LOCUS       sequence-340174-       11178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon 
            optimized Cas9 under the control of the TEF1 promoter and 
            terminator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Huang MY, Joshi MB, Boucher MJ, Lee S, Loza LC, Gaylord EA, Doering 
            TL, Madhani HD
  TITLE     Short homology-directed repair using optimized Cas9 in the pathogen 
            Cryptococcus neoformans enables rapid gene deletion and tagging.
  JOURNAL   Genetics. 2021 Oct 23. pii: 6409193. doi: 10.1093/genetics/iyab180.
  PUBMED    34791226
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2021 Oct 23. pii: 6409193. doi: 10.1093/genetics/iyab180."
COMMENT     Sequence Label: pBHM2403
FEATURES             Location/Qualifiers
     source          1..11178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     419..436
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    553..574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(589..594,595..612,613..619)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    627..643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     632..654
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     651..667
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     651..667
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        688..706
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     734..750
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     735..751
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1630..1656)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1657..1677)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1684..1704)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1720..4104)
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(7129..7148)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7130..7148)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7155..7172)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7155..7171)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7164..7186)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7312..7767
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7399..7418)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7609..7630
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7898..8701)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8702..8917)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8941..8960)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9060..9082
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9120..9138)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    9179..9682
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        9719..9823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9824..9892,9893..10684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10042..10061)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10855..11178,1..265)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
       61 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      121 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      181 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      241 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      301 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      361 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
      421 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
      481 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      541 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      601 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      661 ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctggt
      721 accgggcccc ccctcgaggt cgacggtatc gatcgttcaa tctcatagtc ttctaaagat
      781 cccccgttcc caaacatgac attcaacatc ttcgcttcga cttccacctc gaccaacccc
      841 ttttcttcca aatccactcg acgtgcgcca tccccctctt cctgccccat ccctcctccg
      901 tttgccccgc caactcttcc caggttgatg aagggggtgc cctggcccga caagcgtcga
