LOCUS       sequence-340152-       14784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes OptoQ-AMP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14784)
  AUTHORS   Lalwani MA, Zhao EM, Wegner SA, Avalos JL
  TITLE     The Neurospora crassa Inducible Q System Enables Simultaneous 
            Optogenetic Amplification and Inversion in Saccharomyces cerevisiae 
            for Bidirectional Control of Gene Expression.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):2060-2075. doi: 
            10.1021/acssynbio.1c00229. Epub 2021 Aug 4.
  PUBMED    34346207
REFERENCE   2  (bases 1 to 14784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00229"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "2060-2075"
COMMENT     Sequence Label: pMAL716
FEATURES             Location/Qualifiers
     source          1..14784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(90..121)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     terminator      complement(292..539)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     505..523
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(1428..1448)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(1478..1875)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(2538..2557)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2539..2557)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2564..2581)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2564..2580)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2573..2595)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2721..3176
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2808..2827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3018..3039
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3532..3551)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3651..3673
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3711..3729)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3813..3917
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3918..3986,3987..4778)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4136..4155)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4949..5537
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5438..5457
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5691..5708
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5825..5846
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5861..5866,5867..5884,5885..5891)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5899..5915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5904..5926
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5923..5939
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5923..5939
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5960..5978
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6304..6491
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     terminator      complement(12230..12395)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     12316..12340
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(12411..13118)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(12451..12471)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     13066..13094
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcttccgct gccgcgttca gcgtctttct cttgggtggc atgattttag cggccgcggt
       61 ggctttacca acagtaccgg attgccaagc tggaagtcga gcttccatta tataccctct
      121 agtgtctctt aagcatggac taaaggctac ctataacgca tggactaaag gctacctata
      181 acgcatggac taaaggctac ctataacgca tggactaaag gctacctata acgcatggac
      241 taaaggctac ctactcgaag ctggcgaact cacgtacccg gcgataaggc cgcaaattaa
      301 agccttcgag cgtcccaaaa ccttctcaag caaggttttc agtataatgt tacatgcgta
      361 cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata aataacgttc ttaatactaa
      421 cataactata aaaaaataaa tagggaccta gacttcaggt tgtctaactc cttccttttc
      481 ggttagagcg gatgtggggg gagggcgtga atgtaagcgt gacataacta attacatgat
      541 cattactcga ggtcgactta aatacctgct tctacggcaa ttctaactaa atctgcactc
      601 gtttttaagt ttaatttttc cattaccaaa cctctgtgca ttttgacagt tttttctgaa
      661 agacccagtc tggcagccac ttctttattt cttaggcctg atgcaaccag agttgtgact
      721 tctaattgac gtggtgaaag agtttttaac atctccgcag cacgttctct acgggccatg
