LOCUS       sequence-340146-        4589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pQE30 encoding Wzz mutant  i66.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Papadopoulos M, Morona R
  TITLE     Mutagenesis and chemical cross-linking suggest that Wzz dimer 
            stability and oligomerization affect lipopolysaccharide O-antigen 
            modal chain length control.
  JOURNAL   J Bacteriol. 2010 Jul;192(13):3385-93. doi: 10.1128/JB.01134-09. 
            Epub 2010 May 7.
  PUBMED    20453100
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01134-09"; journalName: "J Bacteriol"; date: "2010-07"; 
            volume: "192"; issue: "13"; pages: "3385-93"
COMMENT     Sequence Label: pMPRMA56
FEATURES             Location/Qualifiers
     source          1..4589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(199..990,991..1059))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     822..841
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1060..1164)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1232..1250
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    1294..1310
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1329..1340
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1359..1376
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2600..2694
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             2738..3397
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2804..2823)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3462..3548
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3595..3617)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3703..3843
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3758..3777
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3858..3875)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(4029..4589,1..28))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4109..4128)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
       61 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
      121 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
      181 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
      241 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
      301 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
      361 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
      421 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
      481 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
      541 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
      601 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
      661 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
      721 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
      781 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
      841 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
      901 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
      961 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     1021 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     1081 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     1141 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     1201 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     1261 tcacctcgag aaatcataaa aaatttattt gctttgtgag cggataacaa tttcacacag
     1321 aattcattaa agaggagaaa ttaactatga gaggatcgca tcaccatcac catcacggat
     1381 ccgcatgcga gctcagagta gaaaataata atgtttctgg gcaaaaccat gacccggaac
     1441 agattgattt gattgattta ctagtgcagt tgtggcgtgg caagatgaca attatcattt
     1501 ccgtcattgt ggctattgcc ctggctattg gttatttggc agtagcgaag gagaaatgga
     1561 cgtcaacagc aattatcact cagcccgact gcggccgcac cgacgtgggg caaattgctg
     1621 gctataacaa tgccatgaat gttatctatg gtcaggctgc accgaaagta tcggatttgc
     1681 aggagacgtt aattggtcgc ttcagttctg ccttctctgc attagcagaa acgctggata
     1741 atcaggaaga gccagaaaaa cttaccatcg aaccttctgt taagaaccag caattaccat
     1801 tgactgtttc ttatgttggg caaactgcag agggcgcaca aatgaagttg gcccaataca
     1861 ttcagcaagt tgatgataaa gtgaatcaag agctagaaaa ggatctcaag gacaacattg
     1921 ctctgggacg gaaaaacttg caggactctt taagaaccca ggaagtggtc gcgcaggagc
     1981 agaaagatct gcgtatccgt cagattcagg aagcgttgca gtatgcgaat caggcgcagg
     2041 tgacaaagcc acaggttcag cagactgaag atgtgacgca agatacgttg ttccttctag
     2101 ggagcgaagc gctggagtcg atgattaagc atgaagcgac tcgtccgttg gtgttctcac
     2161 caaactacta tcagacacgt caaaacctgt tggatattga aaaattaaag tttgatgatc
     2221 ttgatattca tgcttaccgc tatgtgatga aaccgacgtt acctattcgt cgcgatagtc
     2281 cgaaaaaggc aatcaccttg attctggcag tgcttctggg cggcatggtt ggcgcgggga
     2341 ttgtgttggg gcgtaacgct ctgcgtaatt acaacgcgaa gtaatattat tgtgcattta
     2401 agagaaacgg gcagggtggt gacaccatgc ccgttttttt tgccggatgc gatgctggcg
     2461 catcttatcc ggcctacgtg tgttgagata atgtgtaggc acgataagtt tgcgcatcgg
     2521 gcaatggctc cgggtgtgac aacaacatca cacctgctcc ccgggtcgac ctgcagccaa
     2581 gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc agaactccat
     2641 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc
     2701 tagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     2761 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     2821 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     2881 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     2941 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     3001 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     3061 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     3121 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     3181 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     3241 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     3301 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     3361 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg
     3421 cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa cgaaaggctc
     3481 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     3541 ggacaaatcc gccctctaga gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     3601 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     3661 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     3721 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     3781 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     3841 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3901 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3961 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4021 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4201 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4441 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     4501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4561 agcggtggtt tttttgtttg caagcagca
//
