LOCUS       sequence-340217-        8601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8601)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 8601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: MAC-TFRC
FEATURES             Location/Qualifiers
     source          1..8601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..20)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(174..762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(254..273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(933..1724,1725..1793))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1556..1575
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1794..1898)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1973..1992)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2164..2543
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2544..2747
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2698..2718
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2770..2788
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2798..2822
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2990..3340,3341..3343,3344..3952)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3953..3979
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4010..4033
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4070..4093
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    4106..4130
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5075..5086)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6420..6444)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6482..6499)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6488..6712
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6845..6864)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6996..7043
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7051..8073
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7051..8073
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8203..8324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8240..8259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8294..8313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8373..8389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8373..8389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8386..8408)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8397..8413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8421..8427,8428..8445,8446..8451))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8466..8487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
       61 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      121 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      181 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      241 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      301 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      361 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      421 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      481 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      541 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      601 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      661 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      721 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      781 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      841 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      901 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      961 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1021 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1081 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1141 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1201 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1261 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1321 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1381 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1441 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1501 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1561 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1621 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1681 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1741 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1801 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1861 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1921 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     1981 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     2041 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     2101 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     2161 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     2221 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2281 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2341 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2401 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2461 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2521 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2581 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2641 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2701 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg
     2761 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
     2821 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     2881 gcctccggac tctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag
     2941 tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagacca tgaaggacaa
     3001 caccgtgccc ctgaagctga tcgccctgct ggccaacggc gagttccact ctggcgagca
     3061 gctgggcgag acactgggca tgagcagagc cgccatcaac aagcacatcc agaccctgcg
     3121 ggactggggc gtggacgtgt tcacagtgcc cggcaagggc tacagcctgc ccgagcccat
     3181 ccagctgctg aacgccaagc agatcctggg ccagctggat ggcggatctg tggccgtgct
     3241 gcccgtgatc gacagcacca accagtatct gctcgaccgg atcggcgagc tgaagtccgg
     3301 cgacgcctgt atcgccgagt accagcaggc cggcagaggc ggcaggggca gaaagtggtt
     3361 cagccccttc ggcgccaacc tgtacctgag catgttctgg agactggaac agggcccagc
