LOCUS       sequence-340316-       10940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed 
            from UAS enhancer. N-terminal secretion signal from Drosophila BiP. 
            C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Droujinine IA, Meyer AS, Wang D, Udeshi ND, Hu Y, Rocco D, McMahon 
            JA, Yang R, Guo J, Mu L, Carey DK, Svinkina T, Zeng R, Branon T, 
            Tabatabai A, Bosch JA, Asara JM, Ting AY, Carr SA, McMahon AP, 
            Perrimon N
  TITLE     Proteomics of protein trafficking by in vivo tissue-specific 
            labeling.
  JOURNAL   Nat Commun. 2021 Apr 22;12(1):2382. doi: 10.1038/s41467-021-22599-x.
  PUBMED    33888706
REFERENCE   2  (bases 1 to 10940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-04-22"; volume: "12(1):2382. doi"; pages: " 
            10.1038/s41467-021-22599-x"
COMMENT     Sequence Label: pUAS-Myc-BirA-G3-ER
FEATURES             Location/Qualifiers
     source          1..10940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..55
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    121..154
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    167..261
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        285..523
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     470..491
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    568..592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     sig_peptide     613..666
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             667..696
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             703..1659
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGKTGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(1694..1718)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          2025..2090
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2220..2240
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2512..2633
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2549..2568)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2603..2622
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2655..3085
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(3104..3124)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3104..3122)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3143..3159)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3143..3159)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3156..3178)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3167..3183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3191..3197,3198..3215,3216..3221))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3236..3257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3374..3391)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3545..4133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3625..3644)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4304..5095,5096..5164))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5165..5269)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5296..5752
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5383..5402)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5593..5614
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5878..5900
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5893..5909
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5919..5938
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5919..5937
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(6742..7356,7427..7558,7762..8077,8139..8793,
                     8868..9141,9543..9614))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     10066..10084
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    10207..10276
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    join(10514..10940,1..4)
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 caacactagt agtaccagct tataacttcg tataatgtat gctatacgaa gttatctgca
       61 ggcaggtcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag actcccatgg
      121 ataacttcgt ataatgtatg ctatacgaag ttatggatcc gcaggtcgga gtactgtcct
      181 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
      241 agcggagtac tgtcctccga gcggagactc gtcgacagcg agcgccggag tataaataga
