LOCUS       sequence-340210-        8619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 8619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: MAC-DPP4
FEATURES             Location/Qualifiers
     source          1..8619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        173..552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        553..756
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     707..727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    758..776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    779..797
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     807..831
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(999..1349,1350..1352,1353..1961)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1962..1988
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2019..2042
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2079..2102
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    2115..2139
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             4243..4251
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(4447..4471)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4509..4526)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4515..4739
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4872..4891)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5023..5070
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5078..6100
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5078..6100
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6230..6351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6267..6286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6400..6416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6400..6416)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6413..6435)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6424..6440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6448..6454,6455..6472,6473..6478))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6493..6514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6631..6648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6802..7390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6882..6901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7561..8352,8353..8421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8184..8203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8422..8526)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(8601..8619,1))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
       61 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      121 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      181 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      241 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      301 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      361 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      421 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      481 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      541 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      601 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      661 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      721 aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc
      781 cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat cgcctggaga
      841 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccggact
      901 ctagcgttta aacttaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat
      961 tctgcagata tccagcacag tggcggccgc tcgagaccat gaaggacaac accgtgcccc
     1021 tgaagctgat cgccctgctg gccaacggcg agttccactc tggcgagcag ctgggcgaga
     1081 cactgggcat gagcagagcc gccatcaaca agcacatcca gaccctgcgg gactggggcg
     1141 tggacgtgtt cacagtgccc ggcaagggct acagcctgcc cgagcccatc cagctgctga
     1201 acgccaagca gatcctgggc cagctggatg gcggatctgt ggccgtgctg cccgtgatcg
     1261 acagcaccaa ccagtatctg ctcgaccgga tcggcgagct gaagtccggc gacgcctgta
     1321 tcgccgagta ccagcaggcc ggcagaggcg gcaggggcag aaagtggttc agccccttcg
     1381 gcgccaacct gtacctgagc atgttctgga gactggaaca gggcccagcc gccgctattg
     1441 gcctgagcct ggtcatcggc atcgtgatgg ccgaggtgct gcggaagctg ggcgccgaca
     1501 aagtgcgcgt gaagtggccc aacgacctgt atctgcagga ccggaagctg gccggcatcc
     1561 tggtggaact gaccggcaaa acaggcgacg ccgcccagat cgtgatcggc gctggcatca
     1621 acatggccat gcggcgggtg gaagagagcg tggtcaacca gggctggatc accctgcagg
     1681 aagccggcat caacctggac cggaacaccc tggccgccat gctgatcaga gagctgagag
     1741 ccgccctgga actgttcgag caggaaggcc tggcccccta cctgagcaga tgggagaagc
     1801 tggacaactt catcaaccgg cccgtgaaac tgatcatcgg cgacaaagag atcttcggca
     1861 tcagccgggg catcgacaag cagggggccc tgctcctgga acaggacggc atcatcaagc
     1921 cctggatggg cggcgagatc agcctgcgga gcgccgagaa gtacccatac gatgttcctg
     1981 actatgccgg taccgagctc ggatccacca tggctagctg gagccacccg cagttcgaga
     2041 aaggtggagg ttccggaggt ggatcgggag gtggatcgtg gagccacccg cagttcgaaa
     2101 aagcggccga tatcacaagt ttgtacaaaa aagcaggcta caaaatgaag acaccgtgga
     2161 aggttcttct gggactgctg ggtgctgctg cgcttgtcac catcatcacc gtgcccgtgg
     2221 ttctgctgaa caaaggcaca gatgatgcta cagctgacag tcgcaaaact tacactctaa