      961 ggcgaggtaa gtgtgtggct gagattcaag gcattaacga ggacgtgtga gcaggtggaa
     1021 gtgcggttgc ggtaggaagg acgaggtcag ggatgcgtag agacatcggt aattctgtac
     1081 atctgtttct tgcttctttc cccgttcttt ccatgcaaca aactattatt acaacatgaa
     1141 ctctcttagc atgacagtcc acagaaccga gatggtcagt gcatgtcgag attatttcgt
     1201 ggtgatctcc aggaacgact gaaaaaagga gtgacggatt aaggaagacg tgaccgacta
     1261 aaggaaagga aggatggaag gaacgatatc aatgcgatgg tggcgacctg agaatgagta
     1321 aatagttgaa agcactaaaa taaagcagca acgaaggtta ttccaagtta tagctctcgt
     1381 gcaaatttca acagtgatgg ccattttatt gttgcaagag acgtcgtcag ccgagaaata
     1441 ttcgatttcg cggacaacta tcgggaatcc aataacggac aaacgacatg gaatgatata
     1501 tcacggcaat gaagattttg aagcgcagag gtatacatca gcaaataaga cacaaaacgt
     1561 aagaagcaca agtagcaaca gtcatccgat gcatattacg cgtaagaagg gtcactacat
     1621 aagtaattag gcgtagtcgg ggacgtcgta ggggtagacc tttcgcttct tcttgggggg
     1681 gtagaccttt cgcttcttct tggggggacc ggaaccaccg tcaccaccga gctgggagag
     1741 gtcgattcgg gtctcgtaga gaccggtgat ggactggtgg atgagggtgg cgtcgaggac
     1801 ctccttggtg gaggtgtatc gctttcggtc gatggtggtg tcgaagtact tgaaggcggc
     1861 gggggcaccg aggttggtga gggtgaagag gtggatgatg ttctcggcct gctctcggat
     1921 gggcttgtct cggtgcttgt tgtaggcgga gaggaccttg tcgaggttgg cgtcggcgag
     1981 gatgactcgc ttggagaact cggagatctg ctcgatgatc tcgtcgaggt agtgcttgtg
     2041 ctgctcgacg aagagctgct tctgctcgtt gtcctcgggg gaacccttga gcttctcgta
     2101 gtgggaggcg aggtagagga agttgacgta cttggagggg agggcgagct cgttaccctt
     2161 ctggagctca ccggcggagg cgagcattcg ctttcgaccg ttctcgagct cgaagaggga
     2221 gtacttgggg agcttgatga tgaggtcctt cttgacctcc ttgtaaccct tggcctcgag
     2281 gaagtcgatg gggttcttct cgaaggagga tcgctccatg atggtgatac cgaggagctc
     2341 cttgacggac ttgagcttct tggacttacc cttctcgacc ttggcgacga cgaggacgga
     2401 gtaggcgacg gtgggggagt cgaaaccacc gtacttcttg gggtcccagt ccttctttcg
     2461 ggcgatgagc ttgtcggagt ttcgcttggg gaggatggac tccttggaga aaccaccggt
     2521 ctggacctcg gtcttcttga cgatgttgac ctggggcatg gagaggacct ttcggacggt
     2581 ggcgaagtct cgacccttgt cccagacgat ctcaccggtc tcaccgttgg tctcgatgag
     2641 gggtcgcttt cggatctcac cgttggcgag ggtgatctcg gtcttgaaga agttcatgat
     2701 gttggagtag aagaagtact tggcggtggc cttaccgatc tcctgctcgg acttggcgat
     2761 catctttcgg acgtcgtaga ccttgtagtc accgtagacg aactcggact cgagcttggg
     2821 gtacttcttg atgagggcgg taccgacgac ggcgttgagg taggcgtcgt gggcgtggtg
     2881 gtagttgttg atctctcgga ccttgtagaa ctggaagtcc tttcggaagt cggagacgag
     2941 cttggacttg agggtgatga ccttgacctc tcggatgagc ttgtcgttct cgtcgtactt
     3001 ggtgttcatt cgggagtcga ggatctgggc gacgtgcttg gtgatctgtc gggtctcgac
     3061 gagctgtcgc ttgatgaaac cggccttgtc gagctcggag agaccacctc gctcggcctt
     3121 ggtgaggttg tcgaactttc gctgggtgat gagcttggcg ttgaggagct gtcgccagta
     3181 gttcttcatc ttcttgacga cctcctcgga ggggacgttg tcggacttac ctcggttctt
     3241 gtcggatcgg gtgaggacct tgttgtcgat ggagtcgtcc ttgaggaagg actgggggac
     3301 gatgtggtcg acgtcgtagt cggagagtcg gttgatgtcg agctcctggt cgacgtacat
     3361 gtctcgaccg ttctggaggt agtagaggta gagcttctcg ttctggagct gggtgttctc
     3421 gacggggtgc tccttgagga tctgggaacc gagctccttg ataccctcct cgattcgctt
     3481 cattcgctct cgggagttct tctgaccctt ctgggtggtc tggttctctc gggccatctc
     3541 gatgacgatg ttctcgggct tgtgtcgacc catgaccttg acgagctcgt cgacgacctt
     3601 gacggtctgg aggataccct tcttgatggc gggggaaccg gcgaggttgg cgatgtgctc
     3661 gtggagggag tcaccctgac cggagacctg ggccttctgg atgtcctcct tgaaggtgag
     3721 ggagtcgtcg tggatgagct gcatgaagtt tcggttggcg aaaccgtcgg acttgaggaa
     3781 gtcgaggatg gtcttaccgg actgcttgtc tcggataccg ttgatgagct ttcgggagag
     3841 tcgaccccaa ccggtgtatc gtcgtcgctt gagctgcttc atgaccttgt cgtcgaagag
     3901 gtgggcgtag gtcttgagtc gctcctcgat catctctcgg tcctcgaaga gggtgagggt
     3961 gaggacgatg tcctcgagga tgtcctcgtt ctcctcgttg tcgaggaagt ccttgtcctt
     4021 gatgatcttg aggaggtcgt ggtaggtacc gagggaggcg ttgaatcggt cctcgacacc
     4081 ggagatctcg acggagtcga aacactcgat cttcttgaag tagtcctcct tgagctgctt
