LOCUS       sequence-34015-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Taylor MJ, Perrais D, Merrifield CJ
  TITLE     A high precision survey of the molecular dynamics of Mammalian 
            clathrin-mediated endocytosis.
  JOURNAL   PLoS Biol. 2011 Mar . 9(3):e1000604.
  PUBMED    21445324
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2011 Mar . 9(3):e1000604."
COMMENT     Sequence Label: AP2u2-mCherry
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      322..331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg
       61 taccgagctc ggatcgatat ctgcggccta gctagcacca tgatcggagg cttattcatc
      121 tataatcaca agggggaggt gcttatctcc cgagtctaca gagatgacat cgggaggaat
      181 gcagtggatg cctttcgcgt caatgtcatt cacgcacggc agcaggtgcg cagccctgtc
      241 acaaacatcg ctcgcaccag cttcttccat gttaagcggt ccaacatctg gctggcagca
      301 gtcaccaagc agaatgtcaa tgccgccatg gtcttcgaat tcctctacaa gatgtgtgat
      361 gtaatggctg cttactttgg caagatcagc gaggagaaca tcaagaacaa tttcgtgctc
      421 atatacgagc tgctggacga gattctggac tttggctacc cacagaactc agagacaggt
      481 gcactgaaaa ccttcatcac ccaacagggt atcaagagtc agcatcagac aaaagaagag
      541 cagtcccaga tcaccagcca ggtgaccggg caaattggct ggaggcggga aggcatcaag
      601 tatcgtcgga atgaactctt cctagatgtt ctggagagtg tgaacctgct tatgtcccca
      661 caggggcagg tgctgagtgc ccatgtgtcg ggccgggtgg tgatgaagag ttacctgagc
      721 ggcatgcctg agtgcaagtt tggaatgaat gacaagattg tcatagaaaa gcagggcaaa
      781 ggcacagccg atgaaacaag caagagtaga tctatggtga gcaagggcga gga
//
