LOCUS       sequence-340154-       12464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes OptoAMP2 circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12464)
  AUTHORS   Zhao EM, Lalwani MA, Chen JM, Orillac P, Toettcher JE, Avalos JL
  TITLE     Optogenetic Amplification Circuits for Light-Induced Metabolic 
            Control.
  JOURNAL   ACS Synth Biol. 2021 May 21;10(5):1143-1154. doi: 
            10.1021/acssynbio.0c00642. Epub 2021 Apr 9.
  PUBMED    33835777
REFERENCE   2  (bases 1 to 12464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00642"; journalName: "ACS Synth Biol"; date: 
            "2021-05-21- 21"; volume: "10"; issue: "5"; pages: "1143-1154"
COMMENT     Sequence Label: EZ-L560
FEATURES             Location/Qualifiers
     source          1..12464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    205..322
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        337..734
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             764..784
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1673..1920
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1689..1707)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        2091..2122
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2179..4821
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     promoter        complement(6592..6779)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        complement(7105..7123)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7144..7160)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7144..7160)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7157..7179)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7168..7184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7192..7198,7199..7216,7217..7222))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7237..7258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7375..7392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7546..8134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7626..7645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8305..9096,9097..9165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8928..8947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9166..9270)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9354..9372
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9410..9432)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9532..9551
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9907..10362)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10044..10065)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10256..10275
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10488..10510
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10502..10519
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10503..10519
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10526..10545
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10526..10544
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(11204..11369)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     11290..11314
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(11385..12092)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(11425..11445)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     12040..12068
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(join(12134..12464,1..111))
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(12140..12163)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    join(12458..12464,1..111)
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 gtgcggcgcg aggcacatct gcgtttcagg aacgcgaccg gtgaagacga ggacgcacgg
       61 aggagagtct tccttcggag ggctgtcacc cgctcggcgg cttctaatcc gtttttgcat
      121 ttatatatct gttaatagat caaaaatcat ccaatttttc ctcttcataa ccataaaagc
      181 tagtattgta gaatctttat tgttcggagc agtgcggcgc gaggcacatc tgcgtttcag
      241 gaacgcgacc ggtgaagacg aggacgcacg gaggagagtc ttccttcgga gggctgtcac
      301 ccgctcggcg gcttctaatc cgcccggaaa cagctgatag cttcaaaatg tttctactcc
      361 ttttttactc ttccagattt tctcggactc cgcgcatcgc cgtaccactt caaaacaccc
      421 aagcacagca tactaaattt cccctctttc ttcctctagg gtgtcgttaa ttacccgtac
      481 taaaggtttg gaaaagaaaa aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca
