):3385-93. doi: 10.1128/JB.01134-09. 
            Epub 2010 May 7.
  PUBMED    20453100
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01134-09"; journalName: "J Bacteriol"; date: "2010-07"; 
            volume: "192"; issue: "13"; pages: "3385-93"
COMMENT     Sequence Label: pMPRMA46
FEATURES             Location/Qualifiers
     source          1..4589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    60..76
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             95..106
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             125..142
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1366..1460
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1504..2163
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1570..1589)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2228..2314
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2361..2383)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2469..2609
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2524..2543
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2624..2641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2795..3383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2875..2894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3554..4345,4346..4414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4177..4196
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4415..4519)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(4587..4589,1..16)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aggcgtatca cgaggccctt tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt
       61 tgtgagcgga taacaatttc acacagaatt cattaaagag gagaaattaa ctatgagagg
      121 atcgcatcac catcaccatc acggatccgc atgcgagctc agagtagaaa ataataatgt
      181 ttctgggcaa aaccatgacc cggaacagat tgatttgatt gatttactag tgcagttgtg
      241 gcgtggcaag atgacaatta tcatttccgt cattgtggct attgccctgg ctattggtta
      301 tttggcagta gcgaaggaga aatggacgtc aacagcaatt atcactcagc ccgacgtggg
      361 gcaaattgct ggctataaca atgccatgaa tgttatctat ggtcaggctg caccgaaagt
      421 atcggatttg caggagacgt taattggtcg cttcagttct gccttctctg cattagcaga
      481 aacgctggat aatcaggaag agccagaaaa acttaccatc gaaccttctg ttaagaacca
      541 gcaattacca ttgactgttt cttatgttgg gcaaactgca gagggcgcac aaatgaagtt
      601 ggcccaatac attcagcaag ttgatgataa agtgaatcaa gagctagaaa aggatctcaa
      661 ggacaacatt gctctgggac ggaaaaactt gcaggactct ttaagaaccc aggaagtggt
      721 cgcgcaggag cagaaagatc tgcgtatccg tcagattcag gaagcgttgc agtatgcgaa
      781 tcaggcgcag gtgacaaagc cacaggttca gcagactgaa gatgtgacgc aagatacgtt
      841 gttccttcta gggagcgaag cgctggagtc gatgattaag catgaagcga ctcgtccgtt
      901 ggtgttctca ccaaactact atcagacacg tcaaaacctg ttggatattg aaaaattaaa
      961 gtttgatgat cttgatattc atgcttaccg ctattgcggc cgcagctatg tgatgaaacc
     1021 gacgttacct attcgtcgcg atagtccgaa aaaggcaatc accttgattc tggcagtgct
     1081 tctgggcggc atggttggcg cggggattgt gttggggcgt aacgctctgc gtaattacaa
     1141 cgcgaagtaa tattattgtg catttaagag aaacgggcag ggtggtgaca ccatgcccgt
     1201 tttttttgcc ggatgcgatg ctggcgcatc ttatccggcc tacgtgtgtt gagataatgt
     1261 gtaggcacga taagtttgcg catcgggcaa tggctccggg tgtgacaaca acatcacacc
     1321 tgctccccgg gtcgacctgc agccaagctt aattagctga gcttggactc ctgttgatag
     1381 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     1441 cgttttttat tggtgagaat ccaagctagc ttggcgagat tttcaggagc taaggaagct
     1501 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     1561 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     1621 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     1681 attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac
     1741 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     1801 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     1861 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     1921 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     1981 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     2041 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     2101 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     2161 taattttttt aaggcagtta ttggtgccct taaacgcctg gggtaatgac tctctagctt
     2221 gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2281 tttgtcggtg aacgctctcc tgagtaggac aaatccgccc tctagagctg cctcgcgcgt
     2341 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     2401 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     2461 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     2521 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     2581 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     2641 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2701 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2761 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2821 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2881 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2941 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3001 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3061 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3121 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3181 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3241 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3301 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3361 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3421 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3481 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3541 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3601 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3661 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3721 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3781 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3841 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3901 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3961 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4021 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4081 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4141 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4201 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4261 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4321 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4381 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4441 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4501 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     4561 ttattatcat gacattaacc tataaaaat
//
