LOCUS       sequence-340279-        9666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to constitutively express TEAD-binding mutant YAP 
            under control of the human PGK promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Pearson JD, Huang K, Pacal M, McCurdy SR, Lu S, Aubry A, Yu T, 
            Wadosky KM, Zhang L, Wang T, Gregorieff A, Ahmad M, Dimaras H, 
            Langille E, Cole SPC, Monnier PP, Lok BH, Tsao MS, Akeno N, Schramek
            D, Wikenheiser-Brokamp KA, Knudsen ES, Witkiewicz AK, Wrana JL, 
            Goodrich DW, Bremner R
  TITLE     Binary pan-cancer classes with distinct vulnerabilities defined by 
            pro- or anti-cancer YAP/TEAD activity.
  JOURNAL   Cancer Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016.
  PUBMED    34270926
REFERENCE   2  (bases 1 to 9666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016."
COMMENT     Sequence Label: PGKp-GFP-YAP (S94A)
FEATURES             Location/Qualifiers
     source          1..9666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1748..1865
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1914..2424
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2343..2361
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2444..2509
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4025..4049)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(4091..4665,4666..4668,4669..4677)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4258..4275)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4485..4504
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4678..4680,4681..4683,4684..5394)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4678..4680,4681..4683,4684..5394)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4723..4744)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4984..5003)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5331..5352
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5404..5457
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5458..6057
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5458..5477)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5954..5974
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6185..6418
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6490..6611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6527..6546)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6581..6600
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6651..6786
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6806..6825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6807..6825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6835..6852)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6835..6851)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6844..6866)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6993..7448
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7080..7099)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7290..7311
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7474..7578
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7579..7647,7648..8439)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7797..7816)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8610..9198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9099..9118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9352..9369
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9486..9507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9522..9527,9528..9545,9546..9552)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9560..9576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9565..9587
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9584..9600
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9584..9600
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9619..9639
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9621..9639
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacaat tctcgacctc gagacaaatg gcagtattca
     1741 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1801 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1861 attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc gagggggttg
     1921 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     1981 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     2041 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     2101 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     2161 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     2221 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     2281 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     2341 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     2401 cgaatcaccg acctctctcc ccagggggat ccaccggagc atggactaca aagaccatga
     2461 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaaga tggatcccgg
     2521 gcagcagccg ccgcctcaac cggcccccca gggccaaggg cagccgcctt cgcagccccc
     2581 gcaggggcag ggcccgccgt ccggacccgg gcaaccggca cccgcggcga cccaggcggc
     2641 gccgcaggca ccccccgccg ggcatcagat cgtgcacgtc cgcggggact cggagaccga
     2701 cctggaggcg ctcttcaacg ccgtcatgaa ccccaagacg gccaacgtgc cccagaccgt
     2761 gcccatgagg ctccggaagc tgcccgacgc cttcttcaag ccgccggagc ccaaatccca
     2821 ctcccgacag gccagtactg atgcaggcac tgcaggagcc ctgactccac agcatgttcg
     2881 agctcattcc tctccagctt ctctgcagtt gggagctgtt tctcctggga cactgacccc
     2941 cactggagta gtctctggcc cagcagctac acccacagct cagcatcttc gacagtcttc
     3001 ttttgagata cctgatgatg tacctctgcc agcaggttgg gagatggcaa agacatcttc
     3061 tggtcagaga tacttcttaa atcacatcga tcagacaaca acatggcagg accccaggaa
     3121 ggccatgctg tcccagatga acgtcacagc ccccaccagt ccaccagtgc agcagaatat
     3181 gatgaactcg gcttcaggtc ctcttcctga tggatgggaa caagccatga ctcaggatgg
     3241 agaaatttac tatataaacc ataagaacaa gaccacctct tggctagacc caaggcttga
     3301 ccctcgtttt gccatgaacc agagaatcag tcagagtgct ccagtgaaac agccaccacc
     3361 cctggctccc cagagcccac agggaggcgt catgggtggc agcaactcca accagcagca
     3421 acagatgcga ctgcagcaac tgcagatgga gaaggagagg ctgcggctga aacagcaaga
     3481 actgcttcgg caggcaatgc ggaatatcaa tcccagcaca gcaaattctc caaaatgtca
     3541 ggagttagcc ctgcgtagcc agttaccaac actggagcag gatggtggga ctcaaaatcc
     3601 agtgtcttct cccgggatgt ctcaggaatt gagaacaatg acgaccaata gctcagatcc
     3661 tttccttaac agtggcacct atcactctcg agatgagagt acagacagtg gactaagcat
     3721 gagcagctac agtgtccctc gaaccccaga tgacttcctg aacagtgtgg atgagatgga
     3781 tacaggtgat actatcaacc aaagcaccct gccctcacag cagaaccgtt tcccagacta
     3841 ccttgaagcc attcctggga caaatgtgga ccttggaaca ctggaaggag atggaatgaa
     3901 catagaagga gaggagctga tgccaagtct gcaggaagct ttgagttctg acatccttaa
     3961 tgacatggag tctgttttgg ctgccaccaa gctagataaa gaaagctttc ttacatggtt
     4021 atagacccag ctttcttgta caaagtggtg atctagctaa aacgcggcct cgaatcgaat
     4081 tccgcccccc cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     4141 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     4201 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     4261 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     4321 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     4381 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     4441 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     4501 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     4561 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     4621 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     4681 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     4741 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     4801 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4861 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     4921 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4981 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     5041 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     5101 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     5161 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     5221 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     5281 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     5341 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcagc
     5401 ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggacccatg
     5461 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     5521 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     5581 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     5641 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     5701 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     5761 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5821 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     5881 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5941 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6001 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     6061 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggtacct
     6121 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6181 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     6241 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6301 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6361 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6421 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6481 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6541 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6601 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6661 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6721 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6781 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     6841 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6901 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6961 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     7021 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     7081 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7141 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7201 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7261 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7321 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     7381 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     7441 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7501 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7561 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7621 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7681 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7741 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7801 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7861 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7921 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7981 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     8041 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     8101 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     8161 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8221 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8281 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8341 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     8401 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8461 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8521 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8581 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8641 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8701 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8761 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8821 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8881 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8941 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9001 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     9061 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     9121 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9181 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9241 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     9301 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     9361 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     9421 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     9481 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     9541 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     9601 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     9661 gcttaa
//