      781 cccatgttcg gttgatcgtc atccacttcc acttggctac caaggaagta tagtaattca
      841 tcatcgtcat catagattgg tgccaccaaa actgcattac gaaaaggagt accatccttt
      901 ttgtaattca aaatctcaac caacacgggt ttatgctcac gaacaccttg acggatctta
      961 tctgttagcc agggttcagt accagatcct gccaaaaatc tacaattacg accaacacat
     1021 tcttcctcag aatacccagt tagatcggta aaggcctggt tgatagcaat caaaggatta
     1081 tcagccaatc tgggatcact aacaacactt gctatgggtg aggcctcgat caaatccaat
     1141 acccattggg ctggaggttg gacttcaact ctagtatcat ctgctccgaa ttcacctcca
     1201 tattcgtcga taccaagtgc atcagtaaac atctgttcaa attcgaaatc tgccatgtcc
     1261 aacgcgccat acggagcaga atcatgtggt gtaaatccgg ggccgggaga atccccatca
     1321 cctaacatgt ccagatcaaa atcatcaaga gcatcggcat gtgccatagc tacatcctca
     1381 ccgtctaaat gtaattcgtc accaagtgaa acgtccgttg gtggggctac ttttctcttt
     1441 tttttcgggc tagccatgat tttagcggcc gccaaaactt agattagatt gctatgcttt
     1501 ctttctaatg agcaagaagt aaaaaaagtt gtaatagaac aagaaaaatg aaactgaaac
     1561 ttgagaaatt gaagaccgtt tattaactta aatatcaatg ggaggtcatc gaaagagaaa
     1621 aaaatcaaaa aaaaaatttt caagaaaaag aaacgtgata aaaattttta ttgccttttt
     1681 cgacgaagaa aaagaaacga ggcggtctct tttttctttt ccaaaccttt agtacgggta
     1741 attaacgaca ccctagagga agaaagaggg gaaatttagt atgctgtgct tgggtgtttt
     1801 gaagtggtac ggcgatgcgc ggagtccgag aaaatctgga agagtaaaaa aggagtagaa
     1861 acattttgaa gctatcagct gtttcccggg agctggaact cccacctgca acatgcggga
     1921 tcctgagtaa ataagcggcc gccaccgcgg tggagctcag atcttgacac cgattattta
     1981 aagctgcagc atacgatata tatacatgtg tatatatgta tacctatgaa tgtcagtaag
     2041 tatgtatacg aacagtatga tactgaagat gacaaggtaa tgcatcattc tatacgtgtc
     2101 attctgaacg aggcgcgctt tccttttttc tttttgcttt ttcttttttt ttctcttgaa
     2161 ctccagaaaa aaaatataaa agagatggag gaacgggaaa aagttagttg tggtgatagg
     2221 tggcaagtgg tattccgtaa gaacaacaag aaaagcattt catattatgg ctgaactgag
     2281 cgaacaagtg caaaatttaa gcatcaacga caacaacgag aatggttatg ttcctcctca
     2341 cttaagagga aaaccaagaa gtgccagaaa taacagtagc aactacaata acaacaacgg
     2401 cggctacaac ggtggccgtg gcggtggcag cttctttagc aacaaccgtc gtggtggtta
     2461 cggcaacggt ggtttcttcg gtggaaacaa cggtggcagc ggccggccat ttaaatgttt
     2521 aaacggccac gctggccccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     2581 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     2641 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     2701 gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2761 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2821 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2881 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     2941 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3001 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3061 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3121 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg
     3181 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg gtaataactg
     3241 atataattaa attgaagctc taatttgtga gtttagtatt aggatctcta cccttggcga
     3301 aaagtcctct gccaacaatg atgatatctg atccaccact tacaacttcg tcgagggttc
     3361 tgtactgctg acccaatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt
     3421 cttccttctg ttcggagatt accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa
     3481 aagaataaaa aaaaaatgat gaattgaaaa ggtggtatgg tgcactctca gtacaatctg
     3541 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     3601 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     3661 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     3721 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt cacgcgccga