      541 ataaaaattt ttatcacgtt tctttttctt gaaaattttt tttttgattt ttttctcttt
      601 cgatgacctc ccattgatat ttaagttaat aaacggtctt caatttctca agtttcagtt
      661 tcatttttct tgttctatta caactttttt tacttcttgc tcattagaaa gaaagcatag
      721 caatctaatc taagttttgg cggccgctaa aatcatggct agcccgaaaa aaaagagaaa
      781 agtagcccca ccaacggacg tttcacttgg tgacgaatta catttagacg gtgaggatgt
      841 agctatggca catgccgatg ctcttgatga ttttgatctg gacatgttag gtgatgggga
      901 ttctcccggc cccggattta caccacatga ttctgctccg tatggcgcgt tggacatggc
      961 agatttcgaa tttgaacaga tgtttactga tgcacttggt atcgacgaat atggaggtga
     1021 attcggagca gatgatacta gagttgaagt ccaacctcca gcccaatggg tattggattt
     1081 gatcgaggcc tcacccatag caagtgttgt tagtgatccc agattggctg ataatccttt
     1141 gattgctatc aaccaggcct ttaccgatct aactgggtat tctgaggaag aatgtgttgg
     1201 gcgtaattgt aggttcttac agggatctgg tactgaaccc tggctaacag ataagatccg
     1261 tcaaggtgtt cgtgagcata aacccgtgtt ggttgagatt ttgaattaca aaaaggatgg
     1321 tactcctttt cgtaatgcag ttttggtggc accaatctat gatgacgatg atgaattact
     1381 atacttcctt ggtagccaag tggaagtgga tgacgatcaa ccgaacatgg gcatggcccg
     1441 tagagaacgt gctgcggaga tgttaaaaac tctttcacca cgtcaattag aagtcacaac
     1501 tctggttgca tcaggcctaa gaaataaaga agtggctgcc agactgggtc tttcagaaaa
     1561 aactgtcaaa atgcacagag gtttggtaat ggaaaaatta aacttaaaaa cgagtgcaga
     1621 tttagttaga attgccgtag aagcaggtat ttaagtcgac ctcgagtaat gatcatgtaa
     1681 ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa
     1741 ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta
     1801 agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt
     1861 aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc
     1921 ggccttatcg ccgggtacgt gagttcgcca gcttcgagta ggtagccttt agtccatgcg
     1981 ttataggtag cctttagtcc atgcgttata ggtagccttt agtccatgcg ttataggtag
     2041 cctttagtcc atgcgttata ggtagccttt agtccatgct taagagacac tagagggtat
     2101 ataatggaag ctcgacttcc agcttggcaa tccggtactg ttggtaaagc caccgcggcc
     2161 gctaaaatca tggctagcat gaagctactg tcttctatcg aacaagcatg cgatatttgc
     2221 cgacttaaaa agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac
     2281 aactgggagt gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg
     2341 acagaagtgg aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga
     2401 gaagaccttg acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca
     2461 ggattatttg tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg
     2521 gagactgata tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa
     2581 gagagtagta acaaaggtca aagacagttg actgtatcga ttgactcggc agctcatcat
     2641 gataactcca caattccgtt ggattttatg cccagggatg ctcttcatgg atttgattgg
     2701 tctgaagagg atgacatgtc ggatggcttg cccttcctga aaacggaccc caacaataat
     2761 gggttctttg gcgacggttc tctcttatgt attcttcgat ctattggctt taaaccggaa
     2821 aattacacga actctaacgt taacaggctc ccgaccatga ttacggatag atacacgttg
     2881 gcttctagat ccacaacatc ccgtttactt caaagttatc tcaataattt tcacccctac
     2941 tgccctatcg tgcactcacc gacgctaatg atgttgtata ataaccagat tgaaatcgcg
     3001 tcgaaggatc aatggcaaat cctttttaac tgcatattag ccattggagc ctggtgtata
     3061 gagggggaat ctactgatat agatgttttt tactatcaaa atgctaaatc tcatttgacg
     3121 agcaaggtct tcgagtcagg ttccataatt ttggtgacag ccctacatct tctgtcgcga
     3181 tatacacagt ggaggcagaa aacaaatact agctataatt ttcacagctt ttccataaga
     3241 atggccatat cattgggctt gaatagggac ctcccctcgt ccttcagtga tagcagcatt
     3301 ctggaacaaa gacgccgaat ttggtggtct gtctactctt gggagatcca attgtccctg
     3361 ctttatggtc gatccatcca gctttctcag aatacaatct ccttcccttc ttctgtcgac
     3421 gatgtgcagc gtaccacaac aggtcccacc atatatcatg gcatcattga aacagcaagg
     3481 ctcttacaag ttttcacaaa aatctatgaa ctagacaaaa cagtaactgc agaaaaaagt
     3541 cctatatgtg caaaaaaatg cttgatgatt tgtaatgaga ttgaggaggt ttcgagacag
     3601 gcaccaaagt ttttacaaat ggatatttcc accaccgctc taaccaattt gttgaaggaa
     3661 cacccttggc tatcctttac aagattcgaa ctgaagtgga aacagttgtc tcttatcatt
     3721 tatgtattaa gagatttttt cactaatttt acccagaaaa agtcacaact agaacaggat
     3781 caaaatgatc atcaaagtta tgaagttaaa cgatgctcca tcatgttaag cgatgcagca
     3841 caaagaactg ttatgtctgt aagtagctat atggacaatc ataatgtcac cccatatttt