      301 ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag
      361 cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc agtaaagtgc
      421 aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg caactactga
      481 aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg aattgggaat
      541 tccagatctg cggccgcggc tcgaatcaca agtttgtaca aaaaagcagg ctccgcggcc
      601 gcccccttca ccatgaagtt atgcatatta ctggccgtcg tggcctttgt tggcctctcg
      661 ctcggggaac aaaagcttat ttctgaagag gacttggcta gcaaagacaa tactgtgcct
      721 ctgaagctga tcgctctcct ggctaatggc gagttccata gtggcgaaca gctgggagaa
      781 accctgggca tgtccagggc cgctatcaac aagcacattc agactctgcg cgactggggc
      841 gtggacgtgt tcaccgtgcc cggaaagggc tactctctgc ccgagcctat cccgctgctg
      901 aacgctaaac agattctggg acagctggac ggcgggagcg tggcagtcct gcctgtggtc
      961 gactccacca atcagtacct gctggatcga atcggcgagc tgaagagtgg ggatgcttgc
     1021 attgcagaat atcagcaggc agggagagga agcagaggga ggaaatggtt ctctcctttt
     1081 ggagctaacc tgtacctgag tatgttttgg cgcctgaagc ggggaccagc agcaatcggc
     1141 ctgggcccgg tcatcggaat tgtcatggca gaagcgctgc gaaagctggg agcagacaag
     1201 gtgcgagtca aatggcccaa tgacctgtat ctgcaggata gaaagctggc aggcatcctg
     1261 gtggagctgg ccggaaaaac aggcgatgct gcacagatcg tcattggcgc cgggattaac
     1321 gtggctatga ggcgcgtgga ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa
     1381 gcagggatta acctggacag gaatactctg gccgctatgc tgatccgaga gctgcgggca
     1441 gccctggaac tgttcgagca ggaaggcctg gctccatatc tgtcacggtg ggagaagctg
     1501 gataacttca tcaatagacc cgtgaagctg atcattgggg acaaagagat tttcgggatt
     1561 agccggggga ttgataaaca gggagccctg ctgctggaac aggacggagt tatcaaaccc
     1621 tggatgggcg gagaaatcag tctgcggtct gccgaaaaga aggacgagct gtaatagaag
     1681 ggtgggcgcg ccgacccagc tttcttgtac aaagtggtga ttcgagggta cctctagagc
     1741 aaactagttc tgatctgcta gacaattgtt ggcatcaggt aggcatcaca cacgattaac
     1801 aacccctaaa aatacacttt gaaaatattg aaaatatgtt tttgtataca tttttgatat
     1861 tttcaaataa tacgcagtta taaaactcat tagctaaccc attttttctt tgcttatgct
     1921 tacagattgc aaagaactag agccgcggga tctttgtgaa ggaaccttac ttctgtggtg
     1981 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     2041 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     2101 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     2161 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     2221 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     2281 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     2341 aaaaagctgc actgctatac aagaaaatta tggaaaaata tttgatgtat agtgccttga
     2401 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     2461 ccacacctcc ccctgaacct gaaacataaa atgaatggaa ttgttgttgt taacttgttt
     2521 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2581 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     2641 tggttccaga gctctggcca cgtaataagt gtgcgttgaa tttattcgca aaaacattgc
     2701 atattttcgg caaagtaaaa ttttgttgca taccttatca aaaaataagt gctgcatact
     2761 ttttagagaa accaaataat tttttattgc atacccgttt ttaataaaat acattgcata
     2821 ccctctttta ataaaaaata ttgcatactt tgacgaaaca aattttcgtt gcatacccaa
     2881 taaaagatta ttatattgca tacccgtttt taataaaata cattgcatac cctcttttaa
     2941 taaagaatat tgcatacgtt gacgaaacaa attttcgttg catacccaat aaaagattat
     3001 tatattgcat accttttctt gccataccat ttagccgatc aattgtgctc ggcaacagta
     3061 tatttgtggt gtgccaacca acaacgagct ccagcttttg ttccctttag tgagggttaa
     3121 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     3181 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3241 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3301 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3361 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3421 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3481 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3541 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3601 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3661 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3721 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3781 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3841 