     4141 gacggtgacc tttcggttgg tcttgaagag gaggtcgacg atggccttct tctgctcacc
     4201 ggagaggaag gcgggctttc gcataccctc ggtgacgtac ttgaccttgg tgagctcgtt
     4261 gtagacggtg aagtactcgt agaggaggga gtgcttgggg aggaccttct cgttggggag
     4321 gttcttgtcg aagttggtca ttcgctcgat gaaggactgg gcggaggcac ccttgtcgac
     4381 gacctcctcg aagttccagg gggtgatggt ctcctcggac tttcgggtca tccaggcgaa
     4441 tcgggagtta cctcgggcga ggggaccgac gtagtagggg attcggaagg tgaggatctt
     4501 ctcgatcttc tctcggttgt ccttgaggaa ggggtagaag tcctcctgtc gtcggaggat
     4561 ggcgtggagc tcaccgaggt ggatctggtg ggggatggaa ccgttgtcga aggttcgctg
     4621 ctttcggagg aggtcctctc ggttgagctt gacgaggagc tcctcggtac cgtccatctt
     4681 ctcgaggatg ggcttgatga acttgtagaa ctcctcctgg gaggcaccac cgtcgatgta
     4741 accggcgtaa ccgttcttgg actggtcgaa gaagatctcc tgtcgggaga tgttagtgga
     4801 gtaacatgtt gcgggaaacg gggttacata ccttgtactt ctcggggagc tgctgtcgga
     4861 cgagggcctt gaggagggtg aggtcctggt ggtgctcgtc gtatcgcttg atcatggagg
     4921 cggagagggg ggccttggtg atctcggtgt tgactcggag gatgtcggag aggaggatgg
     4981 cgtcggagag gttcttggcg gcgaggaaga ggtcggcgta ctggtcaccg atctgggcga
     5041 ggaggttgtc gaggtcgtcg tcgtaggtgt ccttggagag ctggagcttg gcgtcctcgg
     5101 cgaggtcgaa gttggacttg aagttggggg tgagaccgag ggagagggcg atgaggttac
     5161 cgaagagacc gttcttcttc tcaccgggga gctgggcgat gaggttctcg agtcgtcggg
     5221 acttggagag tcgggcggag aggatggcct tggcgtcgac accggaggcg ttgatggggt
     5281 tctcctcgaa gagctggttg taggtctgga cgagctggat gaagagcttg tcgacgtcgg
     5341 agttgtcggg gttgaggtca ccctcgatga ggaagtgacc tcggaacttg atcatgtggg
     5401 cgagggcgag gtagatgagt cggaggtcgg ccttgtcggt ggagtcgacg agcttctttc
     5461 ggaggtggta gatggtgggg tacttctcgt ggtaggcgac ctcgtcgacg atgttaccga
     5521 agatggggtg tcgctcgtgc ttcttgtcct cctcgacgag gaaggactcc tcgagtcggt
     5581 ggaagaagga gtcgtcgacc ttggccatct cgttggagaa gatctcctgg aggtaacaga
     5641 ttcggttctt tcgtcgggtg tatcgtcgtc gggcggttcg cttgagtcgg gtggcctcgg
     5701 cggtctcacc ggagtcgaag aggagggcac cgatgaggtt cttcttgatg gagtgtcggt
     5761 cggtgttacc gaggaccttg aacttcttgg aggggacctt gtactcgtcg gtgatgacgg
     5821 cccaaccgac ggagttggta ccgatgtcga gaccgatgga gtacttcttg tccattttga
     5881 agttttctgt ggagatcgtt agcatgtgcg aagaggcaat gataggacaa taaagtgtta
     5941 gggcgttgtg agttggaagg gcatgtatcc gatgggttga aaattttcaa gagccaccaa
     6001 atttttttct tcaccacaca caagcccatg ttgagctcag tagtggcaaa acagtactct
     6061 gcagtcgacg caacagatga gggcaaaagc atggaagaca tcgccaaaaa caagacagaa
     6121 tgatgtgggt ggtcgaccat gctcagctcc actctgcccc gacttcacct gcagaagcca
     6181 ccagcgagaa ttgttttacg tgccctacga ctggcgcgga ttttcaaagg tgtcaaaaga
     6241 ctttgggtac tcacctaggt gttttggtta aagaatgtag agataaagaa ggtggtggga
     6301 gaaaggaatg tgcgagggat ttaattcttt ccggttgatg ggcagggcgg attctcggat
     6361 taattcagaa aatcccacaa tttttttcga aacctccaga atgtgaaaag ctcactggaa
     6421 atttcatccg tatcaaactc cgtttttcac cgagtgtggc acatgggact taggaggcaa
     6481 gaaatgtgag gaggatggag cgaccgcaac aacggagaaa tttaaagtgg aggtcgttcc
     6541 caggcacgtc ttcgcaccaa aaattcaacg ggtggcaaga ggcccctgcc gtctaaacat
     6601 gtgggagtgg ctcatcctca caccgagcag gccaatgcat atgataagag tcgtccagaa
     6661 gccgacgtgc atgaatggag cttcacaaaa tgcgaaatta gaagaagacg agatgcccat
     6721 gatcttctgg gaagggggcg aggcaacttg cttggcgaat ggggctgaat attggggcgt
     6781 tcgctaatgg ccatgacgct tgttcatggc agggtagaac acgacattat tgcttgtgcg
     6841 tgggggcttc tggctgcttt ttgtacattg cgcgaagggt tgccatgatg agactgctgt
     6901 attatgctct tattcttttg tttaaaggga gtgatacttg acataccgat gatagatagt
     6961 taagaagcgc ccgcaggtgg gtcaaccaca cttgcgacca ttgttaatca ttcagaacgg
     7021 tcgacaaggt cagtgtaaga tcattcgcta tggagcttgc atagtagcca caatcttgta
     7081 ccgtatagca tagaatggcg gccgccaccg cggtggagct ccaattcgcc ctatagtgag
