LOCUS       sequence-340226-       10866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 10866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: MAC-CTSS
FEATURES             Location/Qualifiers
     source          1..10866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(711..1502,1503..1571))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1334..1353
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1572..1676)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1751..1770)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1942..2321
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2322..2525
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2476..2496
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2527..2545
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2548..2566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2576..2600
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2743..2767
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3767..3791)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3838..3861
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3898..3921
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3952..3978
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3952..3978
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(3979..4326,4327..4329,4330..4938)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(4982..4999)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4988..5212
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5345..5364)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5496..5543
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5551..6573
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5551..6573
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6703..6824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6740..6759)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6794..6813
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6873..6889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6886..6908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6897..6913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6921..6927,6928..6945,6946..6951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6966..6987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7275..7863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8010..8819)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8733..8752
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8811..8830
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     8953..8972
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8953..8971
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8989..9088
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(10088..10187)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(10203..10220)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10203..10219)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10212..10234)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      10283..10369
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10461..10488
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(join(10818..10866,1..540))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
       61 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      121 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      181 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      241 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      301 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      361 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      421 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      481 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      541 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      601 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      661 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      721 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      781 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      841 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      901 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      961 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1021 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1081 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1141 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1201 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1261 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1321 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1381 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1441 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1501 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1561 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1621 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1681 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1741 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1801 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1861 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1921 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1981 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     2041 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     2101 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2161 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2221 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2281 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2341 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2401 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2461 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2521 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
     2581 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     2641 gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccgagc
     2701 tcggatccac tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
     2761 gcaggctaca aaatgaaacg gctggtttgt gtgctcttgg tgtgctcctc tgcagtggca
     2821 cagttgcata aagatcctac cctggatcac cactggcatc tctggaagaa aacctatggc
     2881 aaacaataca aggaaaagaa tgaagaagca gtacgacgtc tcatctggga aaagaatcta
     2941 aagtttgtga tgcttcacaa cctggagcat tcaatgggaa tgcactcata cgatctgggc
     3001 atgaaccacc tgggagacat gaccagtgaa gaagtgatgt ctttgatgag ttccctgaga
     3061 gttcccagcc agtggcagag aaatatcaca tataagtcaa accctaatcg aatattgcct
     3121 gattctgtgg actggagaga gaaagggtgt gttactgaag tgaaatatca aggttcttgt
     3181 ggtgcttgct gggctttcag tgctgtgggg gccctggaag cacagctgaa gctgaaaaca
     3241 ggaaagctgg tgtctctcag tgcccagaac ctggtggatt gctcaactga aaaatatgga
     3301 aacaaaggct gcaatggtgg cttcatgaca acggctttcc agtacatcat tgataacaag
     3361 ggcatcgact cagacgcttc ctatccctac aaagccatgg atcagaaatg tcaatatgac
     3421 tcaaaatatc gtgctgccac atgttcaaag tacactgaac ttccttatgg cagagaagat
     3481 gtcctgaaag aagctgtggc caataaaggc ccagtgtctg ttggtgtaga tgcgcgtcat
     3541 ccttctttct tcctctacag aagtggtgtc tactatgaac catcctgtac tcagaatgtg
     3601 aatcatggtg tacttgtggt tggctatggt gatcttaatg ggaaagaata ctggcttgtg
     3661 aaaaacagct ggggccacaa ctttggtgaa gaaggatata ttcggatggc aagaaataaa
     3721 ggaaatcatt gtgggattgc tagctttccc tcttacccag aaatcaaccc agctttcttg