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3901 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3961 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     4021 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4081 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4141 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4201 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4261 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4321 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4381 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4441 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4501 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4561 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4621 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4681 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4741 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4801 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4861 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4921 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4981 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5041 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5101 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5161 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5221 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5281 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5341 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5401 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5461 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5521 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5581 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5641 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aaatgagctg
     5701 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc
     5761 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     5821 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     5881 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     5941 aattgggtac aagcttcacg acctaggcgc gccctagttc cagtgaaatc caagcatttt
     6001 ctaaattaaa tgtattctta ttattatagt tgttattttt gatatatata aacaacacta
     6061 ttatgcccac catttttttg agatgcatct acacaaggaa caaacactgg atgtcacttt
     6121 cagttcaaat tgtaacgcta atcactccga acaggtcaca aaaaattacc ttaaaaagtc
     6181 ataatattaa attagaataa atatagctgt gagggaaata tatacaaata tattggagca
     6241 aataaattgt acatacaaat atttattact aatttctatt gagacgaaat gaaccactcg
     6301 gaaccatttg agcgaaccga atcgcgcgga actaacgaca gtcgctccaa ggtcgtcgaa
     6361 caaaaggtga atgtgttgcg gagagcgggt gggagacagc gaaagagcaa ctacgaaacg
     6421 tggtgtggtg gaggtgaatt atgaagaggg cgcgcgattt gaaaagtatg tatataaaaa
     6481 atatatcccg gtgttttatg tagcgataaa cgagtttttg atgtaaggta tgcaggtgtg
     6541 taagtctttt ggttagaaga caaatccaaa gtctacttgt ggggatgttc gaaggggaaa
     6601 tacttgtatt ctataggtca tatcttgttt ttattggcac aaatataatt acattagctt
     6661 tttgaggggg caataaacag taaacacgat ggtaataatg gtaaaaaaaa aaaacaagca
     6721 gttatttcgg atatatgtcg gctactcctt gcgtcgggcc cgaagtctta gagccagata
     6781 tgcgagcacc cggaagctca cgatgagaat ggccagaccc acgtagtcca gcggcagatc
     6841 ggcggcggag aagttaagcg tctccaggat gaccttgccc gaactggggc acgtggtgtt
     6901 cgacgatgtg cagctaattt cgcccggctc cacgtccgcc cattggttaa tcagcagacc
     6961 ctcgttggcg taacggaacc atgagaggta cgacaaccat ttgaggtata ctggcaccga
     7021 gcccgagttc aagaagaagc cgccaaagag caggaatggt atgataaccg gcggacccac
     7081 agacagcgcc atcgaggtcg aggagctggc gcaggatatt agatatccga aggacgttga
     7141 cacattggcc accagagtga ccagcgccag gcagttgaag aagtgcagca ctccggcccg
     7201 cagtccgatc atcggatagg caatcgccgt gaagaccagt ggcactgtga gaaaaagcgg
     7261 caattcggca atcgttttgc ccagaaagta tgtgtcacag cgataaagtc gacttcgggc
     7321 ctccctcata aaaactggca gctctgaggt gaacacctaa atcgaatcga ttcattagaa
     7381 agttagtaaa ttattgaaat gcaaatgtat tctaaacatg acttacattt atcgtggcaa
     7441 agacgttttg aaaggtcatg ttggtcagga agaggaagat ggctccgttg atattcatca
     7501 cacccacttg cgtgagttgt tggcccaaaa agatgaggcc aatcaagatg gcaaccatct
     7561 gcaaattaaa atgttactcg catctcatta atattcgcga gttaaatgaa atttatttat
