LOCUS       sequence-340284-        9416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to constitutively express YAP under control of the
            EFS promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Pearson JD, Huang K, Pacal M, McCurdy SR, Lu S, Aubry A, Yu T, 
            Wadosky KM, Zhang L, Wang T, Gregorieff A, Ahmad M, Dimaras H, 
            Langille E, Cole SPC, Monnier PP, Lok BH, Tsao MS, Akeno N, Schramek
            D, Wikenheiser-Brokamp KA, Knudsen ES, Witkiewicz AK, Wrana JL, 
            Goodrich DW, Bremner R
  TITLE     Binary pan-cancer classes with distinct vulnerabilities defined by 
            pro- or anti-cancer YAP/TEAD activity.
  JOURNAL   Cancer Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016.
  PUBMED    34270926
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016."
COMMENT     Sequence Label: EFSp-GFP-YAP
FEATURES             Location/Qualifiers
     source          1..9416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     187..206
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(380..382,383..385,386..1096)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(380..382,383..385,386..1096)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(425..446)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(686..705)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1033..1054
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1106..1159
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1160..1759
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1160..1179)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1656..1676
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1887..2120
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2192..2313
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2229..2248)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2283..2302
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2353..2488
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2508..2527)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2509..2527)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2537..2554)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2537..2553)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2546..2568)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2695..3150
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2782..2801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2992..3013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3176..3280
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3281..3349,3350..4141)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3499..3518)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4312..4900
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4801..4820
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5054..5071
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5188..5209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5224..5229,5230..5247,5248..5254)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5262..5278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5267..5289
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5286..5302
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5286..5302
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5321..5341
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5323..5341
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5369..5595
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5596..5776
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5823..5948
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6441..6674
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6859..6903
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7116..7233
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7303..7514
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             7562..7627
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(9143..9167)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(9209..9416,1..367,368..370,371..379)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9376..9393)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
       61 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
      121 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
      181 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
      241 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
      301 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
      361 tgataatatg gccacaacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      421 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      481 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      541 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      601 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      661 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      721 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      781 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      841 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      901 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      961 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     1021 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1081 ggacgagctg tacaagggca gcggagaggg cagaggaagt ctgctaacat gcggtgacgt
     1141 cgaggagaat cctggaccca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     1201 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     1261 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     1321 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     1381 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     1441 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     1501 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     1561 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     1621 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     1681 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     1741 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     1801 acgaccccat gcatcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     1861 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     1921 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     1981 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     2041 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     2101 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     2161 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     2221 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2281 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     2341 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     2401 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2461 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     2521 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     2581 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     2641 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     2701 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     2761 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     2821 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     2881 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     2941 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3001 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3061 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3121 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     3181 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3241 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3301 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3361 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3421 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3481 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3541 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3601 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3661 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3721 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3781 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3841 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3901 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3961 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4021 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4081 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4141 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4201 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4261 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4321 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4381 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4441 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4501 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4561 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4621 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4681 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4741 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4801 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4861 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4921 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4981 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5041 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5101 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5161 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5221 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5281 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     5341 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     5401 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     5461 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     5521 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     5581 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5641 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5701 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5761 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     5821 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     5881 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     5941 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     6001 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     6061 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     6121 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     6181 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     6241 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     6301 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     6361 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     6421 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     6481 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     6541 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     6601 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     6661 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     6721 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     6781 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     6841 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     6901 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     6961 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     7021 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacaattc
     7081 tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     7141 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     7201 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     7261 ccactttggc cgcggctcga ggtggctccg gtgcccgtca gtgggcagag cgcacatcgc
     7321 ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt
     7381 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     7441 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     7501 ccgccagaac acagggaccg aatcaccgac ctctctcccc agggggatcc accggagcat
     7561 ggactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga
     7621 tgacaagatg gatcccgggc agcagccgcc gcctcaaccg gccccccagg gccaagggca
     7681 gccgccttcg cagcccccgc aggggcaggg cccgccgtcc ggacccgggc aaccggcacc
     7741 cgcggcgacc caggcggcgc cgcaggcacc ccccgccggg catcagatcg tgcacgtccg
     7801 cggggactcg gagaccgacc tggaggcgct cttcaacgcc gtcatgaacc ccaagacggc
     7861 caacgtgccc cagaccgtgc ccatgaggct ccggaagctg cccgactcct tcttcaagcc
     7921 gccggagccc aaatcccact cccgacaggc cagtactgat gcaggcactg caggagccct
     7981 gactccacag catgttcgag ctcattcctc tccagcttct ctgcagttgg gagctgtttc
     8041 tcctgggaca ctgaccccca ctggagtagt ctctggccca gcagctacac ccacagctca
     8101 gcatcttcga cagtcttctt ttgagatacc tgatgatgta cctctgccag caggttggga
     8161 gatggcaaag acatcttctg gtcagagata cttcttaaat cacatcgatc agacaacaac
     8221 atggcaggac cccaggaagg ccatgctgtc ccagatgaac gtcacagccc ccaccagtcc
     8281 accagtgcag cagaatatga tgaactcggc ttcaggtcct cttcctgatg gatgggaaca
     8341 agccatgact caggatggag aaatttacta tataaaccat aagaacaaga ccacctcttg
     8401 gctagaccca aggcttgacc ctcgttttgc catgaaccag agaatcagtc agagtgctcc
     8461 agtgaaacag ccaccacccc tggctcccca gagcccacag ggaggcgtca tgggtggcag
     8521 caactccaac cagcagcaac agatgcgact gcagcaactg cagatggaga aggagaggct
     8581 gcggctgaaa cagcaagaac tgcttcggca ggcaatgcgg aatatcaatc ccagcacagc
     8641 aaattctcca aaatgtcagg agttagccct gcgtagccag ttaccaacac tggagcagga
     8701 tggtgggact caaaatccag tgtcttctcc cgggatgtct caggaattga gaacaatgac
     8761 gaccaatagc tcagatcctt tccttaacag tggcacctat cactctcgag atgagagtac
     8821 agacagtgga ctaagcatga gcagctacag tgtccctcga accccagatg acttcctgaa
     8881 cagtgtggat gagatggata caggtgatac tatcaaccaa agcaccctgc cctcacagca
     8941 gaaccgtttc ccagactacc ttgaagccat tcctgggaca aatgtggacc ttggaacact
     9001 ggaaggagat ggaatgaaca tagaaggaga ggagctgatg ccaagtctgc aggaagcttt
     9061 gagttctgac atccttaatg acatggagtc tgttttggct gccaccaagc tagataaaga
     9121 aagctttctt acatggttat agacccagct ttcttgtaca aagtggtgat ctagctaaaa
     9181 cgcggcctcg aatcgaattc cgcccccccc cctctccctc ccccccccct aacgttactg
     9241 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     9301 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     9361 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaag
//