     2281 ctgattactt aaaaaatact tatagactga agttatactc cttaagatgg atttcagatc
     2341 atgaatatct ctacaaacaa gaaaataata tcttggtatt caatgctgaa tatggaaaca
     2401 gctcagtttt cttggagaac agtacatttg atgagtttgg acattctatc aatgattatt
     2461 caatatctcc tgatgggcag tttattctct tagaatacaa ctacgtgaag caatggaggc
     2521 attcctacac agcttcatat gacatttatg atttaaataa aaggcagctg attacagaag
     2581 agaggattcc aaacaacaca cagtgggtca catggtcacc agtgggtcat aaattggcat
     2641 atgtttggaa caatgacatt tatgttaaaa ttgaaccaaa tttaccaagt tacagaatca
     2701 catggacggg gaaagaagat ataatatata atggaataac tgactgggtt tatgaagagg
     2761 aagtcttcag tgcctactct gctctgtggt ggtctccaaa cggcactttt ttagcatatg
     2821 cccaatttaa cgacacagaa gtcccactta ttgaatactc cttctactct gatgagtcac
     2881 tgcagtaccc aaagactgta cgggttccat atccaaaggc aggagctgtg aatccaactg
     2941 taaagttctt tgttgtaaat acagactctc tcagctcagt caccaatgca acttccatac
     3001 aaatcactgc tcctgcttct atgttgatag gggatcacta cttgtgtgat gtgacatggg
     3061 caacacaaga aagaatttct ttgcagtggc tcaggaggat tcagaactat tcggtcatgg
     3121 atatttgtga ctatgatgaa tccagtggaa gatggaactg cttagtggca cggcaacaca
     3181 ttgaaatgag tactactggc tgggttggaa gatttaggcc ttcagaacct cattttaccc
     3241 ttgatggtaa tagcttctac aagatcatca gcaatgaaga aggttacaga cacatttgct
     3301 atttccaaat agataaaaaa gactgcacat ttattacaaa aggcacctgg gaagtcatcg
     3361 ggatagaagc tctaaccagt gattatctat actacattag taatgaatat aaaggaatgc
     3421 caggaggaag gaatctttat aaaatccaac ttagtgacta tacaaaagtg acatgcctca
     3481 gttgtgagct gaatccggaa aggtgtcagt actattctgt gtcattcagt aaagaggcga
     3541 agtattatca gctgagatgt tccggtcctg gtctgcccct ctatactcta cacagcagcg
     3601 tgaatgataa agggctgaga gtcctggaag acaattcagc tttggataaa atgctgcaga
     3661 atgtccagat gccctccaaa aaactggact tcattatttt gaatgaaaca aaattttggt
     3721 atcagatgat cttgcctcct cattttgata aatccaagaa atatcctcta ctattagatg
     3781 tgtatgcagg cccatgtagt caaaaagcag acactgtctt cagactgaac tgggccactt
     3841 accttgcaag cacagaaaac attatagtag ctagctttga tggcagagga agtggttacc
     3901 aaggagataa gatcatgcat gcaatcaaca gaagactggg aacatttgaa gttgaagatc
     3961 aaattgaagc agccagacaa ttttcaaaaa tgggatttgt ggacaacaaa cgaattgcaa
     4021 tttggggctg gtcatatgga gggtacgtaa cctcaatggt cctgggatcg ggaagtggcg
     4081 tgttcaagtg tggaatagcc gtggcgcctg tatcccggtg ggagtactat gactcagtgt
     4141 acacagaacg ttacatgggt ctcccaactc cagaagacaa ccttgaccat tacagaaatt
     4201 caacagtcat gagcagagct gaaaatttta aacaagttga gtacctcctt attcatggaa
     4261 cagcagatga taacgttcac tttcagcagt cagctcagat ctccaaagcc ctggtcgatg
     4321 ttggagtgga tttccaggca atgtggtata ctgatgaaga ccatggaata gctagcagca
     4381 cagcacacca acatatatat acccacatga gccacttcat aaaacaatgt ttctctttac
     4441 cttagaaccc agctttcttg tacaaagtgg tgacgtaagc taggggcccg tttaaacccg
     4501 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4561 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4621 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4681 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4741 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     4801 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4861 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4921 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4981 cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag
     5041 ttcctattct ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac
     5101 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     5161 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     5221 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     5281 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     5341 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     5401 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     5461 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     5521 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     5581 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     5641 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     5701 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     5761 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     5821 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     5881 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     5941 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     6001 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     6061 aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga
     6121 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6181 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     6241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6361 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6421 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6481 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6541 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6601 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6661 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7201 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8521 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     8581 cccctatggt gcactctcag tacaatctgc tctgatgcc
//
