LOCUS       sequence-340162-        9243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral bicistronic vector for the constitutive co-expression of 
            2xFLAG-2xSTREP-tagged cyclin D1(P287S) and mCherry in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Simoneschi D, Rona G, Zhou N, Jeong YT, Jiang S, Milletti G, Arbini 
            AA, O'Sullivan A, Wang AA, Nithikasem S, Keegan S, Siu Y, 
            Cianfanelli V, Maiani E, Nazio F, Cecconi F, Boccalatte F, Fenyo D, 
            Jones DR, Busino L, Pagano M
  TITLE     CRL4(AMBRA1) is a master regulator of D-type cyclins.
  JOURNAL   Nature. 2021 Apr;592(7856):789-793. doi: 10.1038/s41586-021-03445-y.
            Epub 2021 Apr 14.
  PUBMED    33854235
REFERENCE   2  (bases 1 to 9243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03445-y"; journalName: "Nature"; date: 
            "2021-04"; volume: "592"; issue: "7856"; pages: "789-793"
COMMENT     Sequence Label: pLVX-2xFLAG-2xSTREP-CCND1(P287S)-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..9243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2815..2838
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2854..2877
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2893..2916
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2953..2976
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     regulatory      3593..3602
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3914..4486
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3937..4488
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4081..4098)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             4488..5198
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4633..4651)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4901..4920
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5212..5800
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5265..5285)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5683..5694)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6007..6640
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6768..6784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6768..6784)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6781..6803)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6792..6808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6816..6822,6823..6840,6841..6846))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6861..6882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6999..7016)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7170..7758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7250..7269)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7929..8720,8721..8789))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8552..8571
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8790..8894)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8942..9076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8979..8998)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9033..9052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcag catggactac
     2821 aaggacgacg atgacaaggt accaggatct ggggattata aagatgatga tgataaaggg
     2881 tcggccgcca gctggagcca ccctcagttc gagaagggag gaggaagcgg cggaggcagc
     2941 ggaggaggaa gctggagcca cccgcagttc gagaaaggag ctggatccag cagcgaacac
     3001 cagctcctgt gctgcgaagt ggaaaccatc cgccgcgcgt accccgatgc caacctcctc
     3061 aacgaccggg tgctgcgggc catgctgaag gcggaggaga cctgcgcgcc ctcggtgtcc
     3121 tacttcaaat gtgtgcagaa ggaggtcctg ccgtccatgc ggaagatcgt cgccacctgg
     3181 atgctggagg tctgcgagga acagaagtgc gaggaggagg tcttcccgct ggccatgaac
     3241 tacctggacc gcttcctgtc gctggagccc gtgaaaaaga gccgcctgca gctgctgggg
     3301 gccacttgca tgttcgtggc ctctaagatg aaggagacca tccccctgac ggccgagaag
     3361 ctgtgcatct acaccgacaa ctccatccgg cccgaggagc tgctgcaaat ggagctgctc
     3421 ctggtgaaca agctcaagtg gaacctggcc gcaatgaccc cgcacgattt cattgaacac
     3481 ttcctctcca aaatgccaga ggcggaggag aacaaacaga tcatccgcaa acacgcgcag
     3541 accttcgttg ccctctgtgc cacagatgtg aagttcattt ccaatccgcc ctccatggtg
     3601 gcagcgggga gcgtggtggc cgcagtgcaa ggcctgaacc tgaggagccc caacaacttc
     3661 ctgtcctact accgcctcac acgcttcctc tccagagtga tcaagtgtga cccagactgc
     3721 ctccgggcct gccaggagca gatcgaagcc ctgctggagt caagcctgcg ccaggcccag
     3781 cagaacatgg accccaaggc cgccgaggag gaggaagagg aggaggagga ggtggacctg
     3841 gcttgcacaa gcaccgacgt gcgggacgtg gacatctgac tcgagactag ttctagagcg
     3901 gccgcggatc ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     3961 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     4021 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     4081 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     4141 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     4201 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     4261 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctcacctcaa
     4321 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     4381 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc
     4441 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg gtgagcaagg
     4501 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     4561 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     4621 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     4681 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     4741 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     4801 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     4861 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     4921 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     4981 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     5041 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     5101 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     5161 gccactccac cggcggcatg gacgagctgt acaagtgaac gcgtctggaa caatcaacct
     5221 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5281 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5341 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5401 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5461 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5521 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5581 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     5641 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5701 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5761 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc
     5821 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     5881 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     5941 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga
     6001 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     6061 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     6121 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     6181 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     6241 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     6301 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     6361 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     6421 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     6481 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6541 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6601 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     6661 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     6721 tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6781 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6841 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6901 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6961 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7501 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7561 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7621 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7681 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7741 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7801 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7861 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7921 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7981 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8041 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8101 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8161 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8221 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8281 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8341 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8401 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8461 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8521 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8581 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8641 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8701 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8761 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8821 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8881 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8941 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9001 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9061 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     9121 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     9181 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     9241 agt
//
