LOCUS       sequence-340214-        9222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9222)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 9222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: MAC-ANPEP
FEATURES             Location/Qualifiers
     source          1..9222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..47)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(201..789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(281..300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(960..1751,1752..1820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1583..1602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1821..1925)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2000..2019)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2191..2570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2571..2774
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2725..2745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2776..2794
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2797..2815
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2825..2849
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3017..3367,3368..3370,3371..3979)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3980..4006
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4037..4060
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4097..4120
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    4133..4157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7068..7092)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7130..7147)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7136..7360
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7493..7512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7644..7691
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7699..8721
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7699..8721
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8851..8972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8888..8907)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8942..8961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9021..9037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9021..9037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9034..9056)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9045..9061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9069..9075,9076..9093,9094..9099))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9114..9135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
       61 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      601 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     1141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1561 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     1621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     1681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1921 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     1981 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     2041 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     2101 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     2161 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     2221 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     2281 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     2341 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     2401 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     2461 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     2521 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     2581 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     2641 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     2701 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     2761 atataagcag agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc
     2821 gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2881 tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa cttaagcttg
     2941 gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg
     3001 gcggccgctc gagaccatga aggacaacac cgtgcccctg aagctgatcg ccctgctggc
     3061 caacggcgag ttccactctg gcgagcagct gggcgagaca ctgggcatga gcagagccgc
     3121 catcaacaag cacatccaga ccctgcggga ctggggcgtg gacgtgttca cagtgcccgg
     3181 caagggctac agcctgcccg agcccatcca gctgctgaac gccaagcaga tcctgggcca
     3241 gctggatggc ggatctgtgg ccgtgctgcc cgtgatcgac agcaccaacc agtatctgct
     3301 cgaccggatc ggcgagctga agtccggcga cgcctgtatc gccgagtacc agcaggccgg
     3361 cagaggcggc aggggcagaa agtggttcag ccccttcggc gccaacctgt acctgagcat
     3421 gttctggaga ctggaacagg gcccagccgc cgctattggc ctgagcctgg tcatcggcat
     3481 cgtgatggcc gaggtgctgc ggaagctggg cgccgacaaa gtgcgcgtga agtggcccaa
     3541 cgacctgtat ctgcaggacc ggaagctggc cggcatcctg gtggaactga ccggcaaaac
     3601 aggcgacgcc gcccagatcg tgatcggcgc tggcatcaac atggccatgc ggcgggtgga
     3661 agagagcgtg gtcaaccagg gctggatcac cctgcaggaa gccggcatca acctggaccg
     3721 gaacaccctg gccgccatgc tgatcagaga gctgagagcc gccctggaac tgttcgagca
     3781 ggaaggcctg gccccctacc tgagcagatg ggagaagctg gacaacttca tcaaccggcc
     3841 cgtgaaactg atcatcggcg acaaagagat cttcggcatc agccggggca tcgacaagca
     3901 gggggccctg ctcctggaac aggacggcat catcaagccc tggatgggcg gcgagatcag
     3961 cctgcggagc gccgagaagt acccatacga tgttcctgac tatgccggta ccgagctcgg
     4021 atccaccatg gctagctgga gccacccgca gttcgagaaa ggtggaggtt ccggaggtgg
     4081 atcgggaggt ggatcgtgga gccacccgca gttcgaaaaa gcggccgata tcacaagttt
     4141 gtacaaaaaa gcaggctaca aaatggccaa gggcttctat atttccaagt ccctgggcat
     4201 cctggggatc ctcctgggcg tggcagccgt gtgcacaatc atcgcactgt cagtggtgta
     4261 ctcccaggag aagaacaaga acgccaacag ctcccccgtg gcctccacca ccccgtccgc