     3421 cgccgctatt ggcctgagcc tggtcatcgg catcgtgatg gccgaggtgc tgcggaagct
     3481 gggcgccgac aaagtgcgcg tgaagtggcc caacgacctg tatctgcagg accggaagct
     3541 ggccggcatc ctggtggaac tgaccggcaa aacaggcgac gccgcccaga tcgtgatcgg
     3601 cgctggcatc aacatggcca tgcggcgggt ggaagagagc gtggtcaacc agggctggat
     3661 caccctgcag gaagccggca tcaacctgga ccggaacacc ctggccgcca tgctgatcag
     3721 agagctgaga gccgccctgg aactgttcga gcaggaaggc ctggccccct acctgagcag
     3781 atgggagaag ctggacaact tcatcaaccg gcccgtgaaa ctgatcatcg gcgacaaaga
     3841 gatcttcggc atcagccggg gcatcgacaa gcagggggcc ctgctcctgg aacaggacgg
     3901 catcatcaag ccctggatgg gcggcgagat cagcctgcgg agcgccgaga agtacccata
     3961 cgatgttcct gactatgccg gtaccgagct cggatccacc atggctagct ggagccaccc
     4021 gcagttcgag aaaggtggag gttccggagg tggatcggga ggtggatcgt ggagccaccc
     4081 gcagttcgaa aaagcggccg atatcacaag tttgtacaaa aaagcaggct ccaccatgat
     4141 ggatcaagct agatcagcat tctctaactt gtttggtgga gaaccattgt catatacccg
     4201 gttcagcctg gctcggcaag tagatggcga taacagtcat gtggagatga aacttgctgt
     4261 agatgaagaa gaaaatgctg acaataacac aaaggccaat gtcacaaaac caaaaaggtg
     4321 tagtggaagt atctgctatg ggactattgc tgtgatcgtc tttttcttga ttggatttat
     4381 gattggctac ttgggctatt gtaaaggggt agaaccaaaa actgagtgtg agagactggc
     4441 aggaaccgag tctccagtga gggaggagcc aggagaggac ttccctgcag cacgtcgctt
     4501 atattgggat gacctgaaga gaaagttgtc ggagaaactg gacagcacag acttcaccgg
     4561 caccatcaag ctgctgaatg aaaattcata tgtccctcgt gaggctggat ctcaaaaaga
     4621 tgaaaatctt gcgttgtatg ttgaaaatca atttcgtgaa tttaaactca gcaaagtctg
     4681 gcgtgatcaa cattttgtta agattcaggt caaagacagc gctcaaaact cggtgatcat
     4741 agttgataag aacggtagac ttgtttacct ggtggagaat cctgggggtt atgtggcgta
     4801 tagtaaggct gcaacagtta ctggtaaact ggtccatgct aattttggta ctaaaaaaga
     4861 ttttgaggat ttatacactc ctgtgaatgg atctatagtg attgtcagag cagggaaaat
     4921 cacctttgca gaaaaggttg caaatgctga aagcttaaat gcaattggtg tgttgatata
     4981 catggaccag actaaatttc ccattgttaa cgcagaactt tcattctttg gacatgctca
     5041 tctggggaca ggtgaccctt acacacctgg attcccttcc ttcaatcaca ctcagtttcc
     5101 accatctcgg tcatcaggat tgcctaatat acctgtccag acaatctcca gagctgctgc
     5161 agaaaagctg tttgggaata tggaaggaga ctgtccctct gactggaaaa cagactctac
     5221 atgtaggatg gtaacctcag aaagcaagaa tgtgaagctc actgtgagca atgtgctgaa
     5281 agagataaaa attcttaaca tctttggagt tattaaaggc tttgtagaac cagatcacta
     5341 tgttgtagtt ggggcccaga gagatgcatg gggccctgga gctgcaaaat ccggtgtagg
     5401 cacagctctc ctattgaaac ttgcccagat gttctcagat atggtcttaa aagatgggtt
     5461 tcagcccagc agaagcatta tctttgccag ttggagtgct ggagactttg gatcggttgg
     5521 tgccactgaa tggctagagg gatacctttc gtccctgcat ttaaaggctt tcacttatat
     5581 taatctggat aaagcggttc ttggtaccag caacttcaag gtttctgcca gcccactgtt
     5641 gtatacgctt attgagaaaa caatgcaaaa tgtgaagcat ccggttactg ggcaatttct
     5701 atatcaggac agcaactggg ccagcaaagt tgagaaactc actttagaca atgctgcttt
     5761 ccctttcctt gcatattctg gaatcccagc agtttctttc tgtttttgcg aggacacaga
     5821 ttatccttat ttgggtacca ccatggacac ctataaggaa ctgattgaga ggattcctga
     5881 gttgaacaaa gtggcacgag cagctgcaga ggtcgctggt cagttcgtga ttaaactaac
     5941 ccatgatgtt gaattgaacc tggactatga gaggtacaac agccaactgc tttcatttgt
     6001 gagggatctg aaccaataca gagcagacat aaaggaaatg ggcctgagtt tacagtggct
     6061 gtattctgct cgtggagact tcttccgtgc tacttccaga ctaacaacag atttcgggaa
     6121 tgctgagaaa acagacagat ttgtcatgaa gaaactcaat gatcgtgtca tgagagtgga
     6181 gtatcacttc ctctctccct acgtatctcc aaaagagtct cctttccgac atgtcttctg
     6241 gggctccggc tctcacacgc tgccagcttt actggagaac ttgaaactgc gtaaacaaaa
     6301 taacggtgct tttaatgaaa cgctgttcag aaaccagttg gctctagcta cttggactat
     6361 tcagggagct gcaaatgccc tctctggtga cgtttgggac attgacaatg agttttagga
     6421 cccagctttc ttgtacaaag tggtgacgta agctaggggc ccgtttaaac ccgctgatca
     6481 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6541 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6601 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6661 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6721 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6781 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6841 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6901 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6961 aaaaaacttg attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat
     7021 tctctagaaa gtataggaac ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc
     7081 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     7141 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     7201 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     7261 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     7321 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     7381 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     7441 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     7501 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     7561 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     7621 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     7681 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     7741 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     7801 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     7861 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     7921 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     7981 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     8041 cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt cgattccacc
     8101 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8161 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     8221 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8281 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     8341 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8401 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8461 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8521 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8581 cgtattgggc gctcttccgc t
//