     7621 cttctgcaaa actataaact atacatctca ttgaaaaaaa ctaagaaggg tgtggaatca
     7681 ggcaattcta tctaaaatct agcgaatttg tttccaagaa ttgtaagcgt tatatcattt
     7741 gtttccactg gaaccactca ccgttgtctg aataagtcgc acttttacga ggagtggttc
     7801 cttgagcacc gacagccagg atcgccacag gaccgcccgg aactgcatga accaggtggc
     7861 cttgtaggtg tacccattct ccggctgctc cagtggcttc tccagatttt tggtggccaa
     7921 caactgctcc atatcccggg ctactttgct aatggcaaaa ttgtcgcata tcttggcgat
     7981 ccgatcacgg gactcgatct cccgtccggg cacaacggcc aacacctgta cgtaaaagtc
     8041 cgccggattg tagttggtag gacactgggc acccacgctg gataggagtt gagatgttat
     8101 gtaatactag atacccttaa taaacacatc gaactcacta ggaaaagaag tcgacggctt
     8161 cgctgggagt gcccaagaaa gctaccctgc cctcggccat cagaaggatc ttgtcaaaga
     8221 gctcaaacag ctcggaagac ggctgatgaa tggtcaggat gacggtcttg cccttctgcg
     8281 acagcttctt cagcacctgg acgacgctgt gggcggtaaa ggagtccagt ccggaggtgg
     8341 gctcatcgca gatcagaagc ggcggatcgg ttagagcctc ggaggcgaat gccagacgct
     8401 tcctttctcc gccggacaga cctttcaccc tgccgggcac accgatgatc gtgtgctgac
     8461 atttgctgag cgaaagctcc tggatcacct gatccacgcg ggccactcgc tgccgatagg
     8521 tcagatgtcg tggcatccgc accatggctt ggaaaatcag gtgttccctg gccgttaggg
     8581 agccgataaa gaggtcatcc tgctggacat aggcgcacct ggcctgcatc tccttggcgt
     8641 ccacaggttg gccattgagc agtcgcatcc cggatggcga tacttggatg ccctgcggcg
     8701 atcgaaaggc aagggcattc agcagggtcg tctttccggc accggaactg cccatcacgg
     8761 ccaaaagttc gcccggatag gccacgccgc aaactgagtt tcaaattggt aattggaccc
     8821 tttattaaga tttcacacag atcagccgac tgcgaataga aactcaccgt tcttgagcaa
     8881 atgtttcctg ggcgccggta tgtgtcgctc gttgcagaat agtccgcgtg tccggttgac
     8941 cagctgccgc catccggagc ccggctgatt gaccgcccca aagatgtcca tattgtgcca
     9001 ggcataggtg aggttctcgg ctagttggcc gctccctgaa ccggagtcct ccggcggact
     9061 gggtggccgg agcgtgccgt agtttttggc ctgcccgaag ccctggttaa tgcagctctg
     9121 cgaagccgct ccgctgtcac cctgcaatga taggggatct caaatatcaa ctacaagcgt
     9181 tatgctcatc taaccccgaa caaaaagtac cccgaagtat cctacgaagt aggtttatac
     9241 ttttatttat tttttgtgca tctaggatca gcttaaaata tctggttgtt atattttttg
     9301 taaaaaagaa tatagtcgaa aatgaatgcc tttagatgtc ttgatcatga tatgatctca
     9361 aaaattgtct tatatagcga gaacagctac cagaataatc tgtttcgtgt cactatttgt
     9421 ttgtgcaatt gcggtttggg atttttgtgg gtcgcagttc tcacgccgca gacaatttga
     9481 tgttgcaatc gcagttccta tagatcaagt gaacttaaga tgtatgcaca tgtactactc
     9541 acattgttca gatgctcggc agatgggtgt ttgctgcctc cgcgaattaa tagctcctga
     9601 tcctcttggc ccattgccgg gatttttcac actttcccct gcttacccac ccaaaaccaa
     9661 tcaccacccc aatcactcaa aaaacaaaca aaaataagaa gcgagaggag ttttggcaca
     9721 gcactttgtg tttaattgat ggcgtaaacc gcttggagct tcgtcacgaa accgctgaca
     9781 aaatgcaact gaaggcggac attgacgcta cgtaacgcta caaacggtgg cgaaagagat
     9841 agcggacgca gcggcgaaag agacggcgat atttctgtgg acagagaagg aggcaaacag
     9901 cgctgacttt gagtggaatg tcattttgag tgagaggtaa tcgaaagaac ctggtacatc
     9961 aaataccctt ggatcgaagt aaatttaaaa ctgatcagat aagttcaatg atatccagtg
    10021 cagtaaaaaa aaaaaatgtt ttttttatct actttccgca aaaatgggtt ttattaactt
    10081 acatacatac taggcgcgcc agatcgcaag aagcttgata tcatcgatct cgaggctgca
    10141 tccaacgcgt tgggagctct ccggatcaat tcggcttcag gtaccgtcga cgatgtaggt
    10201 cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta
    10261 ctccacctca cccatctggt ccatcatgat gaacgggtcg aggtggcggt agttgatccc
    10321 ggcgaacgcg cggcgcaccg ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac
    10381 ggtgagcacg ggacgtgcga cggcgtcggc tggtgcggat acgcggggca gcgtcagcgg
    10441 gttctcgacg gtcacggcgg gcatgtcgac aagccgaatt gatccactag aaggcctaat
    10501 tcggtacact agttggccac gtaataagtg tgcgttgaat ttattcgcaa aaacattgca
    10561 tattttcggc aaagtaaaat tttgttgcat accttatcaa aaaataagtg ctgcatactt
    10621 tttagagaaa ccaaataatt ttttattgca tacccgtttt taataaaata cattgcatac
    10681 cctcttttaa taaaaaatat tgcatacttt gacgaaacaa attttcgttg catacccaat
    10741 aaaagattat tatattgcat acccgttttt aataaaatac attgcatacc ctcttttaat
    10801 aaagaatatt gcatacgttg acgaaacaaa ttttcgttgc atacccaata aaagattatt
    10861 atattgcata ccttttcttg ccataccatt tagccgatca attgtgctcg gcaacagtat
    10921 atttgtggtg tgccaaccaa
//