     4321 ctcagccacc accaaccccg cctcggccac caccttggac caaagtaaag cgtggaatcg
     4381 ttaccgcctc cccaacacgc tgaaacccga ttcctaccgg gtgacgctga gaccgtacct
     4441 cacccccaat gacaggggcc tgtacgtttt taagggctcc agcaccgtcc gtttcacctg
     4501 caaggaggcc actgacgtca tcatcatcca cagcaagaag ctcaactaca ccctcagcca
     4561 ggggcacagg gtggtcctgc gtggtgtggg aggctcccag ccccccgaca ttgacaagac
     4621 tgagctggtg gagcccaccg agtacctggt ggtgcacctc aagggctccc tggtgaagga
     4681 cagccagtat gagatggaca gcgagttcga gggggagttg gcagatgacc tggcgggctt
     4741 ctaccgcagc gagtacatgg agggcaatgt cagaaaggtg gtggccacta cacagatgca
     4801 ggctgcagat gcccggaagt ccttcccatg cttcgatgag ccggccatga aggccgagtt
     4861 caacatcacg cttatccacc ccaaggacct gacagccctg tccaacatgc ttcccaaagg
     4921 tcccagcacc ccacttccag aagaccccaa ctggaatgtc actgagttcc acaccacgcc
     4981 caagatgtcc acgtacttgc tggccttcat tgtcagtgag ttcgactacg tggagaagca
     5041 ggcatccaat ggtgtcttga tccggatctg ggcccggccc agtgccattg cggcgggcca
     5101 cggcgattat gccctgaacg tgacgggccc catccttaac ttctttgctg gtcattatga
     5161 cacaccctac ccactcccaa aatcagacca gattggcctg ccagacttca acgccggcgc
     5221 catggagaac tggggactgg tgacctaccg ggagaactcc ctgctgttcg accccctgtc
     5281 ctcctccagc agcaacaagg agcgggtggt cactgtgatt gctcatgagc tggcccacca
     5341 gtggttcggg aacctggtga ccatagagtg gtggaatgac ctgtggctga acgagggctt
     5401 cgcctcctac gtggagtacc tgggtgctga ctatgcggag cccacctgga acttgaaaga
     5461 cctcatggtg ctgaatgatg tgtaccgcgt gatggcagtg gatgcactgg cctcctccca
     5521 cccgctgtcc acacccgcct cggagatcaa cacgccggcc cagatcagtg agctgtttga
     5581 cgccatctcc tacagcaagg gcgcctcagt cctcaggatg ctctccagct tcctgtccga
     5641 ggacgtattc aagcagggcc tggcgtccta cctccacacc tttgcctacc agaacaccat
     5701 ctacctgaac ctgtgggacc acctgcagga ggctgtgaac aaccggtcca tccaactccc
     5761 caccaccgtg cgggacatca tgaaccgctg gaccctgcag atgggcttcc cggtcatcac
     5821 ggtggatacc agcacgggga ccctttccca ggagcacttc ctccttgacc ccgattccaa
     5881 tgttacccgc ccctcagaat tcaactacgt gtggattgtg cccatcacat ccatcagaga
     5941 tggcagacag cagcaggact actggctgat agatgtaaga gcccagaacg atctcttcag
     6001 cacatcaggc aatgagtggg tcctgctgaa cctcaatgtg acgggctatt accgggtgaa
     6061 ctacgacgaa gagaactgga ggaagattca gactcagctg cagagagacc actcggccat
     6121 ccctgtcatc aatcgggcac agatcattaa tgacgccttc aacctggcca gtgcccataa
     6181 ggtccctgtc actctggcgc tgaacaacac cctcttcctg attgaagaga gacagtacat
     6241 gccctgggag gccgccctga gcagcctgag ctacttcaag ctcatgtttg accgctccga
     6301 ggtctatggc cccatgaaga actacctgaa gaagcaggtc acacccctct tcattcactt
     6361 cagaaataat accaacaact ggagggagat cccagaaaac ctgatggacc agtacagcga
     6421 ggttaatgcc atcagcaccg cctgctccaa cggagttcca gagtgtgagg agatggtctc
     6481 tggccttttc aagcagtgga tggagaaccc caataataac ccgatccacc ccaacctgcg
     6541 gtccaccgtc tactgcaacg ctatcgccca gggcggggag gaggagtggg acttcgcctg
     6601 ggagcagttc cgaaatgcca cactggtcaa tgaggctgac aagctccggg cagccctggc
     6661 ctgcagcaaa gagttgtgga tcctgaacag gtacctgagc tacaccctga acccggactt
     6721 aatccggaag caggacgcca cctctaccat catcagcatt accaacaacg tcattgggca
     6781 aggtctggtc tgggactttg tccagagcaa ctggaagaag ctttttaacg attatggtgg
     6841 tggctcgttc tccttctcca acctcatcca ggcagtgaca cgacgattct ccaccgagta
     6901 tgagctgcag cagctggagc agttcaagaa ggacaacgag gaaacaggct tcggctcagg
     6961 cacccgggcc ctggagcaag ccctggagaa gacgaaagcc aacatcaagt gggtgaagga
     7021 gaacaaggag gtggtgctcc agtggttcac agaaaacagc aaatagaacc cagctttctt
     7081 gtacaaagtg gtgacgtaag ctaggggccc gtttaaaccc gctgatcagc ctcgactgtg
     7141 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7201 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     7261 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     7321 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     7381 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7441 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7501 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7561 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7621 tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc tctagaaagt
     7681 ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     7741 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     7801 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     7861 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     7921 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     7981 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     8041 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     8101 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     8161 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     8221 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     8281 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     8341 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     8401 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     8461 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     8521 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     8581 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     8641 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     8701 cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc cgccttctat
     8761 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8821 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8881 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8941 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     9001 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9061 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9121 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9181 tgccagctgc attaatgaat cggccaacgc gcggggagag gc
//