     3901 gcctggaatt gttcttatta cttgttcaat gcagtcctag tacccataaa gactctactc
     3961 tcaaactcaa aatcgaatgc tgagaataac gagaccgcac aattattaca acaaattaac
     4021 actgttctga tgctattaaa aaaactggcc acttttaaaa tccagacttg tgaaaaatac
     4081 attcaagtac tggaagaggt atgtgcgccg tttctgttat cacagtgtgc aatcccatta
     4141 ccgcatatca gttataacaa tagtaatggt agcgccatta aaaatattgt cggttctgca
     4201 actatcgccc aataccctac tcttccggag gaaaatgtca acaatatcag tgttaaatat
     4261 gtttctcctg gctcagtagg gccttcacct gtgccattga aatcaggagc aagtttcagt
     4321 gatctagtca agctgttatc taaccgtcca ccctctcgta actctccagt gacaatacca
     4381 agaagcacac cttcgcatcg ctcagtcacg ccttttctag ggcaacagca acagctgcaa
     4441 tcattagtgc cactgacccc gtctgctttg tttggtggcg ccaattttaa tcaaagtggg
     4501 aatattgctg atagctcatt gtccttcact ttcactaaca gtagcaacgg tccgaacctc
     4561 ataacaactc aaacaaattc tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat
     4621 gataacttca tgaataatga aatcacggct agtaaaattg atgatggtaa taattcaaaa
     4681 ccactgtcac ctggttggac ggaccaaact gcgtataacg cgtttggaat cactacaggg
     4741 atgtttaata ccactacaat ggatgatgta tataactatc tattcgatga tgaagatacc
     4801 ccaccaaacc caaaaaaaga gctcgagtaa tgatcatctc tgcttttgtg cgcgtatgtt
     4861 tatgtatgta cctctctctc tatttctatt tttaaaccac cctctcaata aaataaaaat
     4921 aataaagtat ttttaaggaa aagacgtgtt taagcactga ctttatctac tttttgtacg
     4981 ttttcattga tataatgtgt tttgtctctc ccttttctac gaaaatttca aaaattgacc
     5041 aaaaaaagga atatatatac gaaaaactat tatatttata tatcatagtg ttgataaaaa
     5101 atgtttatcc attggaccgt gtatcatatt tatcgccggc gaataaatta aggcgcgcca
     5161 acatatccgg aggtacccaa agccgaatcc accacggtca ccgttgtttc tgaagaaacc
     5221 accacggccg ccaccgttgt tgaaaccacc acgaggagca gaatcgcctc ttctgccacc
     5281 accgttacgg atgtgaggtg gaacgtaacc accacgtctt tgttgccctt caggcatgga
     5341 aacttgctct gctaactcag tcatattgga tatatgtatt actttcttta ttttttgtag
     5401 tttagtcttc tatttctttt atagtttggt atctttttgc aaaaaattta cctctatatc
     5461 gtgttcgtgc gtatttcttc ctctataata tgaaaagaaa ggcagtagtc ttggttttct
     5521 attattccaa atattacaag aatacccaga accacaagaa gcagaagaaa ctaactgctt
     5581 tatatactta atttcccgcc acaagctcat cgcccagaag tttttttcag tttttcacct
     5641 gacaatctgg cagctcgctg aaaaattttc acctggggtc gcctgctacg tagagtgatc
     5701 acgtttatga atagcacatt atatagtggc ccagacggtc tgatggtagt tcatgtagtt
     5761 aagaataaat actctgtttg tgtgatttct atgtgtacgt tatctatata aatattgttg
     5821 atggtggtag ctgtgggctg tgttctatgc taggacaccc ttagtggatg gaacgtcatc
     5881 gcgacccgta ggtgttcttg cctctctgat ggcgatggcg agcgccttga aagctgattc
     5941 ggagcggtgg tggtcgttgg tgccccgcag acagtccaca tgcatggtga tacgcgcctc
     6001 agtggcgaaa ctctccaaga agtgcgggat catctctgtg gatagctggc ctatgcgttc
     6061 tcgcttcagg cccagctcca ccacggcgaa aggcctattg gagaagtcaa ccacagcgcg
     6121 gctcagcgcc tcgtccagtg gtgcaaaccc atgcccgaac ctcttgatac cacgcacgga
     6181 gccaagcgcc tccttgaacg cttggcccag cgcaatccca cagtcctcaa cagtgtggtg
     6241 gtcatcgatg tgcaagtccc ctatacactc caggatcaga gaccacccgc catgcttcgc
     6301 cagcgcagtc aacatgtggt ccaaaaaccc aactccagtg tgcacatgga tggtctgccc
     6361 atcaccacta gcatattcct tgcccagtat gctctcccgt acagaaacag acccaccgtc
     6421 aagatccagc gccagctgta tattcgtctc ctgcgtaacc ctcttaacaa acgccatcct
     6481 ttgctcgatg cttctctttg cttcaattat ctcaacttta agtaaacctg aagtaaacta
     6541 gaactagatt aaatcagaag gcatccgcag ctttatatac atatagagcc tctttgcctt
     6601 cgtttatctt gcctgctcat tttttagtat attcttcgaa gaaatcacat tactttatat
     6661 aatgtataat tcattatgtg ataatgccaa tcgctaagaa aaaaaaagag tcatccgcta
     6721 ggtggaaaaa aaaaaatgaa aatcattacc gaggcataaa aaaatataga gtgtactaga
     6781 ggaggccaag agtaatagaa aaagaaaatt gcgggaaagg actgtgttat gacttccctg
     6841 actaatgccg tgttcaaacg atacctggca gtgactccta gcgctcacca agctcttaaa
     6901 acgagaatta agaaaaagtc gtcatctttc gataagtttt tcccacagca aagcaatagt
     6961 agaaaaaaac aatgggaaac gttgaatgaa gacaaagcgt cgtggtttaa aaggaaatac
     7021 gctcacgtac atgctaggga acaggaccgt gcagcggcca gcgtggccgt ttaaacattt
     7081 aaatggccgg cccagctttt gttcccttta gtgagggtta attccgagct tggcgtaatc