     3781 tacaaagtgg ttgatatcaa ggccgccgac atcaccagcc tgtacaagaa agtggggtgg
     3841 agccatcccc agttcgagaa gggcggagga tctggcggcg gaagcggcgg aggaagctgg
     3901 tcccaccctc agtttgagaa aggcaccgag ctgggcagca ccatggccag ctacccctac
     3961 gacgtgcccg actacgccaa ggacaacacc gtgcccctga agctgatcgc cctgctggcc
     4021 aacggcgagt tccactctgg cgagcagctg ggcgagacac tgggcatgag cagagccgcc
     4081 atcaacaagc acatccagac cctgcgggac tggggcgtgg acgtgttcac agtgcccggc
     4141 aagggctaca gcctgcccga gcccatccag ctgctgaacg ccaagcagat cctgggccag
     4201 ctggatggcg gatctgtggc cgtgctgccc gtgatcgaca gcaccaacca gtatctgctc
     4261 gaccggatcg gcgagctgaa gtccggcgac gcctgtatcg ccgagtacca gcaggccggc
     4321 agaggcggca ggggcagaaa gtggttcagc cccttcggcg ccaacctgta cctgagcatg
     4381 ttctggagac tggaacaggg cccagccgcc gctattggcc tgagcctggt catcggcatc
     4441 gtgatggccg aggtgctgcg gaagctgggc gccgacaaag tgcgcgtgaa gtggcccaac
     4501 gacctgtatc tgcaggaccg gaagctggcc ggcatcctgg tggaactgac cggcaaaaca
     4561 ggcgacgccg cccagatcgt gatcggcgct ggcatcaaca tggccatgcg gcgggtggaa
     4621 gagagcgtgg tcaaccaggg ctggatcacc ctgcaggaag ccggcatcaa cctggaccgg
     4681 aacaccctgg ccgccatgct gatcagagag ctgagagccg ccctggaact gttcgagcag
     4741 gaaggcctgg ccccctacct gagcagatgg gagaagctgg acaacttcat caaccggccc
     4801 gtgaaactga tcatcggcga caaagagatc ttcggcatca gccggggcat cgacaagcag
     4861 ggggccctgc tcctggaaca ggacggcatc atcaagccct ggatgggcgg cgagatcagc
     4921 ctgcggagcg ccgagaagtg atgactcgag tctagagggc ccgtttaaac ccgctgatca
     4981 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5041 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5101 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5161 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5221 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     5281 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5341 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5401 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5461 aaaaaacttg attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat
     5521 tctctagaaa gtataggaac ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc
     5581 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     5641 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     5701 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     5761 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     5821 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     5881 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     5941 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     6001 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     6061 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     6121 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     6181 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     6241 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     6301 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     6361 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     6421 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     6481 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     6541 cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt cgattccacc
     6601 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6661 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6721 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6781 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6841 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6901 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6961 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7021 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7081 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7681 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga cgcgtaactc
     7921 acgttaaggg attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc
     7981 cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac
     8041 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     8101 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     8161 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     8221 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     8281 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     8341 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     8401 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     8461 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgtttttccg
     8521 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     8581 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     8641 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaag
     8701 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     8761 tcagcatcca tgttggaatt taatcgcggc ctcgacgttt cccgttgaat atggctcata
     8821 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
     8881 ttatcttgtg caatgtaaca tcagagattt tgagacacgg gccagagctg ccaagaaaca
     8941 gctatgacca tgtaatacga ctcactatag gggatatcag ctggatggca aataatgatt
     9001 ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg ctttcttata
     9061 atgccaactt tgtacaagaa agctgggttg atttctgggt aagagggaaa gctagcaatc
     9121 ccacaatgat ttcctttatt tcttgccatc cgaatatatc cttcttcacc aaagttgtgg
     9181 ccccagctgt ttttcacaag ccagtattct ttcccattaa gatcaccata gccaaccaca
     9241 agtacaccat gattcacatt ctgagtacag gatggttcat agtagacacc acttctgtag
     9301 aggaagaaag aaggatgacg cgcatctaca ccaacagaca ctgggccttt attggccaca
     9361 gcttctttca ggacatcttc tctgccataa ggaagttcag tgtactttga acatgtggca
     9421 gcacgatatt ttgagtcata ttgacatttc tgatccatgg ctttgtaggg ataggaagcg
     9481 tctgagtcga tgcccttgtt atcaatgatg tactggaaag ccgttgtcat gaagccacca
     9541 ttgcagcctt tgtttccata tttttcagtt gagcaatcca ccaggttctg ggcactgaga
     9601 gacaccagct ttcctgtttt cagcttcagc tgtgcttcca gggcccccac agcactgaaa
     9661 gcccagcaag caccacaaga accttgatat ttcacttcag taacacaccc tttctctctc
     9721 cagtccacag aatcaggcaa tattcgatta gggtttgact tatatgtgat atttctctgc
     9781 cactggctgg gaactctcag ggaactcatc aaagacatca cttcttcact ggtcatgtct
     9841 cccaggtggt tcatgcccag atcgtatgag tgcattccca ttgaatgctc caggttgtga
     9901 agcatcacaa actttagatt cttttcccag atgagacgtc gtactgcttc ttcattcttt
     9961 tccttgtatt gtttgccata ggttttcttc cagagatgcc agtggtgatc cagggtagga
    10021 tctttatgca actgtgccac tgcagaggag cacaccaaga gcacacaaac cagccgtttc
    10081 attttgtagc ctgctttttt gtacaaagtt ggcattataa aaaagcattg ctcatcaatt
    10141 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgggg cccgagctta
    10201 agactggccg tcgttttaca acgtcgtgac tgggaaaaca tccatgctag cgttaacgcg
    10261 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcggaag actgggcctt
    10321 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc
    10381 ggatttgaac gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac
    10441 tgccaggcat caaactaagc agaaggccat cctgacggat ggcctttttg cgtttctaca
    10501 aactcttcct ggctagcggt acgcgtatta attgcgttgc gctcactgcc cgctttccag
    10561 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    10621 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10681 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10861 cgctca
//
