LOCUS       sequence-340308-        2773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of NS1 Influenza A virus protein (strain 
            Texas91) for enhanced in vitro transcribed mRNA translation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2773)
  TITLE     Verstreken lab plasmids
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIVTR(T7G)-NS1-TX91
FEATURES             Location/Qualifiers
     source          1..2773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     rep_origin      complement(1018..1606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1098..1117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1777..2568,2569..2637))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2400..2419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2638..2742)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatacgact cactataggg acatttgctt ctgacacaac tgtgttcact agcaacctca
       61 aacagaattc gagctcggta cccggggatc ctctagagtc gccaccatgg atagcaatac
      121 agttagttct tttcaggtag attgttttct ctggcatgtt cgaaaacaag ttgcagacca
      181 agaacttggt gacgccccgt tccttgatag attgagaagg gaccaaaaat ccctgaaagg
      241 aaggggatca actttgggct tgaatatcga gaccgccacg tgtgtaggta agcagatagt
      301 cgaaagaata cttaaagaag agtccgacga ggcatttcga atgactatgg cctccgctct
      361 tgcctctcgc tatcttactg acatgacaat agaagaaatg agccgcgact ggtttatgct
      421 gatgcctaag cagaaagttg ccggaccttt gtgtgttcga atggaccagg ctatcatgga
      481 taagaacata attttgaaag ctaatttctc cgtaatcttc gaccgattgg agactctgac
      541 cttgcttcga gcgttcacgg aagagggggc catagtgggt gaaatttcac cactgcctag
      601 tctccccgga cacacgaacg aggatgtgaa aaacgcgatc ggtgtcctca tcggcggcct
      661 tgaatggaac gataatactg tccgggtaag cgagacgctc cagcgattcg catggcgatc
      721 atctaacgag aacggtggcc cccccttgac cccaactcaa aagagaaaga tggctgggaa
      781 gatacgctca gaagtttgag acctgcaggc atgcaagctt gctcgctttc ttgctgtcca
      841 atttctatta aaggttcctt tgttccctaa gtccaactac taaactgggg gatattatga
      901 agggccttga gcatctggat tctgcctaat aaaaaacatt tattttcatt gcaaaaccat
      961 gggagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1441 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1681 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1741 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1801 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1861 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1921 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1981 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2041 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2101 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2161 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2221 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2281 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2341 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2401 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2461 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2521 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2581 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     2641 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2701 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2761 gccacctgac gtc
//