     7141 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7201 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7261 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7321 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7381 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7741 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7981 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8341 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8701 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8761 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9301 cctcggcgcg tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     9361 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     9421 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     9481 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     9541 agcagattgt actgagagtg caccatacca ccttttcaat tcatcatttt ttttttattc
     9601 ttttttttga tttcggtttc cttgaaattt ttttgattcg gtaatctccg aacagaagga
     9661 agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatattgg gtcagcagta
     9721 cagaaccctc gacgaagttg taagtggtgg atcagatatc atcattgttg gcagaggact
     9781 tttcgccaag ggtagagatc ctaatactaa actcacaaat tagagcttca atttaattat
     9841 atcagttatt accctatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     9901 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     9961 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
    10021 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
    10081 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
    10141 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
    10201 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
    10261 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
    10321 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
    10381 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    10441 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    10501 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggggcca gcgtggccgt
    10561 ttaaacattt aaatggccgg ccgctgccac cgttgtttcc accgaagaaa ccaccgttgc
    10621 cgtaaccacc acgacggttg ttgctaaaga agctgccacc gccacggcca ccgttgtagc
    10681 cgccgttgtt gttattgtag ttgctactgt tatttctggc acttcttggt tttcctctta
    10741 agtgaggagg aacataacca ttctcgttgt tgtcgttgat gcttaaattt tgcacttgtt
    10801 cgctcagttc agccataata tgaaatgctt ttcttgttgt tcttacggaa taccacttgc
    10861 cacctatcac cacaactaac tttttcccgt tcctccatct cttttatatt ttttttctgg
    10921 agttcaagag aaaaaaaaag aaaaagcaaa aagaaaaaag gaaagcgcgc ctcgttcaga
    10981 atgacacgta tagaatgatg cattaccttg tcatcttcag tatcatactg ttcgtataca
    11041 tacttactga cattcatagg tatacatata tacacatgta tatatatcgt atgctgcagc
    11101 tttaaataat cggtgtcaag atctgagctc caccgcggtg gcggccgctt atttactcag
    11161 gatcccgcat gttgcaggtg ggagttccag ctcccggcga taagagcgac ctcatgctat
    11221 acctgagaaa gcaacctgac ctacaggaaa gagttactca agaataagaa ttttcgtttt
    11281 aaaacctaag agtcacttta aaatttgtat acacttattt tttttataac ttatttaata
    11341 ataaaaatca taaatcataa gaaattcgct gatcattact cgagtttgta tagttcatcc
    11401 atgccatgtg taatcccagc agctgttaca aactcaagaa ggaccatgtg gtctctcttt
    11461 tcgttgggat ctttcgaaag ggcagattgt gtggacaggt aatggttgtc tggtaaaagg
    11521 acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg aacgcttcca
    11581 tcttcaatgt tgtgtctaat tttgaagtta actttgattc cattcttttg tttgtctgcc
    11641 atgatgtata cattgtgtga gttatagttg tattccaatt tgtgtccaag aatgtttcca
    11701 tcttctttaa aatcaatacc ttttaactcg attctattaa caagggtatc accttcaaac
    11761 ttgacttcag cacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt
    11821 acataacctt cgggcatggc actcttgaaa aagtcatgcc gtttcatatg atctgggtat
    11881 cttgaaaagc attgaacacc ataagtgaaa gtagtgacaa gtgttggcca tggaacaggt
    11941 agttttccag tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca
    12001 ccctctccac tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt
    12061 gggacaactc cagtgaaaag ttcttctcct ttgtcgacgc tagccatgat tttagcggcc
    12121 gctatagttt tttctccttg acgttaaagt atagaggtat attaacaatt ttttgttgat
    12181 acttttatta catttgaata agaagtaata caaaccgaaa atgttgaaag tattagttaa
    12241 agtggttatg cagtttttgc atttatatat ctgttaatag atcaaaaatc atcgcttcgc
    12301 tgattaatta ctactaaaat aaggctaaaa aactaatcgc attatcatcc tatggttgtt
    12361 aatttgattc gttcatttga aggttttacc aaccaggtta ctgccaattt ttcctcttca
    12421 taaccataaa agctagtatt gtagaatctt tattgttcgg agca
//
