LOCUS       sequence-340178-        2408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway attB4/attB5 entry clone for baculovirus expression using the
            AcMNPV polyhedrin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D
  TITLE     Combinatorial assembly of clone libraries using site-specific 
            recombination.
  JOURNAL   Methods Mol Biol. 2014;1116:193-208. doi: 
            10.1007/978-1-62703-764-8_14.
  PUBMED    24395366
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2014"; volume: "1116:193-208. doi"; pages: " 
            10.1007/978-1-62703-764-8_14"
COMMENT     Sequence Label: C453-E18: attB4-polyhedrinp>-attB5
FEATURES             Location/Qualifiers
     source          1..2408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    51..146
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(149..240)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(207..224)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     protein_bind    complement(306..401)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      449..535
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      627..654
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(789..1377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(869..888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1520..2329)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2243..2262
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2321..2340
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 catcagagat tttgagacac gggccctgcg ccctgcagct ctagagctcc aaataatgat
       61 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gcttttttat
      121 aatgccaact ttgtatacaa aagttggcat ttataggttt ttttattaca aaactgttac
      181 gaaaacagta aaatacttat ttatttgcga gatggttatc attttaatta tctccatgat
      241 ctattaatat tccggagtat acgtagccaa ccactagaac tatagctaga gtcctgggcg
      301 aacgccaact tttctataca aagttggcat tataaaaaag cattgcttat caatttgttg
      361 caacgaacag gtcactatca gtcaaaataa aatcattatt tggagctcca tggtagcgtt
      421 aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cggaagactg
      481 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
      541 gggagcggat ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
      601 ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg acggatggcc tttttgcgtt
      661 tctacaaact cttcctggct agcggtaata cggttatcca cagaatcagg ggataacgca
      721 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      781 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      841 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      901 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      961 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1021 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1081 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1141 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1201 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1261 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1321 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1381 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1441 attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc cggtgttaca
     1501 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     1561 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     1621 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     1681 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     1741 aatcaccatg agtgacgact gaatccggcg agaatggcaa aagtttatgc atttctttcc
     1801 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     1861 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     1921 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1981 tttcacctga atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag
     2041 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     2101 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     2161 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaag cgatagattg
     2221 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     2281 tgttggaatt taatcgcggc ctcgacgttt cccgttgaat atggctcata acaccccttg
     2341 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     2401 caatgtaa
//
