LOCUS       sequence-340288-        9269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to constitutively express TAZ/WWTR1 under control 
            of the human PGK promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Pearson JD, Huang K, Pacal M, McCurdy SR, Lu S, Aubry A, Yu T, 
            Wadosky KM, Zhang L, Wang T, Gregorieff A, Ahmad M, Dimaras H, 
            Langille E, Cole SPC, Monnier PP, Lok BH, Tsao MS, Akeno N, Schramek
            D, Wikenheiser-Brokamp KA, Knudsen ES, Witkiewicz AK, Wrana JL, 
            Goodrich DW, Bremner R
  TITLE     Binary pan-cancer classes with distinct vulnerabilities defined by 
            pro- or anti-cancer YAP/TEAD activity.
  JOURNAL   Cancer Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016.
  PUBMED    34270926
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016."
COMMENT     Sequence Label: PGKp-GFP-TAZ
FEATURES             Location/Qualifiers
     source          1..9269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             103..283
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    330..455
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    948..1181
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1366..1410
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1623..1740
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1789..2299
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2218..2236
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2319..2348
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    join(3569..4143,4144..4146,4147..4155)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3736..3753)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3963..3982
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4156..4158,4159..4161,4162..4872)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4156..4158,4159..4161,4162..4872)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4201..4222)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4462..4481)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4809..4830
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4882..4935
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4936..5535
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4936..4955)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5432..5452
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5663..5896
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5968..6089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6005..6024)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6059..6078
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6129..6264
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6284..6303)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6285..6303)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6313..6330)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6313..6329)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6322..6344)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6471..6926
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6558..6577)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6768..6789
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6952..7056
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7057..7125,7126..7917)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7275..7294)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8088..8676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8577..8596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8830..8847
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8964..8985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9000..9005,9006..9023,9024..9030)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9038..9054
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9043..9065
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9062..9078
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9062..9078
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9097..9117
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9099..9117
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(9145..9269,1..102)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
       61 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
      121 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      181 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      241 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      301 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      361 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      421 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      481 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
      541 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
      601 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
      661 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
      721 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
      781 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
      841 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
      901 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
      961 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1021 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1081 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1141 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1201 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1261 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1321 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1381 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1441 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1501 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1561 cacctcccaa ccccgagggg acaattctcg acctcgagac aaatggcagt attcatccac
     1621 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1681 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1741 cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggg ggttggggtt
     1801 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     1861 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     1921 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     1981 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     2041 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     2101 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     2161 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     2221 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     2281 caccgacctc tctccccagg gggatccacc ggagcatgga acaaaaactc atctcagaag
     2341 aggatctgac cggtaatccg gcctcggcgc cccctccgct cccgccgcct gggcagcaag
     2401 tgatccacgt cacgcaggac ctagacacag acctcgaagc cctcttcaac tctgtcatga
     2461 atccgaagcc tagctcgtgg cggaagaaga tcctgccgga gtctttcttt aaggagcctg
     2521 attcgggctc gcactcgcgc cagtccagca ccgactcgtc gggcggccac ccggggcctc
     2581 gactggctgg gggtgcccag catgtccgct cgcactcgtc gcccgcgtcc ctgcagctgg
     2641 gcaccggcgc gggtgctgcg ggtagccccg cgcagcagca cgcgcacctc cgccagcagt
     2701 cctacgacgt gaccgacgag ctgccactgc ccccgggctg ggagatgacc ttcacggcca
     2761 ctggccagag gtacttcctc aatcacatag aaaaaatcac cacatggcaa gaccctagga
     2821 aggcgatgaa tcagcctctg aatcatatga acctccaccc tgccgtcagt tccacaccag
     2881 tgcctcagag gtccatggca gtatcccagc caaatctcgt gatgaatcac caacaccagc
     2941 agcagatggc ccccagtacc ctgagccagc agaaccaccc cactcagaac ccacccgcag
     3001 ggctcatgag tatgcccaat gcgctgacca ctcagcagca gcagcagcag aaactgcggc
     3061 ttcagagaat ccagatggag agagaaagga ttcgaatgcg ccaagaggag ctcatgaggc
     3121 aggaagctgc cctctgtcga cagctcccca tggaagctga gactcttgcc ccagttcagg
     3181 ctgctgtcaa cccacccacg atgaccccag acatgagatc catcactaat aatagctcag
     3241 atcctttcct caatggaggg ccatatcatt cgagggagca gagcactgac agtggcctgg
     3301 ggttagggtg ctacagtgtc cccacaactc cggaggactt cctcagcaat gtggatgaga
     3361 tggatacagg agaaaacgca ggacaaacac ccatgaacat caatccccaa cagacccgtt
     3421 tccctgattt ccttgactgt cttccaggaa caaacgttga cttaggaact ttggaatctg
     3481 aagacctgat ccccctcttc aatgatgtag agtctgctct gaacaaaagt gagccctttc
     3541 taacctggct gtaagaattc cgcccccccc cctctccctc ccccccccct aacgttactg
     3601 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     3661 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     3721 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     3781 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     3841 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     3901 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     3961 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     4021 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     4081 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     4141 aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4201 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4261 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4321 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     4381 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4441 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4501 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4561 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     4621 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     4681 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4741 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     4801 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4861 gagctgtaca agggcagcgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag
     4921 gagaatcctg gacccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4981 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     5041 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     5101 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     5161 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     5221 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     5281 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     5341 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     5401 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     5461 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     5521 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     5581 ccccatgcat cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5641 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     5701 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5761 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5821 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5881 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     5941 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     6001 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6061 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     6121 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6181 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6241 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     6301 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     6361 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     6421 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     6481 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6541 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6601 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6661 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6721 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6781 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6841 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6901 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     6961 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7021 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7081 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7141 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7201 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7261 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7321 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7381 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7441 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7501 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7561 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7621 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7681 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7741 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7801 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7861 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7921 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7981 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8041 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8101 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8161 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8221 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8281 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8341 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8401 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8461 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8521 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8581 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8641 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8701 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8761 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8821 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8881 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8941 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9001 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9061 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     9121 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     9181 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     9241 tggtggaagt aaggtggtac gatcgtgcc
//