     3781 ggacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3841 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3901 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3961 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4021 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4081 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4141 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4201 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4261 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4321 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4381 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4441 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4501 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4561 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4621 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4681 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4741 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4801 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4861 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4921 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4981 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5041 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5101 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5161 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5221 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5281 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5341 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5401 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5461 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5521 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5581 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5641 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5701 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5761 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     5821 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     5881 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     5941 atgattacgc caagctcgga attaaccctc actaaaggga acaaaagctg ggccggccat
     6001 ttaaatgttt aaacggccac gctggccgct gcacggtcct gttccctagc atgtacgtga
     6061 gcgtatttcc ttttaaacca cgacgctttg tcttcattca acgtttccca ttgttttttt
     6121 ctactattgc tttgctgtgg gaaaaactta tcgaaagatg acgacttttt cttaattctc
     6181 gttttaagag cttggtgagc gctaggagtc actgccaggt atcgtttgaa cacggcatta
     6241 gtcagggaag tcataacaca gtcctttccc gcaattttct ttttctatta ctcttggcct
     6301 cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt tttttttcca
     6361 cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat gaattataca
     6421 ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag gcaagataaa
     6481 cgaaggcaaa gaggctctat atgtatataa agctgcggat gccttctgat ttaatctagt
     6541 tctagtttac ttcaggttta cttaaagttg agataattga agcaaagaga agcatcgagc
     6601 aaaggatggc gtttgttaag agggttacgc aggagacgaa tatacagctg gcgctggatc
     6661 ttgacggtgg gtctgtttct gtacgggaga gcatactggg caaggaatat gctagtggtg
     6721 atgggcagac catccatgtg cacactggag ttgggttttt ggaccacatg ttgactgcgc
     6781 tggcgaagca tggcgggtgg tctctgatcc tggagtgtat aggggacttg cacatcgatg
     6841 accaccacac tgttgaggac tgtgggattg cgctgggcca agcgttcaag gaggcgcttg
     6901 gctccgtgcg tggtatcaag aggttcgggc atgggtttgc accactggac gaggcgctga
     6961 gccgcgctgt ggttgacttc tccaataggc ctttcgccgt ggtggagctg ggcctgaagc
     7021 gagaacgcat aggccagcta tccacagaga tgatcccgca cttcttggag agtttcgcca
     7081 ctgaggcgcg tatcaccatg catgtggact gtctgcgggg caccaacgac caccaccgct
     7141 ccgaatcagc tttcaaggcg ctcgccatcg ccatcagaga ggcaagaaca cctacgggtc
     7201 gcgatgacgt tccatccact aagggtgtcc tagcatagaa cacagcccac agctaccacc
     7261 atcaacaata tttatataga taacgtacac atagaaatca cacaaacaga gtatttattc
     7321 ttaactacat gaactaccat cagaccgtct gggccactat ataatgtgct attcataaac
     7381 gtgatcactc tacgtagcag gcgaccccag gtgaaaattt ttcagcgagc tgccagattg
     7441 tcaggtgaaa aactgaaaaa aacttctggg cgatgagctt gtggcgggaa attaagtata
     7501 taaagcagtt agtttcttct gcttcttgtg gttctgggta ttcttgtaat atttggaata
     7561 atagaaaacc aagactactg cctttctttt catattatag aggaagaaat acgcacgaac
     7621 acgatataga ggtaaatttt ttgcaaaaag ataccaaact ataaaagaaa tagaagacta
     7681 aactacaaaa aataaagaaa gtaatacata tatccaatat gactgagtta gcagagcaag
     7741 tttccatgcc tgaagggcaa caaagacgtg gtggttacgt tccacctcac atccgtaacg
     7801 gtggtggcag aagaggcgat tctgctcctc gtggtggttt caacaacggt ggcggccgtg
     7861 gtggtttctt cagaaacaac ggtgaccgtg gtggattcgg ctttgggtac ctccggatat
     7921 gttggcgcgc cttaatttat tcgccggcga tacatgggtg accaaaagag cgggcggata
     7981 cacgcgtcac cacaagcaga ataaaaggta aacctgaaat tgttttaaca taaaatgaaa
     8041 aatgcttgtt tgcaacccta tatagaatca taaaacattc gtgactataa aatgaataaa
