LOCUS       sequence-340270-        3102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing HES3 transcription factor, part of the Human
            TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-HES3
FEATURES             Location/Qualifiers
     source          1..3102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(659..758)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(775..794)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(776..794)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(917..936)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             928..1737
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(995..1014)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1884..2472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2373..2392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2626..2643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2802..2829)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2921..3007)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3056..3078
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3070..3087
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3071..3087
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggagaaaaa gcgccgggca
      121 cgcatcaatg tgtcactgga gcagctcaag tcgctgctgg agaaacacta ctcgcaccag
      181 atccggaagc gcaaattgga gaaggccgac atcctggagt tgagcgtgaa gtacatgaga
      241 agccttcaga actccttgca agggctctgg cctgtaccca gaggagccga gcaaccgtcg
      301 ggcttccgta gctgcttgcc cggtgttagc cagctcctta ggcgtggaga tgaggtaggc
      361 agcggacttc gttgtccact agtgcccgag agcgccgccg gtagcaccat ggacagcgca
      421 gggttaggtc aggaagcacc agcgttattc cgaccttgca ctcctgctgt ctgggctcct
      481 gctcctgcag ccggaggccc gagatctcca ccacccttgc tactactccc cgaaagtctt
      541 cctggctcgt ctgctagcgt tcctccaccg cagccagctt ctagtcgttg cgctgagagt
      601 cccgggctag gtttgcgtgt gtggcgacca tggggaagtc ccggggatga cctgaactgc
      661 ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
      721 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
      781 agtgagtcgt attacatggt catagctgtt tcttggcagc tctggcccgt gtctcaaaat
      841 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
      901 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
      961 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1021 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     1081 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1141 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1201 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     1261 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1321 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1381 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1441 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     1501 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     1561 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1621 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1681 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     1741 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     1801 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     1861 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1921 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1981 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2041 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2101 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2161 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2221 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2281 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2341 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2401 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2461 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2521 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2581 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2641 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2701 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2761 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     2821 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     2881 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     2941 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     3001 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     3061 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cc
//