     7141 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7201 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7261 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     7321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7381 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7441 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7501 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7621 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7681 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7741 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     7801 cggtatttca caccgcatag ggtaataact gatataatta aattgaagct ctaatttgtg
     7861 agtttagtat acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc
     7921 tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc
     7981 cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat
     8041 ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg
     8101 tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc
     8161 cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag
     8221 tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg
     8281 ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg
     8341 cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga
     8401 ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg
     8461 ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat
     8521 gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg
     8581 tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata
     8641 gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca
     8701 tgatttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag aatactgggc
     8761 aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct
     8821 tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa
     8881 atcaaaaaaa agaataaaaa aaaaatgatg aattgaaaag gtggtatggt gcactctcag
     8941 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     9001 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     9061 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     9121 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgga
     9181 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt
     9241 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag
     9301 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa
     9361 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc
     9421 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac
     9481 aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat
     9541 ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta ttttttcttt
     9601 aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa tttaaattat
     9661 aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg gaaatgtgcg
     9721 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9781 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9841 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9901 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9961 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    10021 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
    10081 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    10141 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    10201 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    10261 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    10321 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    10381 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    10441 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10501 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10561 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10621 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10681 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10741 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10801 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10861 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10921 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10981 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    11041 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    11101 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    11161 gcggtcgggc tgaacggg
//