     8101 ctaaactatt ctaagaaaat gaaataaatg acaaaaaaac gtgttttttg gactagaagg
     8161 cttaatcaaa agctctcgat cattattatt ggaagtacaa gttttcagaa ccagccttct
     8221 tgtacaaaga agtaatagat tcttgtgcac aagacatagg aagttctcga accgcttcat
     8281 cgatattcac agctgttttt ttcaccaggt cttctccctc tttcactgcg gtttcttcaa
     8341 tgacattgcg aactggttct acgtgcttgc tcccgtctag ttgaactcca ataaagtatt
     8401 gtacttctcc cttctgatca cgcataggtt gcaagtggaa aatgttccag aacttctttc
     8461 cgctcttggt gtagttgatg agctgaactg tcacttcggt ttggttatca atagcatttc
     8521 gaatcttctt cactgtggtt agatcagtct ctggaccttg tagaaacctg caatttcttc
     8581 caagaatttc ttcacggcta tattccgtga gctccaagaa actatcagat gcaaaaataa
     8641 tgggattatc gggaagccta ggatcagtga taataaaatt cttctcgata cgttcgagtg
     8701 tagtagctag atcaataccc tttctcatct gtcctccagc actctcgaga gagatttgcg
     8761 tggctatccc gtttagacgg ggctgtagtt gtgccgtgtg acttttagcc tggtcgtctg
     8821 gatacgcacg tagcacttca cgcctcatca acctacgcgg cgcacgtcta cctgtaaaca
     8881 gttcgaactg ggcaaagcct tgttcaggca gtaagtcaag tccgtccatg gccacccagc
     8941 cacggtgagc ttcacgacgt gtctgctcta gcagtgggga ggttaagcac ttataatcta
     9001 actctagaac tataccgccg gtaggattgt caagccagtg tagggggact gtaaattccg
     9061 ggtccggtgt gttatcgact gtatgggttg gaatacagct cacgatcata gtaggatttt
     9121 tgaaattctc tggcaatggg tcatctctag aacggatgat atgaaagcac gtattatcat
     9181 gactcctggg ccccgtgctt aacaatggaa gtgcatcacg aactaacagg ttttcgaaat
     9241 gaagaaccag tttttcagca ttagcgaaag tacggttgaa aattaaaatt tttttgaccc
     9301 caagttgcag cattgcatac actgcggctc tcgccatccc cccggcacct atcactaaac
     9361 cggtgcttgt agacctcact gcgtttgctg ggctaagccc ccttcttaaa catgctctta
     9421 ttccaatcca atcagtattt tcaccataaa gagctctcac tgctcccgcc tgagaaatgt
     9481 tattaaacat actgacttca tctggaatgc ccccgtccgc agtaagatga cgcactggga
     9541 ttaacgtatt cacggctccg atagcctttg cgtgtctaga cagactatgt gtaagtgaaa
     9601 taatctcaac tttgaacggt aaccctactg aagctccagc aaattgtgga tcactaatta
     9661 cttccctcaa agtccctata ttagcagttg atagcggttt atagtggtgt ggtattccgc
     9721 aagcttttag cgctgcgttg tgcatagccg gtgaaaggga gtagccaacg ttagccccga
     9781 acacatataa tttcatagga tcgtgcacaa acgcggaata aagggcttgt gtggcttcca
     9841 gtgctgttag aggaggctgt aagtatctgt gagccggatt acgaaggcct atcgcatcct
     9901 ccttcgtcgg tactggggtg acgggggtca ggatctcatt aaagcacata gacgttcttc
     9961 ccaatctccc ggtgttataa gcgataaatg gcagcctagg tcccccaacc gcctcaactg
    10021 caacatggaa ctgtctaata tctgtgttat cacggggatt gctagcgggt ctggtcaacc
    10081 tcactaaatc acaccctgtg ttttgtgctt tctcgtatgc ttgtaaccaa ctcggatcac
    10141 cccatctcgg ggagtttacc tctgcaagtt gtttatttcc tatcactttc gtggtccctt
    10201 ggacggttgt tagctgtcct aacaaaccgc tgtcaagccc aaggtctacg gtcaggtagt
    10261 cgggtgcaag cctaagtgca tggttaagat aagtcatgta caacgctaga agggcttcct
    10321 catatagggc cctctcaggg aacacaacat gaaggataat aggtataaca gtatctcttc
    10381 taatctcgcc cacgacacgg cttatttctg aagccctgtg cggagctaag cccagggggc
    10441 ttgtaggtcc agattcggaa gttgcaagat cgtccacgat aatctctatg gcatcgactc
    10501 caacatccaa ctcctgaata tcaacccctt tgtcaagcag agctgataca ggtaaagcta
    10561 gagcataggt gaaacgacgc tgttcaactg gtacagaggc tagcgggaac gcactttcta
    10621 cgaaaggtat tgtccccttg ggtagtataa gacttaaaaa cttcagaaag tgtctttccg
    10681 cacgttttag ggtaagatat ggggcggtgt gcctctgatc ggggacagct ggaggactag
    10741 tacttgcgga gtgggtatct aggttttcct ctgaaacgtt gaaaaattca aaacgggtgc
    10801 acctcctaag aactgttgag gtagcgtcca aaagcgtgcc caccttagac ttatcatatc
    10861 ccttcaggtg tgcctcgata gccttcgcat ctctcagaac atagatgaca ggattactca
    10921 cactaaagtc ttgcagcatt gcctgtacat ctctctcaag ccaactacat accaaaatag
    10981 ctctagtcct atgcaaattc aggatgtttt gtaagatggc gatttgcctt ctgccataat
    11041 caacaggacc atgcgttttt ttatagcttg aactactcaa tcccgtcaaa tggtggaatt
    11101 cagattctaa gtctacaatt tttcttttca tcgctgtgct tgccataatt gcaagcgtgc
    11161 tcttcccagc ccccctgatc cccgcaatga caatgctagc atcagggtcg aaggacgcgg
    11221 taattggatg atgtggcaag gcacgcctcg gaggtgagtt cggtgcggac cagcttccta
    11281 gcgggactct atgcgcgctg gttgacctgc tattactcag ggtccttgga ggtcttctcg
    11341 catgggctcc aacgtcattg ctgttgccgg tgtcgttata cagcatcgcc attgttgcga
