LOCUS       sequence-340157-        9217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral bicistronic vector for the constitutive co-expression of 
            2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Simoneschi D, Rona G, Zhou N, Jeong YT, Jiang S, Milletti G, Arbini 
            AA, O'Sullivan A, Wang AA, Nithikasem S, Keegan S, Siu Y, 
            Cianfanelli V, Maiani E, Nazio F, Cecconi F, Boccalatte F, Fenyo D, 
            Jones DR, Busino L, Pagano M
  TITLE     CRL4(AMBRA1) is a master regulator of D-type cyclins.
  JOURNAL   Nature. 2021 Apr;592(7856):789-793. doi: 10.1038/s41586-021-03445-y.
            Epub 2021 Apr 14.
  PUBMED    33854235
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03445-y"; journalName: "Nature"; date: 
            "2021-04"; volume: "592"; issue: "7856"; pages: "789-793"
COMMENT     Sequence Label: pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..9217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2815..2838
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2854..2877
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2893..2916
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2953..2976
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    3888..4460
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3911..4462
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4055..4072)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             4462..5172
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4607..4625)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4875..4894
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5186..5774
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5239..5259)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5657..5668)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5981..6614
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6742..6758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6742..6758)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6755..6777)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6766..6782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6790..6796,6797..6814,6815..6820))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6835..6856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6973..6990)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7144..7732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7224..7243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7903..8694,8695..8763))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8526..8545
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8764..8868)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8916..9050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8953..8972)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9007..9026
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcag catggactac
     2821 aaggacgacg atgacaaggt accaggatct ggggattata aagatgatga tgataaaggg
     2881 tcggccgcca gctggagcca ccctcagttc gagaagggag gaggaagcgg cggaggcagc
     2941 ggaggaggaa gctggagcca cccgcagttc gagaaaggag ctggatccag cagcgagctg
     3001 ctgtgttgcg aaggcacccg gcacgcgccc cgggccgggc cggacccgcg gctgctgggg
     3061 gaccagcgtg tcctgcagag cctgctccgc ctggaggagc gctacgtacc ccgcgcctcc
     3121 tacttccagt gcgtgcagcg ggagatcaag ccgcacatgc ggaagatgct ggcttactgg
     3181 atgctggagg tatgtgagga gcagcgctgt gaggaggaag tcttccccct ggccatgaac
     3241 tacctggatc gctacctgtc ttgcgtcccc acccgaaagg cgcagttgca gctcctgggt
     3301 gcggtctgca tgctgctggc ctccaagctg cgcgagacca cgcccctgac catcgaaaaa
     3361 ctgtgcatct acaccgacca cgctgtctct ccccgccagt tgcgggactg ggaggtgctg
     3421 gtcctaggga agctcaagtg ggacctggct gctgtgattg cacatgattt cctggccttc
     3481 attctgcacc ggctctctct gccccgtgac cgacaggcct tggtcaaaaa gcatgcccag
     3541 acctttttgg ccctctgtgc tacagattat acctttgcca tgtacccgcc atccatgatc
     3601 gccacgggca gcattggggc tgcagtgcaa ggcctgggtg cctgctccat gtccggggat
     3661 gagctcacag agctgctggc agggatcact ggcactgaag tggactgcct gcgggcctgt
     3721 caggagcaga tcgaagctgc actcagggag agcctcaggg aagccgctca gaccagctcc
     3781 agcccagcgc ccaaagcccc ccggggctcc agcagccaag ggcccagcca gaccagcact
     3841 cctacagatg tcacagccat acacctgtag tgcggccgcg gatcccgccc ctctccctcc
     3901 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     3961 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     4021 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     4081 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     4141 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     4201 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     4261 atagttgtgg aaagagtcaa atggctcacc tcaagcgtat tcaacaaggg gctgaaggat
     4321 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     4381 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     4441 tttgaaaaac acgatgataa tatggtgagc aagggcgagg aggataacat ggccatcatc
     4501 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     4561 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     4621 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     4681 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     4741 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     4801 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     4861 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     4921 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     4981 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     5041 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     5101 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     5161 ctgtacaagt gaacgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     5221 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5281 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5341 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5401 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     5461 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5521 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5581 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5641 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5701 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5761 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     5821 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     5881 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     5941 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc
     6001 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     6061 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     6121 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     6181 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     6241 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     6301 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     6361 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     6421 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6481 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6541 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6601 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6661 gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag
     6721 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6781 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6841 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6901 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6961 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7021 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7081 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7141 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7201 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7261 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7321 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7381 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7441 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7501 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7561 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7621 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7681 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7741 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7801 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7861 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7921 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7981 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8041 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8101 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8161 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8221 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8281 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8341 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8401 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8461 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8521 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8581 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8641 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8701 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8761 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8821 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8881 aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg
     8941 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9001 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
     9061 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
     9121 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
     9181 attgtatttg ttaaatatgt actacaaact tagtagt
//