    11401 agctacgctt cataccagat gctacggagc tagagatgtg gggtagtggt aaaggatctc
    11461 tggcggcaac gtcgccgacg tgtctagctg gaatggtgtt catgatttta gcggccgctt
    11521 attgatatag tgtttaagcg aatgacagaa gattaatttc ttggtatgtt aggaaagaat
    11581 aaaggagaat aagaataatt agaacaatgt aggatggaaa gaaagattat caagcatgcc
    11641 gactttatat acttgaacgg aggcaaagga tgcaaaattt tctcacattt ctttctgccg
    11701 ttatgttgga agtaagactc ccattatcgc aatactgcaa cacgaatatg caaaatttgc
    11761 tgagttatcg cagatagttg ttgcaaagat agcggcgtag gtggccgcga aatgggaaat
    11821 tccaaaacaa acggtttttt tactcctgag aaatacttgt acgggataat ccagggccta
    11881 ccacccacgc ttcgaggatt ggcttttatt tttttttttt tggtggcgtt ttatttcttt
    11941 cccgctttct gggacttgtg cggagttttg agaggggcgc gcggcaaagg attcccaaaa
    12001 cggaaatcag acgccaatag ccagcactca aagcagttct ggacccattc cgattttccc
    12061 atttggttct tgcgcgtgct gattccgaca cgcgcgtcta taaatagcat gaagtatccg
    12121 cacaccgcag cgttagtgag gtgagggtgg cagcaagcta attcccgcat ctggaatctg
    12181 aactgcccct tttggactaa ccgtgtggtt catgggtgcc cggcgataag agcgacctca
    12241 tgctatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa taagaatttt
    12301 cgttttaaaa cctaagagtc actttaaaat ttgtatacac ttattttttt tataacttat
    12361 ttaataataa aaatcataaa tcataagaaa ttcgctgatc attactcgag tttgtatagt
    12421 tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac catgtggtct
    12481 ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt
    12541 aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg
    12601 cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt cttttgtttg
    12661 tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg
    12721 tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct
    12781 tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt
    12841 tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgccgttt catatgatct
    12901 gggtatcttg aaaagcattg aacaccataa gtgaaagtag tgacaagtgt tggccatgga
    12961 acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca
    13021 ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca
    13081 agaattggga caactccagt gaaaagttct tctcctttgt cgacgctagc catgatttta
    13141 gcggccgcta ttaatttagt gtgtgtattt gtgtttgtgt gtctatagaa gtatagtaat
    13201 ttatgctgca aaggtcctaa tgtataagga aagaatattt agagaaaaga agaaaacaag
    13261 agttttatat acatacagag cacatgcatg ccatatgatc atgtgtcgtc gcacacatat
    13321 atatatctag aaaggataag cgattacccg aggataagcg attacccgag cggataagcg
    13381 attacccgtg aggataattg tttatccgag gataagcgat tacccactag tttaatttat
    13441 tcttaagctg gcccggcgat aaatatgata cacggtccaa tggataaaca ttttttatca
    13501 acactatgat atataaatat aatagttttt cgtatatata ttcctttttt tggtcaattt
    13561 ttgaaatttt cgtagaaaag ggagagacaa aacacattat atcaatgaaa acgtacaaaa
    13621 agtagataaa gtcagtgctt aaacacgtct tttccttaaa aatactttat tatttttatt
    13681 ttattgagag ggtggtttaa aaatagaaat agagagagag gtacatacat aaacatacgc
    13741 gcacaaaagc agagatgatc attactcgag tcattattgt tcatacgtgt taatgtcact
    13801 gaaattgcac ccagggacga acccaagatt cgtcataaat tgtgggtcaa catctacagc
    13861 atcagtatat tcaatactcg ctaaatcatc taccatggca tcataatcaa ctgtactgta
    13921 accaaaattt gccataggga ccaggttgct atgataggga gggtgattag tcgttgagtc
    13981 cacgtacggt gggggtggac gcggaggcgc ccctcccgga gctagagcta ctgacgcctc
    14041 gttaaggtgt gttgctggag gggggtaggt gctcaggatt ccgtgggtat tcacggaacc
    14101 gtaatcgttg gtttgggtta tagcgtcaag agcagcgtca gaattgtaag gcgtcggatt
    14161 tggaatccca ttaaaacgat cattaacagg gtttattgac gtactcaagg gttgttccat
    14221 gacgtgaggc atgattgacg cagtggcagt agccgtgggc ggcagctcta attgacggag
    14281 aagccttgtg atacttttat tgaccctgga ggtcgcccac cttgcatgca gtctttccgc
    14341 cgccggtgaa tccttcccta ccagtaacgc tgagccttgg ccagcgtccc tgactaatag
    14401 attatgtagc gcgtcttcgg accttgctac gttttcaaac atccacgcca acgccaattc
    14461 caaggtccta atataaccag tctgaacgcc acgcttcttg ggggaagcct gatacgtgca
    14521 agacctgccc tgactcacgc aaggaaaaca tgcaggctga ataccgtcac atttttcacg
    14581 agcggctctg cattggtcac acgcacgact gaccctttgt cttttagacc tggcaggctc
    14641 caccaataag gtggtagtag tcggtcccaa ccctggcgta tcgatattcg tcaagtcagc
    14701 gaaacgtgcg ttgttagagg aagccgcggt gttagctaca tgaccgtccg cattaccgtc
    14761 cgcgtggcca tctgcatggg cgtt
//
